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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access March 1, 2007

Determination of active components in Chinese medicinal preparations by capillary electrophoresis

  • Huitao Liu EMAIL logo and Yuan Gao
From the journal Open Chemistry


The determination of paeoniforin, paeonol, and censenoside Rg1 in traditional Chinese medicinal preparations, Tze Po San Pien pills and Liuwen Dihuang pills has been investigated by micellar electrokinetic capillary electrophoresis with borate buffer (20 mM), sodium dodecylsulfate (30 mM) and acetonitrile (20%) as background electrolytes (pH 9.30), 20 kV applied voltage and 203 nm UV detection. The effects of SDS concentration, borate, buffer pH, and organic modifier on electrophoretic behavior and separation are discussed. Regression equations revealed linear relationships between the peak-area of each component and the content with the correlation coefficients from 0.9982 to 0.9999. In addition, the levels of the active compounds in two kinds of traditional Chinese medicinal preparations were easily determined with the recoveries from 93.1% to 108.2%.

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Published Online: 2007-3-1
Published in Print: 2007-3-1

© 2005 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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