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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access August 20, 2010

Molecular characterization of rotaviruses in mid-western Turkey, 2006–2007

  • Mustafa Altindis EMAIL logo , Krisztián Bányai , Raike Kalayci , Cihangir Gulamber , Resit Koken , Teoman Apan , Esra Koçoğlu , Aysegul Bukulmez , Yasin Yoldas and Pınar Aykurt
From the journal Open Medicine

Abstract

Vaccines against rotaviruses are now available in numerous countries, including Turkey. As the vaccines may show various efficiencies against different type specificities and routine vaccination in infants might result in selection and immune escape of wild-type rotavirus strains, strain surveillance has been initiated before and during the vaccine introduction. We aimed to provide corresponding information on local strain prevalence in Anatolia, mid-western Turkey during the introduction of rotavirus vaccines. Stool samples positive for group A rotavirus by commercial enzyme immunoassay were subjected to reverse transcription-polymerase chain reaction based genotyping of the outer capsid antigens, VP7 and VP4, determining G and P type specificities respectively. Among 36 fully and 5 partially typeable strains we detected genotype G1, G2, and G9 VP7 specificities and genotype P[4], P[6] and P[8] VP4 specificities in 5 individual and 4 mixed combinations. The most common strain was G2P[4] (n=17), followed by G9P[8] (n=9). Other strains were G1P[8] (n=2), G2P[8] (n=2), G1+2P[8] (n=2), G9P[4] (n=1), G2+9P[8] (n=1), G4+9P[6] (n=1), and G2P[4+8] (n=1). Partially typed strains included 2 G1P[NT] and 3 G2P[NT] strains. Our data may help determine a baseline of the rotavirus genotype prevalence in Turkey and see if changes in the incidence of individual strains will be observed after routine use of vaccine.

[1] Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI. Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 2003; 9: 565–572 10.3201/eid0905.020562Search in Google Scholar

[2] Dennehy PH. Rotavirus vaccines: an overview. Clin Microbiol Rev 2008; 21: 198–208 http://dx.doi.org/10.1128/CMR.00029-0710.1128/CMR.00029-07Search in Google Scholar

[3] Estes MK, Kapikian AZ. Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, et al, editors. Fields virology. 5th ed, Vol 2. Philadelphia: Lippincott Williams & Wilkins/Wolters Kluwer; 2006. p. 1917–1974 Search in Google Scholar

[4] Gentsch JR, Laird AR, Bielfelt B, et al. Serotype diversity and reassortment between human and animal rotavirus strains: implications for rotavirus vaccine programs. J Infect Dis 2005; 192Suppl 1: S146–159 http://dx.doi.org/10.1086/43149910.1086/431499Search in Google Scholar

[5] Santos N, Hoshino Y. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 2005; 15: 29–56 http://dx.doi.org/10.1002/rmv.44810.1002/rmv.448Search in Google Scholar

[6] Vesikari T, Matson DO, Dennehy P, et al. Safety and efficacy of a pentavalent human-bovine (WC3) reassortant rotavirus vaccine. N Engl J Med 2006; 354: 23–33 http://dx.doi.org/10.1056/NEJMoa05266410.1056/NEJMoa052664Search in Google Scholar

[7] Ruiz-Palacios GM, Pérez-Schael I, Velázquez FR, et al. Safety and efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N Engl J Med 2006; 354: 11–22 http://dx.doi.org/10.1056/NEJMoa05243410.1056/NEJMoa052434Search in Google Scholar

[8] Linhares AC, Velázquez FR, Pérez-Schael I, et al. Efficacy and safety of an oral live attenuated human rotavirus vaccine against rotavirus gastroenteritis during the first 2 years of life in Latin American infants: a randomised, double-blind, placebo-controlled phase III study. Lancet 2008; 371: 1181–1189 http://dx.doi.org/10.1016/S0140-6736(08)60524-310.1016/S0140-6736(08)60524-3Search in Google Scholar

[9] Jakab F, Meleg E, Bányai K, et al. One-year survey of astrovirus infection in children with gastroenteritis in a large hospital in Hungary: occurrence and genetic analysis of astroviruses. J Med Virol 2004; 74: 71–77 http://dx.doi.org/10.1002/jmv.2014810.1002/jmv.20148Search in Google Scholar PubMed

[10] Das BK, Gentsch JR, Cicirello HG, et al. Characterization of rotavirus strains from newborns in New Delhi, India. J Clin Microbiol 1994; 32: 1820–1822 10.1128/jcm.32.7.1820-1822.1994Search in Google Scholar PubMed PubMed Central

[11] Gentsch JR, Glass RI, Woods P, et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 1992; 30: 1365–1373 10.1128/jcm.30.6.1365-1373.1992Search in Google Scholar PubMed PubMed Central

[12] Bányai K, Gentsch JR, Schipp R, et al. Dominating prevalence of P[8],G1 and P[8],G9 rotavirus strains among children admitted to hospital between 2000 and 2003 in Budapest, Hungary. J Med Virol 2005; 76: 414–423 http://dx.doi.org/10.1002/jmv.2037210.1002/jmv.20372Search in Google Scholar PubMed

[13] Gouvea V, Glass RI, Woods P, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 1990; 28: 276–282 10.1128/jcm.28.2.276-282.1990Search in Google Scholar PubMed PubMed Central

[14] Kang G, Iturriza-Gomara M, Wheeler JG, et al. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: correlation with clinical severity in children in South India. J Med Virol 2004; 73: 118–122 http://dx.doi.org/10.1002/jmv.2005310.1002/jmv.20053Search in Google Scholar PubMed PubMed Central

[15] Ceyhan M, Kanra G, Yeniay I, Ciliv G, Vesikari T. Rotaviruses in infants with diarrhea studied by viral RNA electrophoresis in Ankara, Turkey. Turk J Pediatr 1987; 29: 145–149 Search in Google Scholar

[16] Kurugöl Z, Geylani S, Karaca Y, et al. Rotavirus gastroenteritis among children under five years of age in Izmir, Turkey. Turk J Pediatr 2003; 45: 290–294 Search in Google Scholar

[17] Altindis M, Yavru S, Simsek A, Ozkul A, Ceri A, Koc H. Rotavirus infection in children with acute diarrhea as detected by latex agglutination, ELISA and polyacrylamide gel electrophoresis. Indian Pediatr 2004; 41: 590–594 Search in Google Scholar

[18] Çataloluk O, Iturriza M, Gray J. Molecular characterization of rotaviruses circulating in the population in Turkey. Epidemiol Infect 2005; 133: 673–678 http://dx.doi.org/10.1017/S095026880500388210.1017/S0950268805003882Search in Google Scholar

[19] Karadag A, Acikgoz ZC, Avci Z, et al. Childhood diarrhoea in Ankara, Turkey: epidemiological and clinical features of rotavirus-positive versus rotavirus-negative cases. Scand J Infect Dis 2005; 37: 269–275 http://dx.doi.org/10.1080/0036554041002098310.1080/00365540410020983Search in Google Scholar PubMed

[20] Bozdayi G, Dogan B, Dalgic B, et al. Diversity of human rotavirus G9 among children in Turkey. J Med Virol 2008; 80: 733–740 http://dx.doi.org/10.1002/jmv.2112010.1002/jmv.21120Search in Google Scholar PubMed

[21] Altindis M, Bestepe G, Ceri A, Yavru S, Kalayci R. Frequency of rotavirus and enteric adenovirus infection in children with acute gastroenteritis. Med J SDU 2008; 15: 60–63 Search in Google Scholar

[22] Staat MA, Azimi PH, Berke T, et al. Clinical presentations of rotavirus infection among hospitalized children. Pediatr Infect Dis J 2002; 21: 221–227 http://dx.doi.org/10.1097/00006454-200203000-0001210.1097/00006454-200203000-00012Search in Google Scholar PubMed

[23] Maltezou HC, Zafiropoulou A, Mavrikou M, et al. Acute diarrhoea in children treated in an outpatient setting in Athens, Greece. J Infect 2001; 43: 122–127 http://dx.doi.org/10.1053/jinf.2001.084410.1053/jinf.2001.0844Search in Google Scholar PubMed

[24] Cardoso DD, Soares CM, Dias e Souza MB, de Azevedo Mda S, Martins RM, Queiróz DA. Epidemiological features of rotavirus infection in Goiânia, Goiás, Brazil, from 1986 to 2000. Mem Inst Oswaldo Cruz 2003; 98: 25–29 http://dx.doi.org/10.1590/S0074-0276200300010000410.1590/S0074-02762003000100004Search in Google Scholar PubMed

[25] Denno DM, Stapp JR, Boster DR, et al. Etiology of diarrhea in pediatric outpatient settings. Pediatr Infect Dis J 2005; 24: 142–148 http://dx.doi.org/10.1097/01.inf.0000151031.47761.6d10.1097/01.inf.0000151031.47761.6dSearch in Google Scholar PubMed

[26] O’Ryan M, Díaz J, Mamani N, Navarrete M, Vallebuono C. Impact of rotavirus infections on outpatient clinic visits in Chile. Pediatr Infect Dis J 2007; 26: 41–45 http://dx.doi.org/10.1097/01.inf.0000247104.01291.7110.1097/01.inf.0000247104.01291.71Search in Google Scholar PubMed

[27] Yokoo M, Arisawa K, Nakagomi O. Estimation of annual incidence, age-specific incidence rate, and cumulative risk of rotavirus gastroenteritis among children in Japan. Jpn J Infect Dis 2004; 57: 166–171 Search in Google Scholar

[28] Bányai K, Sas Y, Varga L, Szucs G. Survey of rotavirus infection in a Hungarian paediatric hospital. Acta Microbiol Immunol Hung 2004; 51: 431–435 http://dx.doi.org/10.1556/AMicr.51.2004.4.310.1556/AMicr.51.2004.4.3Search in Google Scholar PubMed

[29] Meeting of the Immunization Strategic Advisory Group of Experts, April 2009—conclusions and recommendations. Wkly Epidemiol Rec 2009; 84: 220–236 Search in Google Scholar

Published Online: 2010-8-20
Published in Print: 2010-10-1

© 2010 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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