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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access December 25, 2011

Effect of Cordyceps sinensis on TIMP-1 secretion from mouse melanoma cell

Erika Kubo, Ayuko Sato, Noriko Yoshikawa, Satomi Kagota, Kazumasa Shinozuka and Kazuki Nakamura
From the journal Open Life Sciences

Abstract

Cordyceps sinensis is a Chinese medicinal fungus traditionally used in cancer treatments. In a previous study, we investigated the antimetastatic activity of Cordyceps sinensis (WECS) extract using liver metastatic model mice injected with B16-F0 mouse melanoma cells into the spleen. WECS reduced the number of metastatic nodules of B16-F0 cells in the liver of C57BL/6 mice, and significantly prolonged survival of the mice. Furthermore, we examined the effects of WECS on hepatocyte growth factor (HGF)-accelerated invasion of B16-F0 cells using a chemo-invasion assay in vitro. WECS was shown to significantly reduce HGF-accelerated B16-F0 cell invasion. In the present study, we investigated the effect of WECS on Tissue Inhibitor of Metalloproteinase (TIMP)-1 secretion from B16-F0 cells in order to identify clues to the mechanism underlying the anti-invasive action of WECS. As a result, WECS significantly increased the secretion of TIMP-1 from B16-F0 cells. Moreover, we investigated the effect of cordycepin (3′-deoxyadenosine), a component of WECS, on TIMP-1 secretion from B16-F0 cells to potentially identify the pharmacologically active ingredient in WECS extract. Cordycepin was shown to significantly accelerate the release of TIMP-1 from cells. These findings suggest that WECS exerts anti-invasive activity, in part by increasing TIMP-1 secretion from melanoma cells, and that cordycepin potentially functions as the effective component.

[1] Nakamura K., Yamaguchi Y., Kagota S., Shinozuka K., Kunitomo M., Activation of in vivo Kupffer cell function by oral administration of Cordyceps sinensis in rats, Jpn. J. Pharmacol., 1999, 79, 505–508 http://dx.doi.org/10.1254/jjp.79.50510.1254/jjp.79.505Search in Google Scholar

[2] Yamaguchi Y., Kagota S., Nakamura K., Shinozuka K., Kunitomo M., Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis, Phytother. Res., 2000, 14, 647–649 http://dx.doi.org/10.1002/1099-1573(200012)14:8<647::AID-PTR670>3.0.CO;2-W10.1002/1099-1573(200012)14:8<647::AID-PTR670>3.0.CO;2-WSearch in Google Scholar

[3] Yamaguchi Y., Kagota S., Nakamura K., Shinozuka K., Kunitomo M., Inhibitory effects of water extracts from fruiting bodies of cultured Cordyceps sinensis on raised serum lipid peroxide levels and aortic cholesterol deposition in atherosclerotic mice, Phytother. Res., 2000, 14, 650–652 http://dx.doi.org/10.1002/1099-1573(200012)14:8<650::AID-PTR675>3.0.CO;2-010.1002/1099-1573(200012)14:8<650::AID-PTR675>3.0.CO;2-0Search in Google Scholar

[4] Yoshikawa N., Nakamura K., Yamaguchi Y., Kagota S., Shinozuka K., Kunitomo M., Cordycepin and Cordyceps sinensis reduce the growth of human promyelocytic leukaemia cells through the Wnt signalling pathway, Clin. Exp. Pharmacol. Physiol., 2007, 34, S61–S63 http://dx.doi.org/10.1111/j.1440-1681.2007.04781.x10.1111/j.1440-1681.2007.04781.xSearch in Google Scholar

[5] Kubo E., Yoshikawa N., Kunitomo M., Kagota S., Shinozuka K., Nakamura K., Inhibitory effect of Cordyceps sinensis on experimental hepatic metastasis of melanoma by suppressing tumor cell invasion, Anticancer Res., 2010, 30, 3429–3434 Search in Google Scholar

[6] Fidler I.J., Selection of successive tumor lines for metastasis, Nature, 1973, 242, 148–149 10.1038/newbio242148a0Search in Google Scholar

[7] Poste G., Doll J., Hart R., Fidler I.J., In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties, Cancer Res., 1980, 40, 1636–1644 Search in Google Scholar

[8] Gomez D.E., Alonso D.F., Yoshiji H., Thorgeirsson U.P., Tissue inhibitors of metalloproteinases: structure, regulation and biological functions, Eur. J. Cell Biol., 1997, 74, 111–122 Search in Google Scholar

[9] Zeng H., Briske-Anderson M., Prolonged butyrate treatment inhibits the migration and invasion potential of HT1080tumor cells, J. Nutr., 2005, 135, 291–295 10.1093/jn/135.2.291Search in Google Scholar

[10] Zeng H., Briske-Anderson M., Idso J.P., Hunt C.D., The selenium metabolite methylselenol inhibits the migration and invasion potential of HT1080 tumor cells, J. Nutr., 2006, 136, 1528–1532 10.1093/jn/136.6.1528Search in Google Scholar

[11] Liu H.K., Wang Q., Li Y., Sun W.G., Liu J.R., Yang Y.M., et al., Inhibitory effects of Γ-tocotrienol on invasion and metastasis of human gastric adenocarcinoma SGC-7901 cells, J. Nutr. Biochem., 2010, 21, 206–213 http://dx.doi.org/10.1016/j.jnutbio.2008.11.00410.1016/j.jnutbio.2008.11.004Search in Google Scholar

[12] Luparello C., Avanzato G., Carella C., Pucci-Minafra I., Tissue inhibitor of metalloprotease (TIMP)-1 and proliferative behaviour of clonal breast cancer cells. Breast Cancer Res Treat, 54: 235–244, 1999. http://dx.doi.org/10.1023/A:100612112938210.1023/A:1006121129382Search in Google Scholar

[13] Schrohl A-S., Holten-Andersen M.N., Peters H.A., Look M.P., Meijer-van Gelder M.E., Klijn J.G.M., et al., Tumor tissue levels of tissue inhibitor of metalloproteinase-1 as a prognostic marker in primary breast cancer, Clin. Cancer Res., 2004, 10, 2289–2298 http://dx.doi.org/10.1158/1078-0432.CCR-03-036010.1158/1078-0432.CCR-03-0360Search in Google Scholar

[14] Egeblad M., Werb Z., New functions for the matrix metalloproteinases in cancer progression, Nat. Rev. Cancer, 2002, 2, 161–174 http://dx.doi.org/10.1038/nrc74510.1038/nrc745Search in Google Scholar PubMed

[15] Nakamura K., Konoha K., Yoshikawa N., Yamaguchi Y., Kagota S., Shinozuka K., et al., Effect of cordycepin (3−deoxyadenosine) on hematogenic lung metastatic model mice, In Vivo, 2005, 19, 137–142 Search in Google Scholar

[16] Yoshikawa N., Kunitomo M., Kagota S., Shinozuka K., Nakamura K., Inhibitory effect of cordycepin on hematogenic metastasis of B16-F1 mouse melanoma cells accelerated by adenosine-5′-diphosphate, Anticancer Res., 2009, 29, 3857–3860 Search in Google Scholar

Published Online: 2011-12-25
Published in Print: 2012-2-1

© 2011 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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