Abstract
The contact angles of protein Newton black foam films from ALG (alpha-lactalbumin), BLG (beta-lactoglobulin) and BSA (bovine serum albumin) are measured here within. The measurements are carried out under dynamic and equilibrium conditions. For all proteins, a strong hystheresis effect of the contact angle is observed under dynamic conditions. An attempt is made to explain these results by the slow adsorption and desorption kinetics of the protein bilayers and by the dynamic structure and the rheology of the protein network forming the bubble walls. In addition, we propose a modification of the experimental device reported previously for contact angle measurements of large flat films in equilibrium. The advantages of this method are discussed in detail. Some shortcomings (precision, reproducibility) of this preliminary variant of the device in this initial stage of its application, do not allow one to draw reliable conclusions about the interactions of these films. Some improvements of the measurement quality are proposed. 
[1] G. Cevc and D. Marsh: Phospholipid Bilayers: Physical Principals and Models, John Wiley and Sons, New York, 1987. Search in Google Scholar
[2] D. Exerowa and P.M. Kruglyakov: Foam and foam films: Theory, experiment application, Elsevier, Amsterdam, 1998. Search in Google Scholar
[3] D. Exerowa: “Chain-melting phase transition and short-range molecular interactions in phospholipid foam bilayers”, Adv. Colloid Interf. Sci., Vol. 96, (2002), pp. 75–100. http://dx.doi.org/10.1016/S0001-8686(01)00076-810.1016/S0001-8686(01)00076-8Search in Google Scholar
[4] R. Cohen, D. Exerowa, T. Kolarov, et al.: “Thickness transitions in lysolecithin foam films”, Colloid Surface A., Vol. 65, (1992), pp. 201–209. http://dx.doi.org/10.1016/0166-6622(92)80275-710.1016/0166-6622(92)80275-7Search in Google Scholar
[5] A. Nikolova, R. Koinova, B. Tenchov, D. Exerowa: “Chain-melting phase transition in dipalmitoylphosphatidylcholine foam bilayers”, Chem. Phys. Lipids, Vol. 83, (1996), pp. 111–121. http://dx.doi.org/10.1016/0009-3084(96)02600-X10.1016/0009-3084(96)02600-XSearch in Google Scholar
[6] T. Yamanaka, M. Nayashi and R. Matuura: “Studies on the soap films stabilized by phospholipids: I. Effects of metal ions on the free energy of black soap films”, J. Colloid Interf. Sci., Vol. 88, (1982), pp. 458–466. http://dx.doi.org/10.1016/0021-9797(82)90274-010.1016/0021-9797(82)90274-0Search in Google Scholar
[7] J.L. Toca-Herrera, H.J. Müller, R. Krustev, D. Exerowa and H. Möhwald: “Influence of Na+, Ca2+ on the thickness and free energy of dmpc foam films”, Colloids Surface A, Vol. 144, (1998), pp. 319–326. http://dx.doi.org/10.1016/S0927-7757(98)00544-510.1016/S0927-7757(98)00544-5Search in Google Scholar
[8] J.L. Toca-Herrera, H.J. Müller, R. Krustev, T. Pfohl and H. Möhwald: “Influence of ethanol on the thickness and free energy of film formation of DMPC foam films”, Colloids Surface A, Vol. 152, (1999), pp. 357–365. http://dx.doi.org/10.1016/S0927-7757(98)00871-110.1016/S0927-7757(98)00871-1Search in Google Scholar
[9] J. Brezesinski, H.J. Müller, J.L. Toca-Herrera and R. Krustev: “X-ray diffraction and foam film investigations of PC head group interaction in water/ethanol mixtures”, Chem. Phys. Lipids, Vol. 110, (2001), pp. 183–194. http://dx.doi.org/10.1016/S0009-3084(01)00135-910.1016/S0009-3084(01)00135-9Search in Google Scholar
[10] J.-J. Benattar, M. Nedyalkov, J. Prost, A. Tiss, R. Verger and C. Guilbert: “Insertion Process of a Protein Single Layer within a Newton Black Film”, Phys. Rev. Lett., Vol. 82, (1999), pp. 5297–5300. http://dx.doi.org/10.1103/PhysRevLett.82.529710.1103/PhysRevLett.82.5297Search in Google Scholar
[11] V. Petkova, C. Sultanem, M. Nedyalkov, J.-J. Benattar, M. Leser and Ch. Schmitt: “Structure of a Freestanding Film of Beta-Lactoglobulin”, Langmuir, Vol. 19, (2003), pp. 6942–6949. http://dx.doi.org/10.1021/la027025r10.1021/la027025rSearch in Google Scholar
[12] A. Scheludko: “Thin liquid films”, Adv. Colloid Interf. Sci., Vol. 1, (1967), pp. 391–464. http://dx.doi.org/10.1016/0001-8686(67)85001-210.1016/0001-8686(67)85001-2Search in Google Scholar
[13] I.S. Clunie, Y.F. Goodman and B.T. Yngram: “Thin Liquid Films”, In: E. Matijevich (Ed.): Surface and Colloid Science, Vol. 3, Wiley, New York, 1971, p. 167. Search in Google Scholar
[14] D. Exerowa and A. Nikolova: “Short-range Molecular Interactions and Stability of Amphiphilic Bilayers”, J. Dispers. Sci. Technol., Vol. 18, (1997), pp. 683–690. http://dx.doi.org/10.1080/0193269970894376610.1080/01932699708943766Search in Google Scholar
[15] A. Nikolova and D. Exerowa: “Phase transitions in phosphatidylcholine foam bilayers”, J. Stat. Phys., Vol. 78, (1995), pp. 147–160. http://dx.doi.org/10.1007/BF0218334310.1007/BF02183343Search in Google Scholar
[16] D. Exerowa, A. Nikolova: “Phase transitions in phospholipid foam bilayers”, Langmuir, Vol. 8, (1992), pp. 3102–3108. http://dx.doi.org/10.1021/la00048a04110.1021/la00048a041Search in Google Scholar
[17] R. Cohen, R. Koynova, B. Tenchov and D. Exerowa: “Direct measurement of interaction forces in free thin liquid films stabilized with phosphatidylcholine”, Eur. Biophys. J., Vol. 20, (1991), pp. 203–208. http://dx.doi.org/10.1007/BF0018345610.1007/BF00183456Search in Google Scholar
[18] N. Cuvillier, F. Milett, V. Petkova, M. Nedyalkov and J.-J. Benattar: “Structure of Freestanding Phospholipidic Bilayer Films”, Langmuir, Vol. 16, (2000), pp. 5029–5035. http://dx.doi.org/10.1021/la990745m10.1021/la990745mSearch in Google Scholar
[19] N. Cuvillier, V. Petkova, M. Nedyalkov, F. Milett and J.-J. Benattar: “Protein insertion within a biological freestanding film”, Physica B, Vol. 283, (2000), p. 1–5. http://dx.doi.org/10.1016/S0921-4526(99)01880-310.1016/S0921-4526(99)01880-3Search in Google Scholar
[20] V. Petkova, M. Nedyalkov and J.-J. Benattar: “Freestanding black films of phos pholipids and phospholipid with proteins”, Colloid Surface A, Vol. 190, (2001), pp. 9–16. http://dx.doi.org/10.1016/S0927-7757(01)00660-410.1016/S0927-7757(01)00660-4Search in Google Scholar
[21] V. Petkova, J.-J. Benattar and M. Nedyalkov: “How to Control the Molecular Architecture of a Monolayer of Proteins Supported by a Lipid Bilayer”, Biophys. J., Vol. 82, (2002), pp. 541–548. Search in Google Scholar
[22] D. Platikanov, M. Nedyalkov and V. Petkova: “Phospholipid black foam films: dynamic contact angles and gas permeability of DMPC bilayer films”, Adv. Colloid Interf. Sci., Vol. 100-102, (2003), pp. 185–203. http://dx.doi.org/10.1016/S0001-8686(02)00057-X10.1016/S0001-8686(02)00057-XSearch in Google Scholar
[23] M. Nedyalkov, V. Petkova and D. Platikanov: “Phospholipid black foam films: dynamic film tension of DMPC bilayer films”, Colloid Surface A, Vol. 220, (2003), pp. 35–43. http://dx.doi.org/10.1016/S0927-7757(03)00058-X10.1016/S0927-7757(03)00058-XSearch in Google Scholar
[24] K. Mysels, H. Huisman and R. Razouk: “Measurement of Contact Angle between Thin Film and Bulk of Same Liquid”, J. Phys. Chem., Vol. 70, (1966), pp. 1339–1340. http://dx.doi.org/10.1021/j100876a51910.1021/j100876a519Search in Google Scholar
[25] D. Platikanov, G.P. Yampolskaya, N.I. Rangelova, Z.K Angarska, L.E. Bobrova and V.N. Izmailova: “Free Black Protein Films 2. Thermodynamic Parameters”, Colloid. J. USSR, Vol. 43, (1981), pp. 149–153. Search in Google Scholar
[26] D. Platikanov, M. Nedyalkov and V. Nasteva: “Line tension of Newton black films. I. Determination by the critical bubble method”, J. Colloid Interf. Sci., Vol. 75(2), (1980), pp. 612–619. http://dx.doi.org/10.1016/0021-9797(80)90484-110.1016/0021-9797(80)90484-1Search in Google Scholar
[27] V. Petkova, D. Platikanov and M. Nedyalkov: “Phospholipid black foam films: dynamic contact angles and gas permeability of DMPC+DMPG black films”, Adv. Colloid Interf. Sci., Vol. 104, (2003), pp. 37–51. http://dx.doi.org/10.1016/S0001-8686(03)00035-610.1016/S0001-8686(03)00035-6Search in Google Scholar
[28] T. Kolarov, Z. Zorin and D. Platikanov: “Profile of the transition region between aqueous wetting films on quartz and the adjacent meniscus”, Colloid Surface, Vol. 51, (1990), pp. 37–47. http://dx.doi.org/10.1016/0166-6622(90)80130-V10.1016/0166-6622(90)80130-VSearch in Google Scholar
[29] H.M. Princen and S.G. Mason: “Shape of a fluid drop at a fluid-liquid interface. I. Extension and test of two-phase theory”, J. Colloid Sci., Vol. 20, (1965), pp. 156–172. http://dx.doi.org/10.1016/0095-8522(65)90005-X10.1016/0095-8522(65)90005-XSearch in Google Scholar
[30] B.V. Derjaguin, G.A. Martynov, Yu.V. Gutop: “Thermodynamics and stability of free films”, Kolloidny Zhur., Vol. 27, (1965), p.357–365. Search in Google Scholar
[31] D. Platikanov, M. Nedyalkov and V. Nasteva: “Line tension of Newton black films. II. Determination by the diminishing bubble method”, J. Colloid Interf. Sci., Vol. 75(2), (1980), pp. 620–628. http://dx.doi.org/10.1016/0021-9797(80)90485-310.1016/0021-9797(80)90485-3Search in Google Scholar
[32] M. Nedyalkov and D. Platikanov: “Dependence of the Contact Angle on the Radius of Newton Black Films and the Line Tension”, Abhandlungen Akad Wissensch DDR, Vol. 1, (1985), pp. 123. Search in Google Scholar
[33] E. Evans: “Analysis of adhesion of large vesicles to surfaces”, Biophys. J., Vol. 31, (1980), p. 425–431. Search in Google Scholar
[34] E. Evans: “Structure and dynamics of membranes: Generic and specific interaction”, In: Lipowsky Sackmann (Ed.): Handbook of Biological Physics, Vol. 1B, Nord Holand, 1995, p. 723. Search in Google Scholar
[35] F. Pincet, E. Perez, J. Loudet and L. Lebeau: “From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory”, Phys. Rev. Lett., Vol. 87(17), (2001), p. 178101–178105. http://dx.doi.org/10.1103/PhysRevLett.87.17810110.1103/PhysRevLett.87.178101Search in Google Scholar
[36] D. Tareste, F. Pincet, E. Perez, S. Riskling, C. Mioskowski and L. Lebeau: “Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers”, Biophys. J., Vol. 83, (2002), p. 3675–3681. http://dx.doi.org/10.1016/S0006-3495(02)75367-810.1016/S0006-3495(02)75367-8Search in Google Scholar
[37] B. Derjaguin and A. Titievskaya: “Disjoing action of free liquid films and its contribution to the stability of foams”, Colloid J. USSR, Vol. 15, (1953), pp. 431–439. Search in Google Scholar
[38] D. Platikanov, M. Nedyalkov and N. Rangelova: “Measurement of the film tension of foam films in dynamic conditions”, Colloid Polym. Sci., Vol. 269, (1991), pp. 272–277. http://dx.doi.org/10.1007/BF0066550110.1007/BF00665501Search in Google Scholar
[39] D. Platikanov, M. Nedyalkov and N. Rangelova: “Method for direct measurement of the film tension of black foam films at various capillary pressures”, Colloid Polym. Sci., Vol. 265, (1987), pp. 72–75. http://dx.doi.org/10.1007/BF0142266710.1007/BF01422667Search in Google Scholar
[40] C. Sultanem: Films et bulles de proteins solubles: Structure, interactions et permeabilite au gaz, Thesis (PhD), Paris XI, Orsay, France, 2004. Search in Google Scholar
[41] J. van Steveninck, J.P.J. Boegheim and T.M.A.R. Dubbelman: “The influence of cupric ions on porphyrin-induced photodynamic membrane damage in human red blood cells”, Biochim. Biophys. Acta, Vol. 821, (1985), p. 1–7. http://dx.doi.org/10.1016/0005-2736(85)90146-410.1016/0005-2736(85)90146-4Search in Google Scholar
[42] M.A. Bos and T. Nylander: “Interaction between Beta-Lactoglobulin and Phospholipids at the Air/Water Interface”, Langmuir, Vol. 12, (1996), pp. 2791–2797. http://dx.doi.org/10.1021/la950640t10.1021/la950640tSearch in Google Scholar
[43] M. Cornec, D. Cho and G. Narsimhan: “Adsorption Dynamics of α-Lactalbumin and α-Lactoglobulin at Air-Water Interfaces”, J. Colloid Interf. Sci., Vol. 214, (1999), pp. 129–142. http://dx.doi.org/10.1006/jcis.1999.623010.1006/jcis.1999.6230Search in Google Scholar
[44] N.M. Princen: “Contact angles and transition regions in soap films”, J. Phys. Chem., Vol. 72, (1968), pp. 3342–3345. http://dx.doi.org/10.1021/j100855a05010.1021/j100855a050Search in Google Scholar
[45] O. Belorgey and J.-J. Benattar: “Structural properties of soap black films investigated by x-ray reflectivity”, Phys. Rev. Lett., Vol. 66, (1991), pp. 313–316. http://dx.doi.org/10.1103/PhysRevLett.66.31310.1103/PhysRevLett.66.313Search in Google Scholar
[46] N.I. Rangelova, V.N. Izmailova, D.N. Platikanov, G.P. Yampolskaya and Z.D. Tulovskaya: “Free Black Films of Proteins: 3. Dynamic Hysteresis of the Contact Angle (Film-Bulk Liquid) and the Rheological Properties of Adsorption Layers”, Colloid J. USSR, Vol. 52, (1990), pp. 442–445. Search in Google Scholar
[47] L.G. Cascao et al.: “A bike-wheel microcell for measurement of thin-film forces”, Colloids Surfaces A, Vol. 186, (2001), pp. 103–111. http://dx.doi.org/10.1016/S0927-7757(01)00488-510.1016/S0927-7757(01)00488-5Search in Google Scholar
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