Mango stem bark extracts (MSBE) have been used as bioactive ingredients for nutraceutical, cosmeceutical, and pharmaceutical formulations due to their antioxidant, anti-inflammatory, and analgesic effects. We performed the MSBE preparative column liquid chromatography, which led to the resolution and identification by GC-MS of 64 volatile compounds: 7 hydrocarbons, 3 alcohols, 1 ether, 3 aldehydes/ketones, 7 phenols, 20 terpenoids (hydrocarbons and oxygenated derivatives), 9 steroids, 4 nitrogen compounds, and 1 sulphur compound. Major components were β -elemene, α -guaiene, aromadendrene, hinesol, 1-octadecene, β -eudesmol, methyl linoleate, juniper camphor, hinesol, 9-methyl (3 β ,5 α )-androstan-3-ol, γ-sitosterol, β -chamigrene, 2,5-dihydroxymethyl-phenetylalcohol, N -phenyl-2-naphtaleneamine, and several phenolic compounds. The analysis of MSBE, Haden variety, by GC-MS is reported for the first time, which gives an approach to understand the possible synergistic effect of volatile compounds on its antioxidant, analgesic, and anti-inflammatory effects. The identification of relevant bioactive volatile components from MSBE extracts, mainly terpenes from the eudesmane family, will contribute to correlate its chemical composition to previous determined pharmacological effects.