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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 12, 2007

Interaction of unmodified and partially silylated nanosilica with red blood cells

Vladimir Gun’ko, Natalia Galagan, Irina Grytsenko, Vladimir Zarko, Olena Oranska, Vyacheslav Osaulenko, Viktor Bogatyrev and Vladimir Turov
From the journal Open Chemistry

Abstract

Interaction of red blood cells (RBCs) with unmodified and partially (50%) silylated fumed silica A-300 (nanosilica)was studied by microscopic, XRD and thermally stimulated depolarisation current (TSDC) methods. Nanosilica at a low concentration C A-300C A-300 = 1 wt% all RBCs transform into shadow corpuscles because of 100% haemolysis. Partial (one-half) hydrophobization of nanosilica leads to reduction of the haemolytic effect in comparison with unmodified silica at the same concentrations. A certain portion of the TSDC spectra of the buffered suspensions with RBC/A-300 is independent of the amounts of silica. However, significant portions of the low-and high-temperature TSDC bands have a lower intensity at C A-300 = 1 wt% than that for RBCs alone or RBC/A-300 at C A-300 = 0.01 wt.% because of structural changes in RBCs. Results of microscopic and XRD investigations and calculations using the TSDC-and NMR-cryoporometry suggest that the intracellular structures in RBCs (both organic and aqueous components) depend on nanosilica concentration in the suspension.

[1] M.T. Alyushin and M.N. Astakhova: “Aerosil and its application in pharmaceutical practice”, Pharmacy, Vol. 6, (1971), pp. 73–77. Search in Google Scholar

[2] A.A. Chuiko (Ed.): Medical chemistry and clinical application of silicon dioxide, Nukova Dumka, Kiev, 2003. Search in Google Scholar

[3] M.M. Kozlov and V.S. Markin: “Membranaceous skeleton of erythrocyte. Theoretical model”, Biolog. Membran., Vol. 3(4), (1986), pp. 404–421 (in Russian). Search in Google Scholar

[4] L.D. Krymsky, G.V. Nestaiko and A.G. Rybakov: Raster Electronic Microscopy of Vessels and Blood, Medicine, Moscow, 1976. Search in Google Scholar

[5] V.A. Muromtsev and V.N. Kidalov: Medicine in 21 Century, INTAN, St. Petersburg, 1998. Search in Google Scholar

[6] S.V. Rudenko, J.H. Crowe and F. Tablin: “Change in the erythrocyte shape depending on the term”, Biochemistry, Vol. 63, (1998), pp. 1630–1639. Search in Google Scholar

[7] J.D. Harley and J. Margolis: “Hemolytic activity of colloidal silica”, Nature, Vol. 189, (1961), pp. 1010–1011. http://dx.doi.org/10.1038/1891010a010.1038/1891010a0Search in Google Scholar

[8] I. Lindquist, O. Nillson and G. Ronquist: “Ultrastructural changes of erythrocyte membranes isolated with colloidal silica solution (ludox)”, Ups. J. Med. Sci., Vol. 79, (1974), pp. 1–6. http://dx.doi.org/10.3109/0300973740917838310.3109/03009737409178383Search in Google Scholar

[9] L.V. Lysenko, V.I. Chueshov and T.T. Lavrushina: “Comparative toxicity of modified samples of Aerosil”, Pharmac., Vol. 26, (1977), pp. 56–58 (in Russian). Search in Google Scholar

[10] B.I. Gerashchenko, V.M. Gun’ko, I.I. Gerashchenko, R. Leboda, H. Hosoya and I.F. Mironyuk: “Probing the silica surfaces by red blood cells”, Cytometry, Vol. 49(2), (2002), pp. 56–61. http://dx.doi.org/10.1002/cyto.1014610.1002/cyto.10146Search in Google Scholar

[11] J.P. Blitz and V.M. Gun’ko (Eds.): Surface Chemistry in Biomedical and Environmental Science, NATO Science Series II: Mathematics, Physics and Chemistry, Vol. 228, Springer, Dordrecht, 2006. Search in Google Scholar

[12] M. Diociaiuti, F. Bordi, L. Gataleta, G. Baldo, P. Crateri and L. Paoletti: “Morphological and functional alterations of human erythrocytes induced by SiO2 particles: an electron microscopy and dielectric spectroscopy study”, Environ. Res. A, Vol. 80, (1999), pp. 197–207. http://dx.doi.org/10.1006/enrs.1998.389210.1006/enrs.1998.3892Search in Google Scholar

[13] B.I. Gerashchenko, I.I. Gerashchenko and C.G. Pantazis: “Possible selective elimination of red blood cells under the influence of colloidal silica”, Med. Hypotheses, Vol. 47, (1996), pp. 69–70. http://dx.doi.org/10.1016/S0306-9877(96)90046-810.1016/S0306-9877(96)90046-8Search in Google Scholar

[14] B.I. Gerashchenko, I.I. Gerashchenko, V.I. Bogomaz and C.G. Pantazis: “Adsorption of Aerosil on erythrocyte surface by flow cytometry measurement”, Cytometry, Vol. 15, (1994), pp. 80–83. http://dx.doi.org/10.1002/cyto.99015011310.1002/cyto.990150113Search in Google Scholar PubMed

[15] V.M. Gun’ko, V.V. Turov, V.M. Bogatyrev, B. Charmas, J. Skubiszewska-Zięba, R. Leboda, S.V. Pakhovchishin, V.I. Zarko, L.V. Petrus, O.V. Stebelska and M.D. Tsapko: “Influence of partial hydrophobization of fumed silica by hexamethyldisilazane on interaction with water”, Langmuir, Vol. 19, (2003), pp. 10816–10828. http://dx.doi.org/10.1021/la030123810.1021/la0301238Search in Google Scholar

[16] V.V. Turov, V.M. Gun’ko, M.D. Tsapko, V.M. Bogatyrev, J. Skubiszewska-Zięba, R. Leboda and J. Riczkowski: “Influence of organic solvents on interfacial water at surfaces of silica gel and partially silylated fumed silica”, Appl. Surf. Sci., Vol. 229, (2004), pp. 197–213. http://dx.doi.org/10.1016/j.apsusc.2004.01.07010.1016/j.apsusc.2004.01.070Search in Google Scholar

[17] V.V. Turov, V.M. Gun’ko, V.M. Bogatyrev, V.I. Zarko, S.P. Gorbik, E.M. Pakhlov, R. Leboda, O.V. Shulga and A.A. Chuiko: “Structured water in partially dehydrated yeast cells and at partially hydrophobized fumed silica surface”, J. Colloid Interf. Sci., Vol. 283, (2005), pp. 329–343. http://dx.doi.org/10.1016/j.jcis.2004.09.04610.1016/j.jcis.2004.09.046Search in Google Scholar PubMed

[18] V.M. Gun’ko, V.V. Turov, V.M. Bogatyrev, V.I. Zarko, R. Leboda, E.V. Goncharuk, A.A. Novza, A.V. Turov and A.A. Chuiko: “Unusual properties of water at hydrophilic/hydrophobic interfaces”, Adv. Colloid Interf. Sci., Vol. 118, (2005), pp. 125–172. Search in Google Scholar

[19] V.V. Turov, V.M. Gun’ko, V.I. Zarko, R. Leboda, M. Jabłoński, M. Gorzelak and E. Jagiełło-Wojtowicz: “Weakly and strongly associated nonfreezable water bound in bones”, Colloids Surface. B, Vol. 48, (2006), pp. 167–175. http://dx.doi.org/10.1016/j.colsurfb.2006.02.00110.1016/j.colsurfb.2006.02.001Search in Google Scholar PubMed

[20] L.I. Mikhalovska, V.M. Gun’ko, V.V. Turov, V.I. Zarko, S.L. James, P. Vadgama, P.E. Tomlins and S.V. Mikhalovsky: “Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water”, Biomaterials, Vol. 27, (2006), pp. 3599–3607. Search in Google Scholar

[21] G.M. Sesler (Ed.): Electrets, Topics in Applied Physics, Vol. 33, Springer, Berlin, 1980. 10.1007/978-3-540-34846-7Search in Google Scholar

[22] M. Reichle, T. Nedetzka, A. Mayer and H. Vogel: “Dielectric properties of hydrated lyophilized hemoglobin as determined with the method of thermally stimulated depolarization”, J. Phys. Chem., Vol. 74, (1970), pp. 2659–2666. http://dx.doi.org/10.1021/j100707a01210.1021/j100707a012Search in Google Scholar

[23] P. Pissis, A.A. Konsta, S. Ratkovic, S. Todorovic and J. Laudat: “Temperatureand hydration-dependence of molecular mobility in seeds”, J. Therm. Anal., Vol. 47, (1996), pp. 1463–1483. http://dx.doi.org/10.1007/BF0199284010.1007/BF01992840Search in Google Scholar

[24] V.M. Gun’ko, V.I. Zarko, E.V. Goncharuk, L.S. Andriyko, V.V. Turov, Y.M. Nychiporuk, R. Leboda, J. Skubiszewska-Zięba, A.L. Gabchak, V.D. Osovskii, Y.G. Ptushinskii, G.R. Yurchenko, O.A. Mishchuk, P.P. Gorbik, P. Pissis and J.P. Blitz: “TSDC spectroscopy of relaxational and interfacial phenomena”, Adv. Colloid Interf. Sci., Vol. 131, (2007), pp. 1–89. http://dx.doi.org/10.1016/j.cis.2006.11.00110.1016/j.cis.2006.11.001Search in Google Scholar

[25] S.W. Provencher: “A constrained regularization method for inverting data represented by linear algebraic or integral equations”, Comput. Phys. Commun., Vol. 27, (1982), pp. 213–227. http://dx.doi.org/10.1016/0010-4655(82)90173-410.1016/0010-4655(82)90173-4Search in Google Scholar

[26] J. Gimsa, Th. Schnelle, G. Zechel and R. Glaser: “Dielectric spectroscopy of human erythrocytes: investigations under the influence of nystatin”, Biophys. J., Vol. 66, (1994), pp. 1244–1253. Search in Google Scholar

[27] J. Gimsa, T. Müller, T. Schnelle and G. Fuhr: “Dielectric spectroscopy of single human erythrocytes at physiological ionic strength: dispersion of the cytoplasm”, Biophys. J., Vol. 71, (1996), pp. 495–506. http://dx.doi.org/10.1016/S0006-3495(96)79251-210.1016/S0006-3495(96)79251-2Search in Google Scholar

[28] V.L. Sukhorukov and U. Zimmermann: “Electrorotation of erythrocytes treated with dipicrylamine: mobile charges within the membrane show their ’signature’ in rotational spectra”, J. Membrane Biol., Vol. 153, (1996), pp. 161–169. http://dx.doi.org/10.1007/s00232990011910.1007/s002329900119Search in Google Scholar

[29] R.D. Fomekong, U. Pliquett and F. Pliquett: “Passive electrical properties of RBC suspensions: changes due to distribution of relaxation times in dependence on the cell volume fraction and medium conductivity”, Bioelectroch. Bioener., Vol. 47 (1998) pp. 81–88. http://dx.doi.org/10.1016/S0302-4598(98)00161-510.1016/S0302-4598(98)00161-5Search in Google Scholar

[30] J.H. Strange, M. Rahman and E.G. Smith: “Characterisation of porous solids by NMR”, Phys. Rev. Lett., Vol. 71, (1993), pp. 3589–3591. http://dx.doi.org/10.1103/PhysRevLett.71.358910.1103/PhysRevLett.71.3589Search in Google Scholar

[31] J.H. Strange, J. Mitchell and J.B.W. Webber: “Pore surface exploration by NMR”, Magn. Reson. Imaging, Vol. 21, (2003), pp. 221–226. http://dx.doi.org/10.1016/S0730-725X(03)00128-010.1016/S0730-725X(03)00128-0Search in Google Scholar

[32] D.P. Gallegos, K. Munn, D.M. Smith and D.L. Stermer: “A NMR technique for the analysis of pore structure: application to materials with well defined pore structure”, J. Colloid Interf. Sci., Vol. 119, (1986), pp. 127–140. http://dx.doi.org/10.1016/0021-9797(87)90251-710.1016/0021-9797(87)90251-7Search in Google Scholar

[33] D.W. Aksnes and L. Kimtys: “1H and 2H NMR studies of benzene confined in porous solids: melting point depression and pore size distribution”, Solid State Nuc. Mag., Vol. 25, (2004), pp. 146–152. http://dx.doi.org/10.1016/j.ssnmr.2003.03.00110.1016/j.ssnmr.2003.03.001Search in Google Scholar

[34] V.A. Tischenko and V.M. Gun’ko: “Water electret relaxation at dispersed silica surfac”, Colloids Surface. A, Vol. 101, (1995), pp. 287–294. http://dx.doi.org/10.1016/0927-7757(95)03189-K10.1016/0927-7757(95)03189-KSearch in Google Scholar

[35] V.M. Gun’ko, V. V. Turov, R. Leboda, V. I. Zarko, J. Skubiszewska-Zięba, B. Charmas: “Adsorption, NMR and thermally stimulated depolarization current methods for comparative analysis of heterogeneous solid and soft materials”, Langmuir, Vol. 23, (2007), pp. 3184–3192. http://dx.doi.org/10.1021/la062648g10.1021/la062648gSearch in Google Scholar PubMed

[36] V.M. Gun’ko, V.V. Turov, A.V. Turov, V.I. Zarko, V.I. Gerda, V.V. Yanishpolskii, I.S. Berezovska, V.A. Tertykh: “Structural characteristics of ordered mesoporous silicas and properties of adsorbed pure water and water mixed with benzene or chloroform”, Cent. Eur. J. Chem., Vol. 5, (2007), pp. 420–454. http://dx.doi.org/10.2478/s11532-007-0010-310.2478/s11532-007-0010-3Search in Google Scholar

[37] V.V. Turov, N.P. Galagan, I.V. Gritsenko and A.A. Chuiko: “Hydration of erythrocyte membranes and their interaction with highly disperse silica”, Biopolymer. Cell., Vol. 22, (2006), pp. 56–62 (in Russian). Search in Google Scholar

[38] P. Bennekou, T.L. Barksmann, P. Christophersen and B.I. Kristensen: “The human red cell voltage-dependent cation channel. Part III: Distribution homogeneity and pH dependence”, Blood Cell. Mol. Dis., Vol. 36, (2006), pp. 10–14. http://dx.doi.org/10.1016/j.bcmd.2005.10.00410.1016/j.bcmd.2005.10.004Search in Google Scholar PubMed

[39] M.-P. Sauviat, R. Boydron-Le Garrec, J.-B. Masson, R.L. Lewis, J.-P. Vernoux, J. Molgó, D. Laurent and E. Benoit: “Mechanisms involved in the swelling of erythrocytes caused by Pacific and Caribbean ciguatoxins”, Blood Cell. Mol. Dis., Vol. 36, (2006), pp. 1–9. http://dx.doi.org/10.1016/j.bcmd.2005.10.00710.1016/j.bcmd.2005.10.007Search in Google Scholar PubMed

[40] A. Goto, T. Hondoh and S. Mae: “The electron density distribution in ice Ih determined by single-crystal x-ray diffractometry”, J. Chem. Phys., Vol. 93, (1990), pp. 1412–1417 http://dx.doi.org/10.1063/1.45915010.1063/1.459150Search in Google Scholar

[41] A.A. Rugal, V.M. Gun’ko, V.N. Barvinchenko, V.V. Turov, T.V. Semeshkina and V.I. Zarko: “Interaction of fibrinogen with nanosilica”, Cent. Eur. J. Chem., Vol. 5(1), (2007), pp. 32–54. http://dx.doi.org/10.2478/s11532-006-0067-410.2478/s11532-006-0067-4Search in Google Scholar

[42] P.I. Haris, D. Chapman and G. Benga: “A Fourier-transform infrared spectroscopic investigation of the hydrogen-deuterium exchange and secondary structure of the 28-kDa channel-forming integral membrane protein (CHIP28)”, Eur. J. Biochem., Vol. 233(2), (1995), pp. 659–664. http://dx.doi.org/10.1111/j.1432-1033.1995.659_2.x10.1111/j.1432-1033.1995.659_2.xSearch in Google Scholar PubMed

Published Online: 2007-9-12
Published in Print: 2007-12-1

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