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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access April 30, 2014

In vitro hemocompatibility and corrosion behavior of new Zr-binary alloys in whole human blood

  • Georgeta Totea EMAIL logo , Daniela Ionita , Ioana Demetrescu and Mihaela Mitache
From the journal Open Chemistry


The aim of this study is to evaluate the accuracy of three binary alloys’ composition, and their biocompatibility. Depending on the intended use of the medical devices made from these materials, dynamic or static tests should be performed. We have chosen static tests as we thought they may be used as knee or hip replacement, and not as cardiac valves.

Three binary alloys ( Zr10Nb, Zr2.5Nb and Zr12Ta) were obtained from high purity powders (>99.9%), using an induction furnace first, and an electric arc furnace for a perfect homogenization. Their final composition was verified with a XRF analyzer-INNOV-X.

Hemolysis tests can determine the degree of red blood cells lysis and the release of hemoglobin. The released hemoglobin quantity was extremely small, under 2%, in all cases, and the coagulation tests showed no risk for thrombosis. The electrochemical behavior was also studied in biological fluid, human female serum, and showed a low corrosion rate.

The obtained alloys do not cause hemolysis, so they are hemocompatible with all blood types.

[1] S. Krajewski & al., Acta Biomater. 9, 7460 (2013) in Google Scholar

[2] A. Gomez Sanchez, W. Schreiner, G. Duffóc, S. Ceré, Appl. Surf. Sci. 257, 6397 (2011) in Google Scholar

[3] ISO 10993-4:2002/Amd 1:2006 Biological evaluation of medical devices Part 4: Selection of tests for interactions with blond (International Organization for Standardization, Geneva, Switzerland, 2009) Search in Google Scholar

[4] S. Logothetidis, Diamond Relat. Mater. 16, 1847 (2007) in Google Scholar

[5] L.S. Bolen, O. Svendsen, Regulatory guidelines for biocompatibility safety testing, Medical plastics and biomaterials: materials technology for medical products 4, 16 (1997) Search in Google Scholar

[6] V. Han, K. Serrano, D.V. Devine, Vox Sang. 98, 116 (2010) in Google Scholar

[7] P. Balasubramaniam, A. Malathi, J. Postgrad. Med. 38, 8 (1992) Search in Google Scholar

[8] ASTM F756-00, Standard Practice for Assessment of Hemolytic Properties of Materials (ASTM International, West Conshohocken, PA, USA, 2004) Search in Google Scholar

[9] W. Van Oeveren, P. Schoen, C.A. Maijers, S.H. Monnink, A.J. Van Boven, Progress in Biomedical Research. 4, 17 (1999) Search in Google Scholar

[10] F. Date, C. Wagner, Hemostasis (Lothar Thomas’ Clinical Laboratory Diagnostics, Frankfurt, 1998) 602 Search in Google Scholar

[11] F. Fischbach, A Manual of Laboratory and Diagnostic Test, 8th edition (Lippincott, Philadelphia, 2009) 161 Search in Google Scholar

[12] C.M. Venturini, J.E. Kaplan, Semin. Thromb. Hemost. 18, 275 (1992) in Google Scholar

[13] C. Sperling, M. Fischer, M.F. Maitz, C. Werner, Biomaterials 30, 4447 (2009) in Google Scholar

[14] A. Mazare, M. Dilea, D. Ionita, I. Titorencu, V. Trusca, E. Vasile, Bioelectrochemistry 87, 124 (2012) in Google Scholar

[15] B.W. Buczynski, M.M. Kory, R.P. Steiner, T.A. Kittinger, R.D. Ramsier, Colloids Surf. B 30, 167 (2003) in Google Scholar

[16] Y. Tamada, Y. Ikada, J. Biomed. Mater. Res. 28, 783 (1994) in Google Scholar

[17] Y. Ikada, Biomaterials 15, 725 (1994) in Google Scholar

[18] P. Van der Valk, A.W.J. van Pelt, H.J. Busscher, H.P. de Jong, Ch.R.H. Wildevuur, J. Arends, J. Biomed. Mater. Res. 17, 807 (1983) in Google Scholar

[19] P.B. Van Wachem, T. Beguiling, J. Feijen, A. Bantjes, J.P. Detmers, W.G. van Aken, Biomaterials 6, 403 (1985) in Google Scholar

[20] E. Eisenbarth, D. Velten, M. Müller, R. Thull, J. Breme, Biomaterials 25, 5705 (2004) in Google Scholar PubMed

[21] F. Rosalbino, D. Macciò, A. Saccone, E. Angelini, S. Delfino, Mater. Corr. 63, 580 (2012) 10.1002/maco.201006012Search in Google Scholar

[22] Y.Z. Huang, D.J. Blackwood, Electroch. Acta 51, 1099 (2005) in Google Scholar

[23] D. Mareci, R. Chelariu, D.M. Gordin, G. Ungureanu, T. Gloriant, Acta Biomater. 5, 3625 (2009) in Google Scholar PubMed

Published Online: 2014-4-30
Published in Print: 2014-7-1

© 2014 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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