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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 12, 2007

Interaction of unmodified and partially silylated nanosilica with red blood cells

Vladimir Gun’ko, Natalia Galagan, Irina Grytsenko, Vladimir Zarko, Olena Oranska, Vyacheslav Osaulenko, Viktor Bogatyrev and Vladimir Turov
From the journal Open Chemistry


Interaction of red blood cells (RBCs) with unmodified and partially (50%) silylated fumed silica A-300 (nanosilica)was studied by microscopic, XRD and thermally stimulated depolarisation current (TSDC) methods. Nanosilica at a low concentration C A-300C A-300 = 1 wt% all RBCs transform into shadow corpuscles because of 100% haemolysis. Partial (one-half) hydrophobization of nanosilica leads to reduction of the haemolytic effect in comparison with unmodified silica at the same concentrations. A certain portion of the TSDC spectra of the buffered suspensions with RBC/A-300 is independent of the amounts of silica. However, significant portions of the low-and high-temperature TSDC bands have a lower intensity at C A-300 = 1 wt% than that for RBCs alone or RBC/A-300 at C A-300 = 0.01 wt.% because of structural changes in RBCs. Results of microscopic and XRD investigations and calculations using the TSDC-and NMR-cryoporometry suggest that the intracellular structures in RBCs (both organic and aqueous components) depend on nanosilica concentration in the suspension.

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Published Online: 2007-9-12
Published in Print: 2007-12-1

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