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Improved DPPH determination for antioxidant activity spectrophotometric assay

  • M. Szabo EMAIL logo , C. Idiţoiu , D. Chambre and A. Lupea
From the journal Chemical Papers


An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.

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Published Online: 2007-6-1
Published in Print: 2007-6-1

© 2007 Institute of Chemistry, Slovak Academy of Sciences

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