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BY-NC-ND 3.0 license Open Access Published by De Gruyter February 26, 2014

PCR detection of Neospora caninum in water buffalo foetal tissues

  • Clementina Auriemma EMAIL logo , Maria Lucibelli , Giorgia Borriello , Esterina Carlo , Alessandra Martucciello , Lorena Schiavo , Amalia Gallo , Francesca Bove , Federica Corrado , Santa Girardi , Maria Amoroso , Barbara Ďegli Uberti and Giorgio Galiero
From the journal Acta Parasitologica

Abstract

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

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Published Online: 2014-2-26
Published in Print: 2014-3-1

© 2014 W. Stefański Institute of Parasitology, PAS

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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