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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access November 29, 2012

Quantification of sunscreen 2-phenylbenzimidazole-5-sulfonic acid in cosmetic products and water samples by HPTLC/densitometry with fluorescent detection

Anna Sobanska EMAIL logo , Karolina Derecka and Jaroslaw Pyzowski
From the journal Open Chemistry


The water-soluble sunscreen 2-phenylbenzimidazole-5-sulfonic acid (PBS) was quantified in a sun-care product and water samples by thin layer chromatography followed by densitometric scanning in fluorescence mode (cut-off filter 370 nm, wavelength of excitation — 300 nm). Normal phase TLC was performed on silica gel 60 as stationary phase. Mobile phase used was ethyl acetate-ethanol-water 70:35:30 (v/v/v). The limit of detection (LOD) was 0.0004 μg spot-1, and the limit of quantification (LOQ) was — 0.001 µg spot−1 without any sample pre-concentration.

[1] A. Salvador, A. Chisvert, Anal. Chim. Acta 537, 1 (2005) in Google Scholar

[2] A. Chisvert, M.T. Vidal, A. Salvador, Anal. Chim. Acta 464, 295 (2002) in Google Scholar

[3] J.S. Azevedo, N.S. Viana Jr, C.D. Vianna Soares, Il Farmaco 54, 573 (1999) in Google Scholar

[4] P.G. Pietta, A. Bruno, P.L. Mauri, C. Gardana, R. Maffei-Facino, M. Carini, J. Pharm. Biomed. Anal. 13, 229 (1995) in Google Scholar

[5] C.W. Kampfl, T. Leitner, J. Sep. Sci. 26, 1259 (2003) in Google Scholar

[6] C.W. Klampf, T. Leitner, E.F. Hilder, Electrophoresis 23, 2424 (2002)<2424::AID-ELPS2424>3.0.CO;2-U10.1002/1522-2683(200208)23:15<2424::AID-ELPS2424>3.0.CO;2-USearch in Google Scholar

[7] Z. Leon, A. Balaguer, A. Chisvert, A. Salvador, M. Herraez, O. Diez, Anal. Bioanal. Chem. 391, 859 (2008) in Google Scholar

[8] M.T. Vidal, A. Chisvert, A. Salvador, Talanta 59, 591 (2003) in Google Scholar

[9] G. Yener, J. Hadgraft, W.J. Pugh, Acta Pharm. Turcica 40, 27 (1998) Search in Google Scholar

[10] R. Rodil, J.B. Quintana, P. Lopez-Mahia, S. Muniategui-Lorenzo, D. Prada-Rodriguez, Anal. Chem. 80, 1307 (2008) in Google Scholar

[11] A. Nieto, F. Borrull, R.M. Marce, E. Pocurull, J. Chromatogr. A 1216, 5619 (2009) in Google Scholar

[12] A.W. Sobanska, J.Pyzowski, J. Planar Chromatogr. 25, 344 (2012) in Google Scholar

[13] J.J. Inbaraj, P. Bilski, C.F. Chignell, Photochem. Photobiol. 75, 107 (2002)<0107:PAPSOP>2.0.CO;210.1562/0031-8655(2002)075<0107:PAPSOP>2.0.CO;2Search in Google Scholar

[14] A.W. Sobanska, E. Brzezinska, Acta Pol. Pharm. 69, 791 (2012) Search in Google Scholar

[15] K. Ferenczi-Fodor, B. Renger, Z. Vegh, J. Planar Chromatogr. 23, 173 (2010) in Google Scholar

[16] ICH Harmonized Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology, Q2 (R1) (Geneva, 2005) Search in Google Scholar

[17] P. Konieczko, J. Namiesnik (Eds.), Ocena i kontrola jakości wyników pomiarów analitycznych (Evaluation and quality control of analytical results) (Wydawnictwo Naukowo-Techniczne, Warszawa 2007) pp. 269–288 (In Polish) Search in Google Scholar

Published Online: 2012-11-29
Published in Print: 2013-2-1

© 2013 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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