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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access July 21, 2012

Isocratic liquid chromatographic determination of three paraben preservatives in hygiene wipes using a reversed phase core-shell narrow-bore column

Paraskevas Tzanavaras, Theano Karakosta, Pantelis Rigas, Demetrius Themelis and Anastasia Zotou
From the journal Open Chemistry

Abstract

The first HPLC method for the separation of three paraben preservatives (methyl-, ethyl- and propyl parabens) using a core-shell analytical column is reported in this study. The separation was completed in less than 8 min at a low flow rate of 0.4 mL min−1 and an isocratic mobile phase containing 20% acetonitrile as organic modifier. The backpressure was HPLC equipment. The proposed analytical procedure was validated for linearity (0.5–20 µg L−1), limits of detection (15–43 µg L−1) and quantification (50–142 µg L−1), selectivity, within day (1.3–1.5%) and day-to-day (3.4–4.6%) precision and accuracy. The proposed method has been applied to the determination of the selected paraben preservatives in commercially available hygiene wipes. The mean percent recoveries were found to be in the range of 98.0–98.4%.

[1] S. Fekete, E. Olah, J. Fekete, J. Chromatogr. A 1228, 57 (2012) http://dx.doi.org/10.1016/j.chroma.2011.09.05010.1016/j.chroma.2011.09.050Search in Google Scholar PubMed

[2] A. Cavazzini, F. Gritti, K. Kaczmarski, N. Marchetti, G. Guiochon, Anal. Chem. 79, 5972 (2007) http://dx.doi.org/10.1021/ac070571a10.1021/ac070571aSearch in Google Scholar PubMed

[3] F. Gritti, I. Leonardis, J. Abia, G. Guiochon, J. Chromatogr. A 1217, 3819 (2010) http://dx.doi.org/10.1016/j.chroma.2010.04.02610.1016/j.chroma.2010.04.026Search in Google Scholar PubMed

[4] U.S. Food and Drug Administration (2009) [URL: http://www.fda.gov/Cosmetics/ProductandIngredientSafety/SelectedCosmeticIngredients/ucm128042.htm] (accessed January 2012) Search in Google Scholar

[5] P.D. Darbre, A. Aljarrah, W.R. Miller, N.G. Coldham, M.J. Sauer, G.S. Pope, J. Appl. Toxicol. 24, 5 (2004) http://dx.doi.org/10.1002/jat.95810.1002/jat.958Search in Google Scholar PubMed

[6] F.A. Andersen, Int. J. Toxicol. 27(SUPPL 4), 1 (2008) Search in Google Scholar

[7] J.F. Howlett, Food Add. Contam. 9, 607 (1992) http://dx.doi.org/10.1080/0265203920937411410.1080/02652039209374114Search in Google Scholar PubMed

[8] G.A. Shabir, Indian J. Pharm. Sci. 72, 421 (2010) http://dx.doi.org/10.4103/0250-474X.7390610.4103/0250-474X.73906Search in Google Scholar PubMed PubMed Central

[9] A. Zotou, I. Sakla, P.D. Tzanavaras, J. Pharm. Biomed. Anal. 53, 785 (2010) http://dx.doi.org/10.1016/j.jpba.2010.05.01810.1016/j.jpba.2010.05.018Search in Google Scholar PubMed

[10] M.M. Mincea, I.R. Lupşa, D.F. Cinghiţå, C.V. Radovan, I. Talpos, V. Ostafe, J. Serbian Chem. Soc. 74, 669 (2009) http://dx.doi.org/10.2298/JSC0906669M10.2298/JSC0906669MSearch in Google Scholar

[11] G. Shanmugam, B.R. Ramaswamy, V. Radhakrishnan, H. Tao, Microchem. J. 96, 391 (2010) http://dx.doi.org/10.1016/j.microc.2010.07.00510.1016/j.microc.2010.07.005Search in Google Scholar

[12] J. Šafra, M. Pospíšilová, J. Pharm. Biomed. Anal. 48, 452 (2008) http://dx.doi.org/10.1016/j.jpba.2008.01.03010.1016/j.jpba.2008.01.030Search in Google Scholar PubMed

[13] Y. Yang, C.C. Hodges, LC-GC North Amer. 23, 31 (2005) Search in Google Scholar

Published Online: 2012-7-21
Published in Print: 2012-10-1

© 2012 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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