Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 1, 2005

Dehydrogenation studies of dihydronicotinamide adenine dinucleotide (NADH) with methylene blue in the presence of the copper hexcyanoferrate(II) complex and light

Brij Tewari
From the journal Open Chemistry


The effects of copper ferrocyanide and light on the dehydrogenation rate of NADH by methylene blue is studied. The results suggest that the dehydrogenation rate of NADH with methylene blue is enhanced by copper ferrocyanide. Light also affects the reaction rate.

[1] S.L. Miller: “A production of amino acids under possible primitive earth condition”, Science, Vol. 117, (1953), pp. 528–529. in Google Scholar

[2] S.L. Miller: “Production of some organic compounds under primitive earth conditions”, J. Am. Chem. Soc., Vol. 77, (1955), pp. 2351–2361. in Google Scholar

[3] C. Sagan and B.N. Khare: “Long wavelength ultraviolet photoproduction of amino acids on the primitive earth”, Science, Vol. 173, (1971), pp. 417–420. in Google Scholar

[4] J. Takahashi, T. Hosokawa, H. Masuda, T. Kaneko, K. Kobayashi, T. Saito and Y. Utsumi: “Abiotic synthesis of amino acids by X-ray irradiation of simple inorganic gases”, Appl. Phys. Lett., Vol. 74, (1999), pp. 877–879. in Google Scholar

[5] J.F. Kasting, A.A. Pavlor and J.L. Siefert: “A coupled ecosystem-climate model for predicting the methane concentration in the archean atmosphere”, Origins Life Evol. Biosphere, Vol. 31, (2001), pp. 271–285. in Google Scholar

[6] J.F. Kasting: “Earth's early atmosphere”, Science, Vol. 259 (1993), pp. 920–926. in Google Scholar

[7] M.T. Beck: “Prebiotic coordination chemistry. The possible role of transition metal complexes in chemical evolution”, In: H. Sigel (Ed.): Metal Ions in Biological Systems, Vol. 7, Marcel Dekker, New York, 1978, p. 1. Search in Google Scholar

[8] Kamaluddin, M. Nath and A. Sharma: “Role of metal ferrocyanides in chemical evolution”, Origins Life Evol. Biosphere, Vol. 24, (1994), pp. 469–477. in Google Scholar

[9] B.B. Tewari and Kamaluddin: “Interaction of o-amniphenol and o-nitrophenol with copper, zinc, molybdenum and chromium ferrocyanides”, J. Colloid and Interface Sci., Vol. 193, (1997), pp. 167–171. in Google Scholar

[10] B.B. Tewari, D. Mohan and Kamaluddin: “Interaction of 2, 4-dinitrophenol and 2,4,6-trinitrophenol with copper, zinc, molybdenum and chromium ferrocyanides”, Colloid and Surfaces, Vol. 131, (1998), pp. 89–93. in Google Scholar

[11] L.H. Baetsle, D. Huys and D. Van Deyck: “Ferrocyanide molybdate, A new inorganic ion-exchanger”, J. Inorg. Nucl. Chem., Vol. 28, (1966), pp. 2385–2394. in Google Scholar

[12] W.U. Malik, S.K. Srivastava, B.M. Bhaudari and S. Kumar: “Ion-exchange properties of chromium ferrocyanide”, J. Inorg. Nucl. Chem., Vol. 38, (1976), pp. 342–343. in Google Scholar

[13] Y. Hino and S. Minakami: “Electron transport pathway of the NADH dependent haem oxygenase system of rat liver microsomal fraction induced by cobalt chloride”, Biochem. J., Vol. 178, (1979), pp. 323–329. Search in Google Scholar

[14] J. Moiroux and P.J. Elving: “Mechanistic aspects of the electrochemical oxidation of dihydro nicotinamide adenine dinucleotide (NADH)”, J. Am. Chem. Soc., Vol. 102, (1980), pp. 6533–6538. in Google Scholar

[15] Y.D. Wu and K.N. Houk: “Theoretical evaluation of conformational preferences of NAD+ and NADH: An approach to understanding the steriospecificity of NAD+/NADH-dependent dehydrogenases”, J. Am. Chem. Soc., Vol. 113, (1991), pp. 2353–2358. in Google Scholar

[16] K. Umeda, A. Nakamura and F. Toda: “Investigation on photochemical reduction of NAD+ to NADH in liposomal solution”, Chem. Lett., (1990), pp. 1433–1436. Search in Google Scholar

[17] T. Kajiki, N. Tamura, T. Nabeshima and Y. Yano: “Rate acceleration of the oxidation of an NADH model by flavin with a functionalized flavin receptor in chloroform”, Chem. Lett., (1995), pp. 1063–1064. Search in Google Scholar

[18] M. Murata, M. Kobayashi and S. Kawanishi: “Nonenzymatic reduction of nitroderivatives of a heterocyclic amine IQ by NADH and Cu(II) leads to oxidative DNA damage”, Biochemistry, Vol. 38 (1999), pp. 7624–7629. in Google Scholar PubMed

[19] M. Murray and A.M. Butler: “Hepatic biotransformation of parathion: Role of cytochrome p 450 in NADPH- and NADH—meditated microsomal oxidation in vitro”, Chem. Res. Toxicol. Vol. 7, (1994), pp. 792–799. in Google Scholar PubMed

[20] T. Iyanagi and K.F. Anan: “One electron oxidation—reduction properties of hepatic NADH—Cytochrome b5 reductase”, Biochemistry, Vol. 23, (1984) pp. 1418–1425. in Google Scholar PubMed

[21] H.A. Harper: Reviews of Physiological Chemistry, 14th ed., Lange Medical Publications, Los Altos, California, 1973, p. 100. Search in Google Scholar

[22] D.E. Metzler: Biochemistry, Academic Press, New York, 1977, p. 469. Search in Google Scholar

[23] A. Ciszewski and G. Milczarek: “Electrocatalysis of NADH oxidation with an electropolymerized film of 1,4-bis (3,4-dihydroxyphenyl)-2,3-dimethylbutane”, Anal. Chem., Vol. 72, (2000) pp. 3203–3209. in Google Scholar PubMed

[24] T.N. Rao, I. Yagi, T. Miwa, D.A. Tryk and A. Fujishima: “Electrochemical oxidation of NADH at highly boron-doped diamond electrodes”, Anal. Chem., Vol. 71, (1999), pp. 2506–2511. in Google Scholar PubMed

[25] G.D. Storrier, K. Takada and H.D. Abrana: “Catechol-pendant terpyridine complexes: electrodeposition studies and electrocatalysis of NADH oxidation”, Inorg. Chem., Vol. 38, (1999), pp. 559–565. in Google Scholar PubMed

[26] H.L. Levine and E.T. Kaiser: “Steriospecificity in the oxidation of NADH by flavopapaine”, J. Am. Chem. Soc., Vol. 102, (1980), pp. 343–345. in Google Scholar

[27] B.W. Carlson, L.L. Miller, P. Neta and J. Grodkowski: “Oxidation of NADH involving rate-limiting one electron transfer”, J. Am. Chem. Soc., Vol. 106, (1984), pp. 7233–7239. in Google Scholar

[28] C. Degrand and L.L. Miller: “An electrode modified with polymer-bound dopamine which catalyzes NADH oxidation”, J. Am. Chem. Soc., Vol. 102, (1980), pp. 5728–5732. in Google Scholar

[29] F. Ni, H. Feng, L. Gorton and T.M. Cotton: “Electrochemical and SERS studies of chemically modified electrodes: Nile Blue A, mediator for NADH oxidation”, Langmuir, Vol. 6, (1990), pp. 66–73. in Google Scholar

[30] A. Marcinek, J. Rogowski, J. Adamus, J. Gebicki, P. Bednarek and T. Bally “Hydroge-transferre radical cations of NDH model compound 2. Sequential electron-proton addition to NAD+”, J. Phys. Chem. A, Vol. 104, (2000), pp. 718–723. in Google Scholar

[31] A. Marcinek, J. Adamus, J. Gebicki, M.S. Platz and P. Bednarek: “Hydrogen transferred radical cation of NADH model compounds. 3. 1,8-acridinediones”, J. Phys. Chem. A, Vol. 104, (2000), pp. 724–728. in Google Scholar

[32] V. Kourim, J. Rais and B. Million: “Exchange properties of complex cyanides-I”, J. Inorg. Chem., Vol. 26, (1964), pp. 1111–1115. Search in Google Scholar

[33] W. Hücketl: Structural Chemistry of Inorganic Compounds, Vol. 1, Elsevier, Amsterdam, 1950. Search in Google Scholar

[34] K. Nakamoto, J. Fujita and H. Murata: “Infrared spectra of metallic complexes. V. The infrared spectra of nitro and nitrito complexes”, J. Am. Chem. Soc., Vol. 80, (1958), pp. 4817–4823. in Google Scholar

[35] P. Ratnasamy and A.J. Leonard: “Evolution of chromia”, J. Phys. Chem., Vol. 76, (1976), pp. 1938–1843. Search in Google Scholar

[36] B.B. Tewari and Kamaluddin: “Photo-sensitized oxidation of diphenylamine using nickel ferrocyanide and its relevance to chemical evolution”, In: Proceedings of Ninth National Space Science Symposium (NSSS-96), Osmania University, Hyderabad, India, 1996, p. 93. Search in Google Scholar

Published Online: 2005-9-1
Published in Print: 2005-9-1

© 2005 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Scroll Up Arrow