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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access November 29, 2012

Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract

Dragoljub Miladinović EMAIL logo , Budimir Ilić , Stevo Najman , Olga Cvetković , Aleksandra Šajnović , Marija Miladinović and Nikola Nikolić
From the journal Open Chemistry


The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.

[1] A. Pitzschke, C. Forzani, H. Hirt, Antioxid. Redox Sign. 8, 1757 (2006) in Google Scholar

[2] O. Ozkur, F. Ozdemir, M. Bor, I. Turkan, Environ. Exp. Bot. 66, 487 (2009) in Google Scholar

[3] Z. Noreen, M. Ashraf, Environ. Exp. Bot. 67, 395 (2009) in Google Scholar

[4] B. Márquez-García, F. Córdoba, Environ. Exp. Bot. 68, 58 (2010) in Google Scholar

[5] D. Podlech, Proc. Roy. Soc. Edinb. B. 89, 37 (1986) 10.1017/S0269727000008885Search in Google Scholar

[6] S.D. Lindblom et al., Environ. Exp. Bot. (in press), DOI:10.1016/j.envexpbot.2011.12.011 10.1016/j.envexpbot.2011.12.011Search in Google Scholar

[7] N. Diklić, In: M. Josifović (Ed.), Flora of Serbia (Serbian academy of Science and Art, Belgrade, 1974) 274 Search in Google Scholar

[8] D.L. Miladinović, B.S. Ilić, V.N. Milosavljević, Hem. Ind. 65, 323 (2011) in Google Scholar

[9] A. Kabata-Pendias, Trace elements in soils and plants, 4th edition (CRC Press, New York, 2011) 10.1201/b10158Search in Google Scholar

[10] Z.A. Placer, L.L. Cushman, B.C. Johnson, Anal. Biochem. 16, 359 (1966) in Google Scholar

[11] H.P. Misra, I. Fridovich, J. Biol. Chem. 247, 3170 (1972) 10.1016/S0021-9258(19)45228-9Search in Google Scholar

[12] K.H. Cheeseman, A. Beavis, H. Esterbauer, Biochem. J. 252, 649 (1988) 10.1042/bj2520649Search in Google Scholar

[13] L.M. Simon, Z. Fatrai, D.E. Jonas, B. Matkovics, Biochem. Physiol. Pflanzen 166, 387 (1974) 10.1016/S0015-3796(17)30073-2Search in Google Scholar

[14] B. Matkovics, R. Novak, H.D. Hanh, Comp. Biochem. Phys. 56, 397 (1977) 10.1016/0305-0491(77)90238-3Search in Google Scholar

[15] D.T.Y. Chiu, F.H. Stults, A.L. Tappel, Biochim. Biophys. Acta 445, 558 (1976) in Google Scholar

[16] M.M. Bradford, Anal. Biochem. 72, 248 (1976) in Google Scholar

[17] I. Jantan, N. Hikmah Harun, A. Wira Septama, S. Murad, M.A. Mesaik, J. Nat. Med. 65, 400 (2011) in Google Scholar PubMed

[18] W.S. Koko, M.A. Mesaik, S. Yousaf, M. Galal, M.I. Choudhary, J. Ethnopharmacol. 118, 26 (2008) in Google Scholar PubMed

[19] V. Katsares et al., Lab. Med. 40, 557 (2009) in Google Scholar

[20] D. Miladinović, Antioxidant system of plants (University of Niš, Niš, 2011) Search in Google Scholar

[21] S. Najman, Č. Radojičić, A. Dujić, Period. Biol. 88, 256 (1986) Search in Google Scholar

[22] R. Khanna-Chopra, D.S. Selote, Environ. Exp. Bot. 60, 276 (2007) in Google Scholar

[23] Y. Liang et al., Environ. Exp. Bot. 64, 286 (2008) in Google Scholar

[24] D. Miladinović, L. Miladinović, S. Najman, J. Serb. Chem. Soc. 76, 505 (2011) in Google Scholar

[25] L. Simova-Stoilova, K. Demirevska, T. Petrova, N. Tsenov, U. Feller, Plant Soil Environ. 54, 529 (2008) 10.17221/427-PSESearch in Google Scholar

[26] P.A. Leand, MSc thesis, Texas Tech University (Lubbock, USA, 1986) Search in Google Scholar

[27] J. Bogdanović, N. Milosavić, R. Prodanović, T. Dučić, K. Radotić, Biochem. Syst. Ecol. 35, 263 (2007) in Google Scholar

[28] I. Öncel, E. Yurdakulol, Y. Keleş, L. Kurt, A. Yildiz, Acta Oecol. 26, 211 (2004) in Google Scholar

[29] J.V. Anderson, B.I. Chevone, J.L. Hess, Plant Physiol. 98, 501 (1992) in Google Scholar PubMed PubMed Central

[30] X. Wang, G. Zhao, H. Gu, Afr. J. Biotechnol. 8, 5773 (2009) Search in Google Scholar

[31] S.M. Bian, Y.W. Jiang, Sci. Hortic. 120, 264 (2009) in Google Scholar

[32] Y. Tan, Z. Liang, H. Shao, F. Du, Colloid. Surface. B. 49, 60 (2006) in Google Scholar PubMed

[33] H. Kai, L. Zongsuo, D. Qimei, Acta Agr. Bor.-Occident. Sinica 5, 100 (2010) Search in Google Scholar

[34] N. Navrot et al., Plant Physiol. 142, 1364 (2006) in Google Scholar PubMed PubMed Central

[35] Y. Miao et al., Plant Cell 18, 2749 (2006) in Google Scholar PubMed PubMed Central

[36] D.I. Štajner, B.M. Popović, P. BoŽa, A. Kapor, Phytother. Res. 23, 1006 (2009) in Google Scholar PubMed

[37] L. Saleh, C. Plieth, Plant Methods 5, 1 (2009) in Google Scholar

[38] S.R. Georgetti, R. Casagrande, V.M. Di Mambro, A.E.C.S. Azzolini, M.J.V. Fonseca, AAPS PharmSci 5, 1 (2003) in Google Scholar

[39] M. Kawagoe, K. Nakagawa, Toxicol. Lett. 114, 189 (2000) in Google Scholar

[40] J. Asgarpanah, S.M. Motamed, A. Farzaneh, B. Ghanizadeh, S. Tomraee, Afr. J. Biotechnol. 10, 19176 (2011) 10.5897/AJB11.3073Search in Google Scholar

[41] J.G. Souza et al., Z. Naturforsch. C. 62, 339 (2007) Search in Google Scholar

[42] B. Gonzalez Flecha, S. Llesuy, A. Boveris, Free Radical Bio. Med. 10, 93 (1991) in Google Scholar

[43] C. Desmarchelier, J. Coussio, G. Ciccia, Braz. J. Med. Biol. Res. 31, 1163 (1998) in Google Scholar

[44] D.L. Miladinović et al., Anal. Bioanal. Chem. 403, 1007 (2012) in Google Scholar PubMed

Published Online: 2012-11-29
Published in Print: 2013-2-1

© 2013 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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