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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 1, 2011

First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia

  • Katarína Reiterová EMAIL logo , Silvia Špilovská , Andrea Čobádiová and Rastislav Mucha
From the journal Acta Parasitologica

Abstract

The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion, cultured on Vero cells. Neospora-like tachyzoites were first observed after 77 days of cultivation. The parasite was confirmed by polymerase chain reaction (PCR) using Neospora-specific primers Np21 and Np6. The PCR product of the first Slovak isolate (NC-SKB1) was subsequently sequenced and published in GenBank under accession number GU300774. Sequencing and BLAST search identified the isolate as N. caninum.

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Published Online: 2011-6-1
Published in Print: 2011-6-1

© 2011 W. Stefański Institute of Parasitology, PAS

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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