Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access December 16, 2010

Meldola’s Blue — doped titania sol-gel sensor for NADH determination

  • Juliusz Adamski EMAIL logo and Jolanta Kochana
From the journal Open Chemistry


Titania layers obtained by a sol-gel technique doped with redox mediator, Meldola’s Blue, were employed for construction of a new NADH senor. Optimization of preparation process as well as experimental conditions affecting the response of the sensor were examined. Under optimal conditions NADH could be determined in the wide linear range from 90 to 2300 µM with detection limit 12 µM and a high sensitivity 12.5 nA µM−1. The usefulness of developed sensor was preliminarily checked in determination of NADH forming during enzymatic oxidation of ethanol catalyzed by alcohol dehydrogenase (ADH).

[1] L. Zhu, J. Zhai, R. Yang, Ch. Tian, L. Guo, Biosens. Bioelectron. 22, 2768 (2007) in Google Scholar PubMed

[2] H. J. Schmidt, Sol-Gel Sci. Techn. 40, 115 (2006) in Google Scholar

[3] J. Kochana, A. Gala, A. Parczewski, J. Adamski, Anal. Bioanal. Chem. 391, 1275 (2008) in Google Scholar PubMed

[4] T. Tsujimoto, T. Yabutani, A. Sano, Y. Tani, H. Murotani, Y. Mishima, K. Maruyama, M. Yasuzawa, J. Motonaka, Anal. Sci. 23, 59 (2007) in Google Scholar PubMed

[5] Y.D. Tanimoto de Albuquerqu, L.F. Ferreira, Anal. Chim. Acta 596, 210 (2007) in Google Scholar PubMed

[6] P. Du, S. Liu, P. Wu, Ch. Cai, Electrochim. Acta 53, 1811 (2007) in Google Scholar

[7] A.S. Santos, N. Duran, L.T. Kubota, Electroanalysis 17, 1103 (2005) in Google Scholar

[8] C.R. Raj, S. Behera, Biosens. Bioelectron. 21, 949 (2005) in Google Scholar PubMed

[9] J. Kochana, P. Nowak, A. Jarosz-Wilkołazka, M. Bieroń, Microchem. J. 89, 171 (2008) in Google Scholar

[10] R. Vaidya, E. Wilkins, Med. Eng. Phys. 16(5), 416 (1994) in Google Scholar PubMed

[11] S.A. Kumar, S.-M. Chen, Anal. Chim. Acta 592, 36 (2007) in Google Scholar PubMed

[12] C.M. Maroneze, L.T. Arenas, R.S.C. Luz, E.V. Benvenutti, R. Landers, Y. Gushikem, Electrochim. Acta 53, 4167 (2008) in Google Scholar

[13] A.S. Santos, L. Gorton, L.T. Kubota, Electroanalysis 14, 805 (2002)<805::AID-ELAN805>3.0.CO;2-P10.1002/1521-4109(200206)14:12<805::AID-ELAN805>3.0.CO;2-PSearch in Google Scholar

[14] S. Sampath, O. Lev, J. Electroanal. Chem. 446, 57 (1998) in Google Scholar

[15] B. Prieto-Simon, E. Fabregas, Biosens. Bioelectron. 19, 1131 (2004) in Google Scholar

[16] B. Prieto-Simon, J. Macanas, M. Munoz, E. Fabregas, Talanta 71, 2102 (2007) in Google Scholar

[17] A. Vasilescu, S. Andreescu, C. Bala, C.S. Litescu, T. Noguer, J.-L. Marty, Biosens. Bioelectron. 18, 781 (2003) in Google Scholar

[18] A. Arvinte, A.M. Sesay, V. Virtanen, C. Bala, Electroanalysis 20, 2355 (2008) in Google Scholar

Published Online: 2010-12-16
Published in Print: 2011-2-1

© 2011 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 1.3.2024 from
Scroll to top button