A first-derivative UV spectrophotometric method, with or without the subtraction technique, was developed for the determination of biapenem in pharmaceutical dosage form in the presence of its degradation products. The method was based on the measurement of first-derivative amplitudes at zero crossing point (λ = 312 nm) and the peak-to-zero technique and validated with regard to linearity, limits of detection and quantitation, selectivity and precision. The observed rate constants for the degradation of biapenem were comparable to those obtained in the stability-indicating HPLC method.
 M. Hikida, M. Mori, T. Kitta, Chemother. 42, 101 (1994) Search in Google Scholar
 I. Kayo, I. Kazuro, M. Norifumi, K. Keiko, U. Nami, O. Hiroki et al., J. Chrom. B 867, 20, (2008) http://dx.doi.org/10.1016/j.jchromb.2008.03.00110.1016/j.jchromb.2008.03.001Search in Google Scholar PubMed
 M. Zając, J. Cielecka-Piontek, A. Jelińska, Anal. Chem. 51, 761 (2006) Search in Google Scholar
 Validation of analytical procedures, Proceeding of the International Conference of Harmonisation (ICH), Commission of the European Communities (2009) Search in Google Scholar
 E. Pawełczyk, T. Hermann, The Fundamentals of stability drugs (PZWL, Warsaw, 2006) (in Polish) Search in Google Scholar
 J. Cielecka-Piontek, A Lunzer, A. Krause, P. Zalewski, A. Jelińska, Acta Chromatographica 2010 (in press) Search in Google Scholar
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