In February 1993, the group of Klaus Mosbach published their milestone study in Nature where, for the first time, non-covalent molecular imprints were employed in a competitive binding assay. In this seminal piece of work, and also for the first time, they refer to molecularly imprinted polymers as being ‘antibody mimics’ and hypothesised that these synthetic materials could one day provide ‘a useful, general alternative to antibodies’. This perspective article examines how far we have come in the 20 years since this publication in terms of realising this hypothesis and poses the question of whether we actually need molecularly imprinted polymers to be a general alternative to antibodies.
Wulff G. and Sarhan A., Use of polymers with enzymeanalogous structures for the resolution of racemates. Angew. Chem. Int. Ed. Engl., 1972, 11(4), 341. Search in Google Scholar
Vlatakis G., et al., Drug assay using antibody mimics made by molecular imprinting. Nature, 1993, 361(6413), 645-647. Search in Google Scholar
Alexander C., et al., Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003. J. Mol. Recognit., 2006, 19(2), 106-180. 10.1002/jmr.760Search in Google Scholar
MIP Database. (accessed: 05/02/2012); Available from: www.mipdatabase.com. Search in Google Scholar
Pichon V. and Chapuis-Hugon F., Role of molecularly imprinted polymers for selective determination of environmental pollutants - A review. Anal. Chim. Acta, 2008, 622(1-2), 48-61. 10.1016/j.aca.2008.05.057Search in Google Scholar
Manesiotis P., et al., Applications of SPE-MIP in the field of food analysis, in Comprehensive Sampling and Sample Preparation, J. Pawliszyn, Editor. 2012, Elsevier. 10.1016/B978-0-12-381373-2.00144-7Search in Google Scholar
Manesiotis P., Osmani Q., and McLoughlin P., An enantioselective chromatographic stationary phase for S-ibuprofen prepared by stoichiometric molecular imprinting. J. Mater. Chem., 2012, 22(22), 11201-11207. 10.1039/c2jm16659cSearch in Google Scholar
Sellergren B., ed. Molecularly Imprinted Polymers - Manmade mimics of antibodies and their applications in analytical chemistry. 2001, Elsevier. Search in Google Scholar
Coico R. and Sunshine G., Immunology: A Short Course. 6th ed. 2009: Wiley-Blackwell. Search in Google Scholar
Asanuma H., Hishiya T., and Komiyama M., Tailor-made receptors by molecular imprinting. Adv. Mater., 2000, 12(14), 1019-1030. 10.1002/1521-4095(200007)12:14<1019::AID-ADMA1019>3.0.CO;2-KSearch in Google Scholar
Manesiotis P., et al., An artificial riboflavin receptor prepared by a template analogue imprinting strategy. Angew. Chem., Int. Ed. Engl., 2005, 44(25), 3902-3906. 10.1002/anie.200500342Search in Google Scholar
Nicholls I.A. and Andersson H.S., Thermodynamic principles underlying molecularly imprinted polymer formulation and ligand recognition, in Molecularly Imprinted Polymers - Man-made Mimics of Antibodies and their Applications in Analytical Chemistry, B. Sellergren, Editor. 2001, Elsevier. 10.1016/S0167-9244(01)80006-8Search in Google Scholar
Bossi A., et al., Molecularly imprinted polymers for the recognition of proteins: The state of the art. Biosens. Bioelectron., 2007, 22(6), 1131-1137. 10.1016/j.bios.2006.06.023Search in Google Scholar
Hart B.R. and Shea K.J., Synthetic peptide receptors: Molecularly imprinted polymers for the recognition of peptides using peptide-metal interactions. J. Am. Chem. Soc., 2001, 123(9), 2072-2073. 10.1021/ja005661aSearch in Google Scholar
Titirici M.M., Hall A.J., and Sellergren B., Hierarchical imprinting using crude solid phase peptide synthesis products as templates. Chem. Mater., 2003, 15(4), 822-824. 10.1021/cm025770jSearch in Google Scholar
Rachkov A. and Minoura N., Recognition of oxytocin and oxytocin-related peptides in aqueous media using a molecularly imprinted polymer synthesized by the epitope approach. J. Chromatogr. A., 2000, 889(1-2), 111-118. 10.1016/S0021-9673(00)00568-9Search in Google Scholar
Rachkov A. and Minoura N., Towards molecularly imprinted polymers selective to peptides and proteins. The epitope approach. Biochim. Biophys. Acta, 2001, 1544(1-2), 255- 266. 10.1016/S0167-4838(00)00226-0Search in Google Scholar
Hoshino Y., et al., Recognition, neutralization, and clearance of target peptides in the bloodstream of living mice by molecularly imprinted polymer nanoparticles: A plastic antibody. J. Am. Chem. Soc., 2010, 132(19), 6644- 6645. 10.1021/ja102148fSearch in Google Scholar PubMed PubMed Central
Hoshino Y., et al., Design of synthetic polymer nanoparticles that capture and neutralize a toxic peptide. Small, 2009, 5(13), 1562-1568. 10.1002/smll.200900186Search in Google Scholar PubMed PubMed Central
©2013 Versita Sp. z o.o.
This content is open access.