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October 15, 2017
Abstract
Three recombinant antigens viz . arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T . canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T . canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T . canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii , Plasmodium vivax , Entamoeba histolytica , hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T . gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T . gondii , P . vivax and E . histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T . canis larvae.