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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access August 3, 2012

Mammalian androgen stimulates photosynthesis in drought-stressed soybean

  • Anna Janeczko EMAIL logo , Maciej Kocurek and Izabela Marcińska
From the journal Open Life Sciences


The aim of the present studies was to assess the possibility of compensating the negative effects of drought stress on gaseous exchange and efficiency of photosystem II in soybean seedlings by application of the androgen — androstenedione. Androstenedione (0.25 mg dm−3) was applied via presowing seed soaking (12 h). Control seeds were untreated with steroid. Plants were cultured in pots. On the 12th day of growth, the plants were watered for the last time. Drought symptoms occurred during the next 10 days. On the 22nd day of growth, leaf gaseous exchange and PSII measurements were taken. Afterwards the plants were watered. Two days later measurements were taken again. Androstenedione improved the intensity of leaf net photosynthesis. The effect of androstenedione was manifested during the rehydration of plants that have undergone a period of drought. An increase in net photosynthesis intensity was accompanied by higher transpiration. Possible mechanisms of androstenedione action — effect on aquaporin functionality and membrane stability — are discussed. The significance of ethanol and DMSO (solvents of steroid) in experiments on the physiological activity of androstenedione is also considered.

[1] Mooradian A.D., Morley J.E., Korenman S.G., Biological actions of androgens, Endocr. Rev., 1987, 8, 1–28 in Google Scholar

[2] Schumacher M., Coirini H., Robert F., Guennoun R., El-Etr M., Genomic and membrane actions of progesterone: implications for reproductive physiology and behaviour, Behav. Brain Res., 1999, 105, 37–52 in Google Scholar

[3] Falkenstein E., Tillmann H.C., Christ M., Feuring M., Wehling M., Multiple actions of steroid hormones — a focus on rapid, nongenomic effects, Pharmacol. Rev., 2000, 52, 513–555 Search in Google Scholar

[4] Costet M.F., El Achouri M., Charlet M., Lanot R., Benveniste P., Hoffmann J.A., Ecdysteroid biosynthesis and embryonic development are disturbed in insects (Locusta migratoria) reared on plant diet (Triticum sativum) with a selectively modified sterol profile, Proc. Natl. Acad. Sci. USA, 1987, 84, 643–647 in Google Scholar

[5] Clouse S.D., Sasse J.M., Brassinosteroids: essential regulators of plant growth and Development, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1998, 49, 427–451 in Google Scholar

[6] Hayat S., Ahmad A., Brassinosteroids: a class of plant hormone, 1st Edition., Springer Science+Business Media B.V., 2011 10.1007/978-94-007-0189-2Search in Google Scholar

[7] Adler J.H., Grebenok R.J., Biosynthesis and distribution of insect-molting hormones in plants — a review, Lipids, 1995, 30, 257–262 in Google Scholar

[8] Simons R.G., Grinwich D.L., Immunoreactive detection of four mammalian steroids in plants, Can. J. Bot., 1989, 67, 288–96 in Google Scholar

[9] Iino M., Nomura N., Tamaki Y., Yamada Y., Yoneyama K., Takeuchi Y., et al. Progesterone: its occurrence in plants and involvement in plant growth, Phytochemistry, 2007, 68, 1664–1673 in Google Scholar

[10] Simerský R., Novak O., Morris D.A., Pouzar V., Strnad M., Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS, J. Plant Growth Regul., 2009, 28, 125–136 in Google Scholar

[11] Geuns J.M.C., Steroid hormones and plant growth and development, Phytochemistry, 1978, 17, 1–14 in Google Scholar

[12] Janeczko A., Skoczowski A., Mammalian sex hormones in plants, Folia Hist. Cytobiol., 2005, 43, 71–79 Search in Google Scholar

[13] Janeczko A., The presence and activity of progesterone in the plant kingdom, Steroids, 2012, 77, 169–173 in Google Scholar PubMed

[14] Erdal S., Alleviation of salt stress in wheat seedlings by mammalian sex hormones, J. Sci. Food Agric., 2011, 92, 1411–1416 in Google Scholar PubMed

[15] Erdal S., Dumlupinar R., Mammalian sex hormones stimulate antioxidant system and enhance growth of chickpea plants, Acta Physiol. Plant., 2011, 33, 1011–1017 in Google Scholar

[16] Janeczko A., Tóbiás I., Skoczowski A., Dubert F., Gullner G, Barna B., Progesterone attenuates both cell membrane damage and loss of photosynthetic efficiency caused by infection with Pseudomonas bacteria in Arabidopsis thaliana, Biol. Plant., 2012, In press Search in Google Scholar

[17] Rashid A., Harris D., Hollington P.A., Rafiq M., Improving the yield of mungbean (Vigna radiata) in the North West Frontier Province of Pakistan using on-farm seed priming, Exp. Agricult., 2004, 40, 233–244 in Google Scholar

[18] Rashid A., Hollington P.A., Harris D., Khan P., Onfarm seed priming for barley on normal, saline and saline-sodic soils in North West Frontier Province, Pakistan, Eur. J. Agron., 2006, 24, 276–281 in Google Scholar

[19] Gadallah M.A.A., Effects of indole-3-acetic acid and zinc on the growth, osmotic potential and soluble carbon and nitrogen components of soybean plants growing under water deficit, J. Arid Environ., 2000, 44, 451–467 in Google Scholar

[20] Khan W., Prithiviraj B., Smith D.L., Photosynthetic responses of corn and soybean to foliar application of salicylate, J. Plant Physiol., 2003, 160, 485–492 in Google Scholar PubMed

[21] Upreti K.K., Murti G.S.R., Effects of brassinosteroids on growth, nodulation, phytohormone content and nitrogenase activity in French bean under water stress, Biol. Plant., 2004, 48, 407–411 in Google Scholar

[22] Lu S., Su W., Li H., Guo Z., Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities, Plant Physiol. Bioch. 2009, 47, 132–138 in Google Scholar PubMed

[23] Chen W., Yao X., Cai K., Chen J., Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption, Biol. Trace Element Res., 2011, 142, 67–76 in Google Scholar PubMed

[24] Kley H.K., Deselaers T., Peerenboom H., Krüskemper H.L., Enhanced conversion of androstenedione to estrogens in obese males, J. Clin. Endocrinol. Metab., 1980, 51, 1128–32 in Google Scholar PubMed

[25] Milewich L., Whisenant M.G., Metabolism of androstenedione by human platelets: a source of potent androgens, J. Clin. Endocrinol. Metab., 1982, 54, 969–74 in Google Scholar PubMed

[26] Janeczko A., Filek W., Stimulation of generative development in partly vernalized winter wheat by animal sex hormones, Acta Physiol. Plant., 2002, 24, 291–295 in Google Scholar

[27] Janeczko A., Filek W., Biesaga-Kościelniak J., Marcińska I., Janeczko Z., The influence of animal sex hormones on the induction of flowering in Arabidopsis thaliana: comparison with the effect of 24-epibrassinolide, Plant Cell Tiss. Org. Cult., 2003, 72, 147–151 in Google Scholar

[28] Janeczko A., The significance of ethanol as a hormone solvent in experiments on the physiological activity of brassinosteroids, In: Hayat S., Ahmad A. (Eds.), Brassinosteroids: a class of plant hormone, 1st ed., Springer Science+Business Media B.V., 2011 Search in Google Scholar

[29] Strasser R.J., Srivatava A., Tsimilli-Michael M., The fluorescence transient as a tool to characterize and screen photosynthetics samples, In: Yunus M., Pathre U., Mohaty P. (Eds.), Probing photosynthesis: mechanism, regulation and adaptation, Taylor and Francis, London, 2000 Search in Google Scholar

[30] Eisenbarth D.A., Weig A.R. Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus communis L. seedlings, J. Exp. Bot. 2005, 56, 1831–1842 in Google Scholar PubMed

[31] Chamount F., Moshelion M., Daniels M.J., Regulation of plant aquaporin activity, Biol. Cell, 2005, 97, 749–764 in Google Scholar PubMed

[32] Porcel R., Aroca R., Azcón R., Ruiz-Lozano J.M., PIP aquaporin gene expression in arbuscular mycorrhizal Glycine max and Lactuca sativa plants in relation to drought stress tolerance, Plant Mol. Biol., 2006, 60, 389–404 in Google Scholar PubMed

[33] Martre P., Morillon R., Barrieu F., North G.B., Nobel P.S., Chrispeels M.J., Plasma membrane aquaporins play a significant role during recovery from water deficit, Plant Physiol., 2002, 130, 2101–2110 in Google Scholar PubMed PubMed Central

[34] Sade N., Gebretsadik M., Seligmann R., Schwartz A., Wallach R., Moshelion M., The role of tobacco Aquaporin1 in improving water use efficiency, hydraulic conductivity, and yield production under salt stress, Plant Physiol., 2010, 152, 245–54 in Google Scholar PubMed PubMed Central

[35] Gao Y.P., Young L., Bonham-Smith P., Gusta L.V., Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions, Plant Mol. Biol., 1999, 40, 635–644 in Google Scholar

[36] Morillon R., Catterou M., Sangwan R.S., Sangwan B.S., Lassalles J.P., Brassinolide may control aquaporin activities in Arabidopsis thaliana, Planta, 2001, 212, 199–204 in Google Scholar PubMed

[37] Gu F., Hata R., Toku K., Yang L., Ma Y.J., Maeda, N., et al. Testosterone up-regulates aquaporin-4 expression in cultured astrocytes, J. Neurosci. Res., 2003, 72, 709–715 in Google Scholar PubMed

[38] Flexas J., Medrano H., Drought inhibition of photosynthesis in C3 Plants: stomatal and non stomatal limitations revisited, Ann. Bot., 2002, 89, 183–189 in Google Scholar PubMed PubMed Central

[39] Grunwald C., Effect of sterols on the permeability of alcohol-treated red beet tissue, Plant Physiol., 1968, 43, 484–488 in Google Scholar PubMed PubMed Central

[40] Grunwald C., Sterol molecular modifications influencing membrane permeability, Plant Physiol., 1974, 54, 624–628 in Google Scholar PubMed PubMed Central

[41] Saltveit M.E., Jr., Effect of alcohols and their interaction with ethylene on the ripening of epidermal pericarp discs of tomato fruit, Plant Physiol., 1989, 90, 167–174 in Google Scholar PubMed PubMed Central

[42] Yu J.Q., Huang L.F., Hu W.H., Zhou Y.H., Mao W.H., Ye S.F., et al., A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus, J. Exp. Bot., 2004, 55, 1135–1143 in Google Scholar PubMed

[43] Janeczko A., Swaczynová J., Endogenous brassinosteroids in wheat treated with 24-epibrassinolide, Biol. Plant., 2010, 54, 477–482 in Google Scholar

[44] Janeczko A., Biesaga-Kościelniak J., Oklestkova J., Filek M., Dziurka M., et al., Role of 24-epibrassinolide in wheat production: physiological effects and uptake, J. Agron. Crop Sci. 2010, 196, 311–321 10.1111/j.1439-037X.2009.00413.xSearch in Google Scholar

[45] Kelly M.O., Saltveit M.E., Jr., Effect of endogenously synthesized and exogenously applied ethanol on tomato fruit ripening, Plant Physiol., 1988, 88, 143–147 in Google Scholar PubMed PubMed Central

[46] Cossins E.A., Turner E.R., The metabolism of ethanol in germinating pea seedlings, J. Exp. Bot., 1963, 14, 290–298 in Google Scholar

[47] Cossins E.A., Beevers H., Ethanol metabolism in plant tissues, Plant Physiol., 1963, 38, 375–380 in Google Scholar PubMed PubMed Central

Published Online: 2012-8-3
Published in Print: 2012-10-1

© 2012 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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