Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access June 12, 2013

Role of antioxidant vitamins administration on the oxidative stress

  • Miki Tomoeda EMAIL logo , Chiaki Kubo , Hidenori Yoshizawa , Michiko Yuki , Masanori Kitamura , Shigenori Nagata , Masahito Murakami , Yasuko Nishizawa and Yasuhiko Tomita
From the journal Open Medicine


The health-promoting effects of antioxidant vitamins C and E supplementation are unclear. This study investigated the effects of vitamins C and E on the activities of reactive oxygen species (ROS)-scavenging enzymes and protein and lipid peroxidation statuses under resting and exercise-induced conditions. Thirteen healthy, previously untrained males (age 20–21 years) participated in this study. Seven subjects performed physical exercise using a cycle ergometer, and six performed a 6-min walk test (6MWT) prior to vitamin administration and after 1-week oral administration of vitamin C (1000 mg/day) and vitamin E (300 IU/day). Venous blood samples were collected before and after exercise. Plasma vitamin C concentration, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and protein carbonyl and thiobarbituric acid-reactive substance (TBARS) contents were measured. Antioxidant supplementation increased vitamin C concentration by 34% (p<0.05), decreased SOD activity by 17% (p<0.05), increased GPx activity by 13% (p<0.05), and increased the GPx/SOD activity ratio by 37% (p<0.05). Protein carbonyl and TBARS contents were unaffected. Antioxidant vitamins effectively increase the plasma GPx/SOD activity ratio, but fail to reduce protein carbonyl levels induced by exercise.

[1] Halliwell B., Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans, Free Radic. Res. 1996, 25, 57–74 in Google Scholar PubMed

[2] Davies K.J., Quintanilha A.T., Brooks G.A., Packer L., Free radicals and tissue damage produced by exercise, Biochem. Biophys. Res. Commun. 1982, 107, 1198–1205 in Google Scholar PubMed

[3] Commoner B., Townsend J., Pake G.E., Free radicals in biological materials, Nature 1954, 174, 689–691 in Google Scholar PubMed

[4] Sastre J., Asensi M., Gasco E., Pallardo F.V., Ferrero J.A., Furukawa T., et al., Exhaustive physical exercise causes oxidation of glutathione status in blood: prevention by antioxidant administration, Am. J. Physiol. 1992, 263, R992–995 10.1152/ajpregu.1992.263.5.R992Search in Google Scholar PubMed

[5] Goldfarb A.H., McKenzie M.J., Bloomer R.J., Gender comparisons of exercise-induced oxidative stress: influence of antioxidant supplementation, Appl. Physiol. Nutr. Metab. 2007, 32, 1124–1131 in Google Scholar PubMed

[6] Ryan M.J., Dudash H.J., Docherty M., Geronilla K.B., Baker B.A., Haff G.G., et al., Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes and positive muscle work in chronically loaded muscles of aged rats, Exp. Gerontol. 2010, 45, 882–895 in Google Scholar PubMed PubMed Central

[7] Xu X.M., Moller S.G., ROS removal by DJ-1: Arabidopsis as a new model to understand Parkinson’s Disease, Plant. Signal. Behav. 2010, 5, 1034–1036 in Google Scholar PubMed PubMed Central

[8] Elchuri S., Oberley T.D., Qi W., Eisenstein R.S., Jackson Roberts L., Van Remmen H., et al., CuZnSOD deficiency leads to persistent and widespread oxidative damage and hepatocarcinogenesis later in life, Oncogene 2005, 24, 367–380 in Google Scholar PubMed

[9] Muller F.L., Song W., Liu Y., Chaudhuri A., Pieke-Dahl S., Strong R., et al., Absence of CuZn superoxide dismutase leads to elevated oxidative stress and acceleration of age-dependent skeletal muscle atrophy, Free Radic. Biol. Med. 2006, 40, 1993–2004 in Google Scholar PubMed

[10] Gomez-Cabrera M.C., Domenech E., Romagnoli M., Arduini A., Borras C., Pallardo F.V., et al., Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers traininginduced adaptations in endurance performance, Am. J. Clin. Nutr. 2008, 87, 142–149 10.1093/ajcn/87.1.142Search in Google Scholar PubMed

[11] Powers S.K., Duarte J., Kavazis A.N., Talbert E.E., Reactive oxygen species are signalling molecules for skeletal muscle adaptation, Exp. Physiol. 2010, 95, 1–9 in Google Scholar PubMed PubMed Central

[12] Higashida K., Kim S.H., Higuchi M., Holloszy J.O., Han D.H., Normal adaptations to exercise despite protection against oxidative stress, Am. J. Physiol. Endocrinol. Metab. 2011, 301, E779–784 in Google Scholar PubMed PubMed Central

[13] Theodorou A.A., Nikolaidis M.G., Paschalis V., Koutsias S., Panayiotou G., Fatouros I.G., et al., No effect of antioxidant supplementation on muscle performance and blood redox status adaptations to eccentric training, Am. J. Clin. Nutr. 2011, 93, 1373–1383 in Google Scholar PubMed

[14] Yfanti C., Akerstrom T., Nielsen S., Nielsen A.R., Mounier R., Mortensen O.H., et al., Antioxidant supplementation does not alter endurance training adaptation, Med. Sci. Sports. Exerc. 2010, 42, 1388–1395 10.1249/MSS.0b013e3181cd76beSearch in Google Scholar PubMed

[15] Ristow M., Zarse K., Oberbach A., Kloting N., Birringer M., Kiehntopf M., et al., Antioxidants prevent health-promoting effects of physical exercise in humans, Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 8665–8670 in Google Scholar PubMed PubMed Central

[16] Nikolaidis M.G., Kerksick C.M., Lamprecht M., McAnulty S.R., Does vitamin C and E supplementation impair the favorable adaptations of regular exercise?, Oxid. Med. Cell. Longev. 2012, 2012, 707941 10.1155/2012/707941Search in Google Scholar PubMed PubMed Central

[17] Halliwell B., Biochemistry of oxidative stress, Biochem. Soc. Trans. 2007, 35, 1147–1150 in Google Scholar PubMed

[18] Retana-Ugalde R., Casanueva E., Altamirano-Lozano M., Gonzalez-Torres C., Mendoza-Nunez V.M., High dosage of ascorbic acid and alphatocopherol is not useful for diminishing oxidative stress and DNA damage in healthy elderly adults, Ann. Nutr. Metab. 2008, 52, 167–173 in Google Scholar PubMed

[19] Shargorodsky M., Debby O., Matas Z., Zimlichman R., Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors, Nutr. Metab. (Lond) 2010, 7, 55 in Google Scholar PubMed PubMed Central

[20] Salonen J.T., Nyyssonen K., Salonen R., Lakka H.M., Kaikkonen J., Porkkala-Sarataho E., et al., Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) study: a randomized trial of the effect of vitamins E and C on 3-year progression of carotid atherosclerosis, J. Intern. Med. 2000, 248, 377–386 in Google Scholar PubMed

[21] Lapointe A., Couillard C., Lemieux S., Effects of dietary factors on oxidation of low-density lipoprotein particles, J. Nutr. Biochem. 2006, 17, 645–658 in Google Scholar PubMed

[22] Hercberg S., Kesse-Guyot E., Druesne-Pecollo N., Touvier M., Favier A., Latino-Martel P., et al., Incidence of cancers, ischemic cardiovascular diseases and mortality during 5-year follow-up after stopping antioxidant vitamins and minerals supplements: a postintervention follow-up in the SU.VI. MAX Study, Int. J. Cancer 2010, 127, 1875–1881 in Google Scholar PubMed

[23] Omar B.A., McCord J.M., The cardioprotective effect of Mn-superoxide dismutase is lost at high doses in the postischemic isolated rabbit heart, Free Radic. Biol. Med. 1990, 9, 473–478 in Google Scholar PubMed

[24] Rosen D.R., Siddique T., Patterson D., Figlewicz D.A., Sapp P., Hentati A., et al., Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis, Nature 1993, 362, 59–62 in Google Scholar PubMed

[25] Amstad P., Peskin A., Shah G., Mirault M.E., Moret R., Zbinden I., et al., The balance between Cu,Znsuperoxide dismutase and catalase affects the sensitivity of mouse epidermal cells to oxidative stress, Biochemistry 1991, 30, 9305–9313 in Google Scholar PubMed

[26] Amstad P., Moret R., Cerutti P., Glutathione peroxidase compensates for the hypersensitivity of Cu,Zn-superoxide dismutase overproducers to oxidant stress, J. Biol. Chem. 1994, 269, 1606–1609 10.1016/S0021-9258(17)42068-0Search in Google Scholar

[27] Park E.M., Ramnath N., Yang G.Y., Ahn J.Y., Park Y., Lee T.Y., et al., High superoxide dismutase and low glutathione peroxidase activities in red blood cells predict susceptibility of lung cancer patients to radiation pneumonitis, Free Radic. Biol. Med. 2007, 42, 280–287 in Google Scholar PubMed PubMed Central

[28] Nakhostin-Roohi B., Barmaki S., Khoshkhahesh F., Bohlooli S., Effect of chronic supplementation with methylsulfonylmethane on oxidative stress following acute exercise in untrained healthy men, J. Pharm. Pharmacol. 2011, 63, 1290–1294 in Google Scholar PubMed

Published Online: 2013-6-12
Published in Print: 2013-8-1

© 2013 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 24.2.2024 from
Scroll to top button