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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 13, 2013

Protective effect of tea polyphenols on the blood-brain barrier

  • Rongliang Xue EMAIL logo , Jianrui Lv , Jing Gao , Rongguo Fu , Wei Li , Xiaoming Lei , Gang Wu , Li Xue and Zhenni Zhang


This study was to investigate the protective effects of tea polyphenols on the blood-brain barrier (BBB) of rats with global cerebral ischemia/reperfusion (GCIR) injury. Sprague Dawley rats underwent four-vessel occlusion to construct the model of GCIR. Half an hour before complete occlusion, they were treated with tea polyphenols (TP) (6.4%; 100 or 200 mg/kg) via tail intravenous injection. 24 h after reperfusion, BBB permeability was evaluated by measuring brain water content (BWC) and residual amount of Evan’s blue dye in cerebral tissue. In addition to this, MMP-9 and collagen IV protein expression in cerebral tissue were also detected using immunohistochemistry. ANOVA and SNK-q were used to do statistical analysis. Statistical significance was considered at P<0.05. Compared to the untreated, the TP-treated rats had significantly decreased BWC (P<0.05), decreased residual amount of Evan’s blue dye in cerebral tissue (P<0.05), down-regulated MMP-9 (P<0.05) and up-regulated collagen IV expression in brain tissue (P<0.05). It can be concluded from these findings that TP may reduce the MMP-9 mediated collagen IV degradation caused by GCIR to protect the BBB.

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Published Online: 2013-9-13
Published in Print: 2013-9-1

© 2013 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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