Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access July 19, 2013

In vitro biological activity comparison of some hydroxyapatite-based composite materials using simulated body fluid

  • Melinda Czikó EMAIL logo , Erzsébet-Sára Bogya , Réka Barabás , Liliana Bizo and Răzvan Stefan
From the journal Open Chemistry


Hydroxyapatite composites are the main biomaterials used for metal implant coatings. Their in vitro study is very important. That is why their behavior was monitored in simulated body fluid (SBF), which is a solution with ion concentrations and pH value similar to those of human blood plasma. Silica, chitosan and gelatin-doped hydroxyapatite-based biomaterials were studied in SBF; the samples were characterized pre-, during and post-SBF immersion using infra-red, scanning and transmission electron spectroscopy and X-ray diffraction methods. The solubility of materials in SBF was determined, and the variation of Ca2+ and phosphorus concentration was also recorded during SBF experiments. The results were compared and their in vitro biological activity was determined.

[1] L.D. Silvio, M. Dalby, W. Bonfield, J. Mater. Sci: Mater. Med. 9, 12 (1998) in Google Scholar

[2] A.M. Ambrosio, J.S. Sahota, Y. Khan, C.T. Laurencin, J. Biomed. Mater. Res. 58, 3 (2001)<295::AID-JBM1020>3.0.CO;2-810.1002/1097-4636(2001)58:3<295::AID-JBM1020>3.0.CO;2-8Search in Google Scholar

[3] A. Afshar, M. Ghorbani, N. Ehsani, M. R. Saeri, C.C. Sorrell, Intern. J. Eng. Trans. B: Appl. 16, 4 (2003 Search in Google Scholar

[4] M. Vallet-Regi, D. Arcos, J. Mater. Chem. 15, 15 (2005) in Google Scholar

[5] P. Ducheyne, Q. Qui, Biomaterials 20, 23 (1999) in Google Scholar

[6] X. Fan, J. Chen, J-P. Zou, Q. Wan, Z-C. Zhou, J-M. Ruan, Trans. Nonferrous Met. Soc. China 19, 2 (2009) in Google Scholar

[7] X. Zhao, L. Yang, Y. Zuo, J. Xiong, Chin. J. Chem. Eng. 17, 4 (2009) 10.1016/S1004-9541(08)60261-XSearch in Google Scholar

[8] X. Pang, I. Zhitomirsky:, Mater. Charact. 58, 4 (2007) 10.1016/j.matchar.2006.05.011Search in Google Scholar

[9] Q. Hu, B. Li, M. Wang, J. Shen, Biomaterials 25, 5 (2004) in Google Scholar

[10] A.A. Haroun, V. Migonney:, Int. J. Biol. Macromol. 46, 3 (2010) in Google Scholar

[11] W.W. Thein-Han, R.D.K. Misra, Acta Biomater. 5, 4 (2009) 10.1016/j.actbio.2008.11.025Search in Google Scholar

[12] K. Zhang, M. Zhao, L. Cai, Z-k. Wang, Y-f. Sun, Q-l. Hu, Chin. J. Polym. Sci. 28, 4 (2010) 10.1007/s10118-010-9087-9Search in Google Scholar

[13] O.C. Wilson Jr., J.R. Hull, Mater. Sci. Eng., C 28, 3 (2008) 10.1016/j.msec.2007.04.005Search in Google Scholar

[14] A.E. Porter, N. Patel, J.N. Skepper, S.M. Best, W. Bonfield, Biomaterials 24, 25 (2003) in Google Scholar

[15] N. Patel et al., J. Mater. Sci. Mater. Med. 13, 12 (2002) in Google Scholar

[16] J.H. Ko et al., Macromol. Res. 18, 2 (2010) in Google Scholar

[17] S. Teng, L. Chen, Y. Guo, J. Shi, J. Inorg. Biochem. 101, 4 (2007) in Google Scholar

[18] T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi, T. Yamamuro, J. Biomed. Mater. Res. 24, 6 (1990) 10.1002/jbm.820240607Search in Google Scholar

[19] H. Maeda, T. Kasuga, M. Nogami, J. Eur. Ceram. Soc. 24, 7 (2004) in Google Scholar

[20] S.B. Cho, F. Miyaji, T. Kokubo, K. Nakanishi, N. Soga, T. Nakamura, J. Mater. Sci. Mater. Med. 9, 5 (1998) in Google Scholar

[21] T. Kokubo, H. Takadama, Biomaterials 27, 15 (2006) in Google Scholar PubMed

[22] T. Kokubo, S. Ito, M. Shigematsu, S. Sanka, T. Yamamuro, J. Mater. Sci. 22, 11 (1987) in Google Scholar

[23] J.A. Juhasz, S.M. Best, A.D. Auffret, W. Bonfield, J. Mater. Sci. Mater. Med. 19, 4 (2008) in Google Scholar PubMed

[24] J. Garnett, P. Dieppe, Biochem. J. 266, 3 (1990) Search in Google Scholar

[25] S.L. Teitelbaum, Science 289, 5484 (2000) in Google Scholar PubMed

[26] M. Bohner, J. Lemaitre, Biomaterials 30, 12 (2009) 10.1016/j.biomaterials.2009.01.008Search in Google Scholar PubMed

[27] M. Jarcho, C.H. Bolen, M.B. Thomas, J. Bobick, J.F. Kay, R.H. Doremus, J. Mater. Sci. 11, 11 (1976) in Google Scholar

[28] Al-C. Dancu, R. Barabas, E.S. Bogya, Cent. Eur. J. Chem. 9, 4 (2011) in Google Scholar

[29] E.S. Bogya, R. Barabás, V.R. Dejeu, Al. Csavdari, I. Baldea, Chem. Pap. 63, 5 (2009) in Google Scholar

[30] R. Barabás, M. Cziko, I. Dékány, L. Bizo, E.S. Bogya, Chem. Pap. (in press) Search in Google Scholar

[31] A. Ślósarczyk, Z. Paszkiewicz, C. Paluszkiewicz, J. Mol. Struct. 744 (2005) 10.1016/j.molstruc.2004.11.078Search in Google Scholar

[32] P.N. Chavan, M.M. Bahir, R.U. Mene, M.P. Mahabole, R.S. Khairnar, Mater. Sci. Eng., B 168, 1 (2010) in Google Scholar

[33] L. Wang, C. Li, Carbohydr. Polym. 68, 4 (2007) Search in Google Scholar

[34] L. Kong, Y. Gao, G. Lu, Y. Gong, N. Zhao, X. Zhang, Eur. Polym. J. 42, 12 (2006) in Google Scholar

[35] H.E. Feki, J.M. Savariault, A.B. Salah, M. Jemal, Solid State Sci. 2, 5 (2000) 10.1016/S1293-2558(00)01059-1Search in Google Scholar

[36] L-T. Zhang, W-F. Xie, Y-D. Wu, H. Xing, A-W. Li, W. Zheng, Y-S. Zhang, Chinese Phys. Lett. 20, 8 (2003) in Google Scholar

[37] M.S. Sadjadi, H.R. Ebrahimi, M. Meskinfam, K. Zare, Mater. Chem. Phys. 130, 1 (2011) in Google Scholar

[38] N. Davidenko, R.G. Carrodeguas, C. Peniche, Y. Solís, R.E. Cameron, Acta Biomater. 6, 2 (2010) 10.1016/j.actbio.2009.07.029Search in Google Scholar PubMed

[39] P. Jongwattanapisan et al., Mater. Sci. Eng., C 31, 2 (2011) in Google Scholar

[40] T-J.M. Luo, C-C. Ko, C-K. Chiu, J. Llyod, U. Huh, J. Sol-Gel Sci. Technol. 53, 2 (2010) in Google Scholar PubMed PubMed Central

[41] K.R. Mohamed, Z.M. El-Rashidy, A.A. Salama, Ceram. Int. 37, 8 (2011) in Google Scholar

[42] S. Gao, R. Sun, Z. Wei, H. Zhao, H. Li, F. Hu, J. Fluorine Chem. 130, 6 (2009) in Google Scholar

[43] R. Sun, M. Li, Y. Lu, A. Wang, Mater. Char. 56, 3 (2006) in Google Scholar

[44] X. Lu, Y. Leng, Biomaterials 26, 10 (2005) 10.1016/j.biomaterials.2004.05.034Search in Google Scholar PubMed

[45] J. Oliva, J. Cama, J.L. Cortina, C. Ayora, J. De Pablo, J. Hazard. Mater. 213 (2012) 10.1016/j.jhazmat.2012.01.027Search in Google Scholar PubMed

[46] H.M. Kim, T. Himeno, T. Kokubo, T. Nakamura, Biomaterials 26, 21 (2005) 10.1016/j.biomaterials.2004.11.022Search in Google Scholar PubMed

Published Online: 2013-7-19
Published in Print: 2013-10-1

© 2013 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 10.12.2023 from
Scroll to top button