The adsorption and penetration of poliovirus-RNA in tissue culture cells are two processes which can be separated experimentally. The process of adsorption can be interrupted by diluting the RNA at certain times after seeding whereas the process of penetration can be followed by the application of RNase. The experimental results reported lead to the following conclusions:
1. The cells are sensitized for uptake of RNA by hypertonic saline.
2. As long as the cells are in hypertonic saline the majority of RNA remains in a state susceptible to R Nase.
3. At the time the cell environment is changed from hypertonicity to isotonicity the adsorbed RNA enters the cells and is no longer sensitive to the action of external RNase even when the RNase is now applied in hypertonic solution.
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