Open Access Published since August 25, 2010

Biomolecular Concepts

ISSN: 1868-503X

Editor-in-chief: Daniela Giacomazza

About this journal

Objective
BioMolecular Concepts is a peer-reviewed open access journal fostering the integration of different fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers, and conclusive extensions of research data leading to new and original, testable hypotheses. Aspects of research that can promote related fields, and lead to novel insight into biological mechanisms or potential medical applications are of special interest. Original research articles reporting new data of broad significance are also welcome.

Topics

cellular and molecular biology
genetics and epigenetics
biochemistry
structural biology
neurosciences
developmental biology
molecular medicine
pharmacology
microbiology
plant biology and biotechnology.

Article formats
review articles, short conceptual overviews, original research articles

> Information on submission process

Journal Partner:

SIBPA

Your Benefits

Your benefits:

Interdisciplinary research
Unrestricted access for all readers
Fast, comprehensive and transparent peer-review
Free language assistance
Comprehensive abstracting & indexing - PubMed, BIOSIS Previews (Web of Science), SCOPUS
Promotion and worldwide distribution of each published article

History

Founded in 2010, BioMolecular Concepts intends to fill a gap in the current literature in biological research by providing a conceptual platform favoring the development of novel ideas and cross-discipline scientific views, with a focus on research resulting in paradigm changes. Reviews cover novel and original concepts arising from recent findings in forefront fields of biology, summaries of research within these fields, and conclusive extensions resulting in new, testable hypotheses. Of special interest are aspects that can promote related biomolecular and biomedical fields, and how they can lead to more biological insight or potential medical applications.

Other publications in the field
• Biological Chemistry
• Open Life Sciences
• Advances in Cell Biology
• Journal of Medical Biochemistry

Abstracting and Indexing

Biomolecular Concepts is covered by the following services:

Baidu Scholar
Cabells Journalytics
Chemical Abstracts Service (CAS) - CAplus
Chemical Abstracts Service (CAS) - SciFinder
Chronos Hub
CNKI Scholar (China National Knowledge Infrastructure)
CNPIEC - cnpLINKer
Dimensions
DOAJ (Directory of Open Access Journals)
EBSCO (relevant databases)
EBSCO Discovery Service
EMBASE
Genamics JournalSeek
GoOA
Google Scholar
Index Copernicus
J-Gate
JournalGuide
JournalTOCs
KESLI-NDSL (Korean National Discovery for Science Leaders)
Medline
MyScienceWork
Naver Academic
Naviga (Softweco)
Primo Central (ExLibris)
ProQuest (relevant databases)
Publons
PubMed
QOAM (Quality Open Access Market)
ReadCube
Reaxys
SCImago (SJR)
SCOPUS
Semantic Scholar
Sherpa/RoMEO
Summon (ProQuest)
TDNet
Ulrich's Periodicals Directory/ulrichsweb
WanFang Data
Web of Science - BIOSIS Previews
Web of Science - BIOSIS Reviews Reports and Meetings
WorldCat (OCLC)
X-MOL
Yewno Discover

Supplementary Materials

Topics

External Links

Volume 13 Issue 1

January 2022

Research Articles

Open Access February 22, 2022

Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.)

Roksana Bobrowska, Aleksandra Noweiska, Julia Spychała, Agnieszka Tomkowiak, Jerzy Nawracała, Michał T. Kwiatek

Page range: 1-9

More Download PDF

Abstract

Wheat leaf rust, caused by fungal pathogen Puccinia triticina Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called “slow rusting” genes using marker-assisted selection (MAS). Until now, eight genes ( Lr34/Yr18 , Lr46/Yr29 , Lr67/Yr46 , Lr68 , Lr74 , Lr75 , Lr77 , and Lr78 ) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as “false positives.” The aim of this study was to compare the available four markers: Xwmc44 , Xgwm259 , Xbarc80 , and csLV46G22 markers (not published yet), for the identification of the Lr46/Yr29 loci in 73 genotypes of wheat, which were reported as sources of various “slow rusting” genes, including 60 with confirmed Lr46/Yr29 gene, reported in the literature. This research revealed that csLV46G22 together with Xwmc44 is most suitable for the identification of resistance allele of the Lr46/Yr29 gene; however, there is a need to clone the Lr46/Yr29 loci to identify and verify the allelic variation of the gene and the function.

Open Access February 21, 2022

NADPH-derived ROS generation drives fibrosis and endothelial-to-mesenchymal transition in systemic sclerosis: Potential cross talk with circulating miRNAs

Anna Maria Posadino, Gian Luca Erre, Annalisa Cossu, Costanza Emanueli, Ali H. Eid, Angelo Zinellu, Gianfranco Pintus, Roberta Giordo

Page range: 11-24

More Download PDF

Abstract

Systemic sclerosis (SSc) is an immune disorder characterized by diffuse fibrosis and vascular abnormalities of the affected organs. Although the etiopathology of this disease is largely unknown, endothelial damage and oxidative stress appear implicated in its initiation and maintenance. Here, we show for the first time that circulating factors present in SSc sera increased reactive oxygen species (ROS) production, collagen synthesis, and proliferation of human pulmonary microvascular endothelial cells (HPMECs). The observed phenomena were also associated with endothelial to mesenchymal transition (EndMT) as indicated by decreased von Willebrand factor (vWF) expression and increased alpha-smooth muscle actin, respectively, an endothelial and mesenchymal marker. SSc-induced fibroproliferative effects were prevented by HPMECs exposition to the NADPH oxidase inhibitor diphenyleneiodonium, demonstrating ROS’s causative role and suggesting their cellular origin. Sera from SSc patients showed significant changes in the expression of a set of fibrosis/EndMT-associated microRNAs (miRNA), including miR-21, miR-92a, miR-24, miR-27b, miR-125b, miR-29c, and miR-181b, which resulted significantly upregulated as compared to healthy donors sera. However, miR29b resulted downregulated in SSc sera, whereas no significant differences were found in the expression of miR-29a in the two experimental groups of samples. Taking together our data indicate NADPH oxidase-induced EndMT as a potential mechanism of SSc-associated fibrosis, suggesting fibrosis-associated miRNAs as potentially responsible for initiating and sustaining the vascular alterations observed in this pathological condition.

Open Access February 21, 2022

Effect of omega-3 fatty acids on the telomere length: A mini meta-analysis of clinical trials

Sawan Ali, Giovanni Scapagnini, Sergio Davinelli

Page range: 25-33

More Download PDF

Abstract

Telomeres are protective caps at the end of eukaryotic chromosomes, whose length is correlated with health and lifespan. Telomere attrition is a common feature of the aging process and can be accelerated by oxidative stress and chronic inflammation. Various nutrients influence the telomere length, partially due to their antioxidant and anti-inflammatory properties. The aim of this review was to meta-analytically assess the effect of omega-3 fatty acids on the telomere length. We searched four databases (PubMed, Web of Sciences, Scopus, and the Cochrane Library) from inception until November 2021. Of 573 records, a total of 5 clinical trials were included for the quantitative meta-analysis, comprising a total of 337 participants. The results revealed an overall beneficial effect of omega-3 fatty acids on the telomere length (mean difference = 0.16; 95% CI, 0.02, 0.30; p = 0.02). Despite a limited number of studies, the available evidence suggests that omega-3 fatty acids may positively affect the telomere length. However, larger clinical trials are needed to confirm our findings, along with studies aimed to clarify the underlying molecular mechanisms.

Open Access February 21, 2022

Analysis of differentially expressed genes and signaling pathways involved in atherosclerosis and chronic obstructive pulmonary disease

Stanislav Kotlyarov

Page range: 34-54

More Download PDF

Abstract

Atherosclerosis is an important medical and social problem, and the keys to solving this problem are still largely unknown. A common situation in real clinical practice is the comorbid course of atherosclerosis with chronic obstructive pulmonary disease (COPD). Diseases share some common risk factors and may be closely linked pathogenetically. Methods: Bioinformatics analysis of datasets from Gene Expression Omnibus (GEO) was performed to examine the gene ontology (GO) of common differentially expressed genes (DEGs) in COPD and peripheral arterial atherosclerosis. DEGs were identified using the limma R package with the settings p < 0.05, corrected using the Benjamini & Hochberg algorithm and ǀlog 2FCǀ > 1.0. The GO, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and the protein–protein interaction (PPI) network analysis were performed with the detected DEGs. Results: The biological processes and signaling pathways involving common DEGs from airway epithelial datasets in COPD and tissue in peripheral atherosclerosis were identified. A total of 15 DEGs were identified, comprising 12 upregulated and 3 downregulated DEGs. The GO enrichment analysis demonstrated that the upregulated hub genes were mainly involved in the inflammatory response, reactive oxygen species metabolic process, cell adhesion, lipid metabolic process, regulation of angiogenesis, icosanoid biosynthetic process, and cellular response to a chemical stimulus. The KEGG pathway enrichment analysis demonstrated that the common pathways were Toll-like receptor signaling pathway, NF-kappa B signaling pathway, lipid and atherosclerosis, and cytokine–cytokine receptor interaction. Conclusions: Biological processes and signaling pathways associated with the immune response may link the development and progression of COPD and atherosclerosis.

Open Access February 21, 2022

The epigenetic dimension of protein structure

Fodil Azzaz, Jacques Fantini

Page range: 55-60

More Download PDF

Abstract

Accurate prediction of protein structure is one of the most challenging goals of biology. The most recent achievement is AlphaFold, a machine learning method that has claimed to have solved the structure of almost all human proteins. This technological breakthrough has been compared to the sequencing of the human genome. However, this triumphal statement should be treated with caution, as we identified serious flaws in some AlphaFold models. Disordered regions are often represented by large loops that clash with the overall protein geometry, leading to unrealistic structures, especially for membrane proteins. In fact, AlphaFold comes up against the notion that protein folding is not solely determined by genomic information. We suggest that all parameters controlling the structure of a protein without being strictly encoded in its amino acid sequence should be coined “epigenetic dimension of protein structure.” Such parameters include for instance protein solvation by membrane lipids, or the structuration of disordered proteins upon ligand binding, but exclude sequence-encoded sites of post-translational modifications such as glycosylation. In our view, this paradigm is necessary to reconcile two opposite properties of living systems: beyond rigorous biological coding, evolution has given way to a certain level of uncertainty and anarchy.

Open Access March 5, 2022

Gamma-induced mutants of Bacillus and Streptomyces display enhanced antagonistic activities and suppression of the root rot and wilt diseases in pulses

Ariyan Manikandan, Iruthayasamy Johnson, Nanjundan Jaivel, Ramasamy Krishnamoorthy, Murugaiyan SenthilKumar, Rajasekaran Raghu, Nellaiappan Olaganathan Gopal, Prasun K. Mukherjee, Rangasamy Anandham

Page range: 103-118

More Download PDF

Abstract

This study aims to increase Bacillus and Streptomyces antagonistic activity against the root rot and wilt diseases of pulses caused by Macrophomina phaseolina and Fusarium oxysporum f. sp. udum , respectively. To increase antagonistic action, Bacillus subtilis BRBac4, Bacillus siamensis BRBac21, and Streptomyces cavourensis BRAcB10 were subjected to random mutagenesis using varying doses of gamma irradiation (0.5–3.0 kGy). Following the irradiation, 250 bacterial colonies were chosen at random for each antagonistic strain and their effects against pathogens were evaluated in a plate assay. The ERIC, BOX, and random amplified polymorphic studies demonstrated a clear distinction between mutant and wild-type strains. When mutants were compared to wild-type strains, they showed improved plant growth-promoting characteristics and hydrolytic enzyme activity. The disease suppression potential of the selected mutants, B . subtilis BRBac4-M6, B . siamensis i BRBac21-M10, and S . cavourensis BRAcB10-M2, was tested in green gram, black gram, and red gram. The combined inoculation of B . siamensis BRBac21-M10 and S . cavourensis BRAcB10-M2 reduced the incidence of root rot and wilt disease. The same treatment also increased the activity of the defensive enzymes peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. These findings suggested that gamma-induced mutation can be exploited effectively to improve the biocontrol characteristics of Bacillus and Streptomyces . Following the field testing, a combined bio-formulation of these two bacteria may be utilised to address wilt and root-rot pathogens in pulses.

Open Access April 19, 2022

Corticosterone potentiates ochratoxin A-induced microglial activation

Anchana Chansawhang, Sataporn Phochantachinda, Piya Temviriyanukul, Boonrat Chantong

Page range: 230-241

More Download PDF

Abstract

Microglial activation in the central nervous system (CNS) has been associated with brain damage and neurodegenerative disorders. Ochratoxin A (OTA) is a mycotoxin that occurs naturally in food and feed and has been associated with neurotoxicity, while corticosteroids are CNS’ physiological function modulators. This study examined how OTA affected microglia activation and how corticosteroids influenced microglial neuroinflammation. Murine microglial cells (BV-2) were stimulated by OTA, and the potentiation effects on OTA-induced inflammation were determined by corticosterone pre-treatment. Expressions of pro-inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) were determined. Phosphorylation of mitogen-activated protein kinases (MAPKs) was analyzed by western blotting. OTA significantly increased the mRNA expression of IL-6, TNF-α, IL-1β, and iNOS and also elevated IL-6 and NO levels. Corticosterone pre-treatment enhanced the neuroinflammatory response to OTA in a mineralocorticoid receptor (MR)-dependent mechanism, which is associated with increases in extracellular signal-regulated kinase (ERK) and p38 MAPK activation. In response to OTA, microglial cells produced pro-inflammatory cytokines and NO, while corticosterone increased OTA-induced ERK and p38 MAPK phosphorylation via MR. Findings indicated the direct role of OTA in microglia activation and neuroinflammatory response and suggested that low corticosterone concentrations in the brain exacerbated neurodegeneration.

Open Access May 26, 2022

Supercomplex supercomplexes: Raison d’etre and functional significance of supramolecular organization in oxidative phosphorylation

Sunil Nath

Page range: 272-288

More Download PDF

Abstract

Following structural determination by recent advances in electron cryomicroscopy, it is now well established that the respiratory Complexes I–IV in oxidative phosphorylation (OXPHOS) are organized into supercomplexes in the respirasome. Nonetheless, the reason for the existence of the OXPHOS supercomplexes and their functional role remains an enigma. Several hypotheses have been proposed for the existence of these supercomplex supercomplexes. A commonly-held view asserts that they enhance catalysis by substrate channeling. However, this – and other views – has been challenged based on structural and biophysical information. Hence, new ideas, concepts, and frameworks are needed. Here, a new model of energy transfer in OXPHOS is developed on the basis of biochemical data on the pure competitive inhibition of anionic substrates like succinate by the classical anionic uncouplers of OXPHOS (2,4-dinitrophenol, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, and dicoumarol), and pharmacological data on the unique site-selective, energy-linked inhibition of energy conservation pathways in mitochondria induced by the guanidine derivatives. It is further found that uncouplers themselves are site-specific and exhibit differential selectivity and efficacy in reversing the inhibition caused by the Site 1/Complex I or Site 2/Complexes II–III-selective guanidine derivatives. These results lead to new vistas and sufficient complexity in the network of energy conservation pathways in the mitochondrial respiratory chain that necessitate discrete points of interaction with two classes of guanidine derivatives and uncoupling agents and thereby separate and distinct energy transfer pathways between Site 1 and Site 2 and the intermediate that energizes adenosine triphosphate (ATP) synthesis by Complex V. Interpretation based on Mitchell’s single-ion chemiosmotic theory that postulates only a single energy pool is inadequate to rationalize the data and account for the required complexity. The above results and available information are shown to be explained by Nath’s two-ion theory of energy coupling and ATP synthesis, involving coupled movement of succinate anions and protons, along with the requirement postulated by the theory for maintenance of homeostasis and ion translocation across the energy-transducing membrane of both succinate monoanions and succinate dianions by Complexes I–V in the OXPHOS supercomplexes. The new model of energy transfer in mitochondria is mapped onto the solved structures of the supercomplexes and integrated into a consistent model with the three-dimensional electron microscope computer tomography visualization of the internal structure of the cristae membranes in mammalian mitochondria. The model also offers valuable insights into diseased states induced in type 2 diabetes and especially in Alzheimer’s and other neurodegenerative diseases that involve mitochondrial dysfunction.

Open Access September 1, 2022

Insights into functional connectivity in mammalian signal transduction pathways by pairwise comparison of protein interaction partners of critical signaling hubs

Chilakamarti V. Ramana

Page range: 298-313

More Download PDF

Abstract

Growth factors and cytokines activate signal transduction pathways and regulate gene expression in eukaryotes. Intracellular domains of activated receptors recruit several protein kinases as well as transcription factors that serve as platforms or hubs for the assembly of multi-protein complexes. The signaling hubs involved in a related biologic function often share common interaction proteins and target genes. This functional connectivity suggests that a pairwise comparison of protein interaction partners of signaling hubs and network analysis of common partners and their expression analysis might lead to the identification of critical nodes in cellular signaling. A pairwise comparison of signaling hubs across several related pathways might reveal novel signaling modules. Analysis of p rotein i nteraction c onnectome by V enn (PIC-Venn) of transcription factors STAT1, STAT3, NFKB1, RELA, FOS, and JUN, and their common interaction network suggested that BRCA1 and TSC22D3 function as critical nodes in immune responses by connecting the signaling hubs into signaling modules. Transcriptional regulation of critical hubs may play a major role in the lung epithelial cells in response to SARS-CoV-2 and in COVID-19 patients. Mutations and differential expression levels of these critical nodes and modules in pathological conditions might deregulate signaling pathways and their target genes involved in inflammation. Biological connectivity emerges from the structural connectivity of interaction networks across several signaling hubs in related pathways. The main objectives of this study are to identify critical hubs, critical nodes, and modules involved in the signal transduction pathways of innate and adaptive immunity. Application of PIC-Venn to several signaling hubs might reveal novel nodes and modules that can be targeted by small regulatory molecules to simultaneously activate or inhibit cell signaling in health and disease.

Open Access October 31, 2022

The effects of supplementation of Nannochloropsis oculata microalgae on biochemical, inflammatory and antioxidant responses in diabetic rats

Ali Fereidouni, Ali Khaleghian, Neda Mousavi-Niri, Nasrollah Moradikor

Page range: 314-321

More Download PDF

Abstract

Diabetes is accompanied by inflammation and oxidation. Supplementation of anti-inflammatory and antioxidant compounds can prevent the progression of diabetes. This study aimed to investigate the effects of supplementation of Nannochloropsis oculata microalgae (NOM) on the inflammatory and antioxidant responses in diabetic rats. Sixty male rats were divided into six groups as diabetic and non-diabetic rats receiving 0, 10 and 20 mg/kg of body weight of NOM daily for 21 days. Body weight, the serum concentrations of insulin and glucose and the tissue concentrations of interleukin-1β (IL-1β), tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB), interleukin-6 (IL-6), malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), superoxide dismutase (SOD), glutathione peroxidase (GPx) were assessed. The results showed that induction of diabetes significantly reduced the body weight, the serum concentrations of insulin and the tissue concentrations of SOD, FRAP and GPx while increasing the concentrations of glucose, MDA, IL-1β, IL-6, NF-κB and TNF-α. Daily oral administration of NOM (10 and 20 mg/kg) significantly maintained the body weight, the serum concentrations of insulin and the tissue concentrations of SOD, FRAP and GPx while preventing the increase in the concentrations of glucose, MDA, IL-1β and TNF-α. In conclusion, diabetes caused inflammation and oxidation while NOM worked as a natural anti-inflammatory and antioxidant compound.

Review Articles

Open Access March 2, 2022

Interaction of cervical microbiome with epigenome of epithelial cells: Significance of inflammation to primary healthcare

Veronika Holubekova, Zuzana Kolkova, Ivana Kasubova, Marek Samec, Alena Mazurakova, Lenka Koklesova, Peter Kubatka, Tomas Rokos, Erik Kozubik, Kamil Biringer, Erik Kudela

Page range: 61-80

More Download PDF

Abstract

One pillar of the predictive, preventive, and personalized medicine framework strategies is the female health. The evaluation of women’s lifestyle and dietary habits in context with genetic and modifiable risk factors may reflect the prevention of cervical cancer before the occurrence of clinical symptoms and prediction of cervical lesion behavior. The main aim of this review is to analyze publications in the field of precision medicine that allow the use of research knowledge of cervical microbiome, epigenetic modifications, and inflammation in potential application in clinical practice. Personalized approach in evaluating patient’s risk of future development of cervical abnormality should consider the biomarkers of the local microenvironment characterized by the microbial composition, epigenetic pattern of cervical epithelium, and presence of chronic inflammation. Novel sequencing techniques enable a more detailed characterization of actual state in cervical epithelium. Better understanding of all changes in multiomics level enables a better assessment of disease prognosis and selects the eligible targeted therapy in personalized medicine. Restoring of healthy vaginal microflora and reversing the outbreak of cervical abnormality can be also achieved by dietary habits as well as uptake of prebiotics, probiotics, synbiotics, microbial transplantation, and others.

Open Access March 3, 2022

Seaweeds’ pigments and phenolic compounds with antimicrobial potential

Louisa Gomes, Pedro Monteiro, João Cotas, Ana M. M. Gonçalves, Chantal Fernandes, Teresa Gonçalves, Leonel Pereira

Page range: 89-102

More Download PDF

Abstract

Recently, there has been increased interest in the development of novel antimicrobial compounds for utilization in a variety of sectors, including pharmaceutical, biomedical, textile, and food. The use, overuse, and misuse of synthetic compounds or derivatives have led to an increase of pathogenic microorganisms gaining resistance to the traditional antimicrobial therapies, which has led to an increased need for alternative therapeutic strategies. Seaweed are marine organisms that can be cultivated sustainably, and they are a source of polar molecules, such as pigments and phenolic compounds, which demonstrated antimicrobial potential. This review focuses on current knowledge about pigments and phenolic compounds isolated from seaweeds, their chemical characteristics, antimicrobial bioactivity, and corresponding mechanism of action.

Open Access April 19, 2022

The capture of host cell’s resources: The role of heat shock proteins and polyamines in SARS-COV-2 (COVID-19) pathway to viral infection

Xolani Henry Makhoba, Stanley Makumire

Page range: 220-229

More Download PDF

Abstract

The exposure of organisms and cells to unfavorable conditions such as increased temperature, antibiotics, reactive oxygen species, and viruses could lead to protein misfolding and cell death. The increased production of proteins such as heat shock proteins (HSPs) and polyamines has been linked to protein misfolding sequestration, thus maintaining, enhancing, and regulating the cellular system. For example, heat shock protein 40 (Hsp40) works hand in hand with Hsp70 and Hsp90 to successfully assist the newly synthesized proteins in folding properly. On the other hand, polyamines such as putrescine, spermidine, and spermine have been widely studied and reported to keep cells viable under harsh conditions, which are also involved in cell proliferation, differentiation, and growth. Polyamines are found in all living organisms, including humans and viruses. Some organisms have developed a mechanism to hijack mammalian host cell machinery for their benefit like viruses need polyamines for infection. Therefore, the role of HSPs and polyamines in SARS-CoV-2 (COVID-19) viral infection, how these molecules could delay the effectiveness of the current treatment in the market, and how COVID-19 relies on the host molecules for its successful infection are reviewed.

Erratum

Open Access February 21, 2022

Erratum to “Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents”

Mahmoud Soliman Abdel-Hamid, Amr Fouda, Hesham Kamal Abo El-Ela, Abbas A. El-Ghamry, Saad El-Din Hassan

Page range: 10-10

Download PDF

Special Issue on XXV Congress of the Italian Society for Pure and Applied Biophysics

Open Access March 3, 2022

Low-temperature librations and dynamical transition in proteins at differing hydration levels

Erika Aloi, Rosa Bartucci, Rita Guzzi

Page range: 81-88

More Download PDF

Abstract

Hydration of water affects the dynamics and in turn the activity of biomacromolecules. We investigated the dependence of the librational oscillations and the dynamical transition on the hydrating conditions of two globular proteins with different structure and size, namely β-lactoglobulin (βLG) and human serum albumin (HSA), by spin-label electron paramagnetic resonance (EPR) in the temperature range of 120–270 K. The proteins were spin-labeled with 5-maleimide spin-label on free cysteins and prepared in the lyophilized state, at low ( h = 0.12) and full ( h = 2) hydration levels in buffer. The angular amplitudes of librations are small and almost temperature independent for both lyophilized proteins. Therefore, in these samples, the librational dynamics is restricted and the dynamical transition is absent. In the small and compact beta-structured βLG, the angular librational amplitudes increase with temperature and hydrating conditions, whereas hydration-independent librational oscillations whose amplitudes rise with temperature are recorded in the large and flexible alpha-structured HSA. Both βLG and HSA at low and fully hydration levels undergo the dynamical transition at about 230 K. The overall results indicate that protein librational dynamics is activated at the low hydration level h = 0.12 and highlight biophysical properties that are common to other biosamples at cryogenic temperatures.

Open Access March 14, 2022

The phosphoinositide PI(3,5)P₂ inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Antonella Gradogna, José M. Pardo, Armando Carpaneto

Page range: 119-125

More Download PDF

Abstract

In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional characterization of nonelectrogenic potassium/proton vacuolar antiporters of the NHX (Na + /H + exchanger) family. Moreover, we review the functional role of the tonoplast-specific phosphoinositide PI(3,5)P 2 , able to simultaneously inhibit the activity of NHXs and CLC-a transporters, whose coordinated action can play an important role in the water balance of plant cells.

Open Access March 19, 2022

Targeted photoimmunotherapy for cancer

Andrea Mussini, Eleonora Uriati, Paolo Bianchini, Alberto Diaspro, Luigi Cavanna, Stefania Abbruzzetti, Cristiano Viappiani

Page range: 126-147

More Download PDF

Abstract

Photodynamic therapy (PDT) is a clinically approved procedure that can exert a curative action against malignant cells. The treatment implies the administration of a photoactive molecular species that, upon absorption of visible or near infrared light, sensitizes the formation of reactive oxygen species. These species are cytotoxic and lead to tumor cell death, damage vasculature, and induce inflammation. Clinical investigations demonstrated that PDT is curative and does not compromise other treatment options. One of the major limitations of the original method was the low selectivity of the photoactive compounds for malignant over healthy tissues. The development of conjugates with antibodies has endowed photosensitizing molecules with targeting capability, so that the compounds are delivered with unprecedented precision to the site of action. Given their fluorescence emission capability, these supramolecular species are intrinsically theranostic agents.

Open Access March 21, 2022

Calorimetry of extracellular vesicles fusion to single phospholipid membrane

Miriam Grava, Sally Helmy, Mario Gimona, Pietro Parisse, Loredana Casalis, Paola Brocca, Valeria Rondelli

Page range: 148-155

More Download PDF

Abstract

Extracellular vesicles (EVs)-mediated communication relies not only on the delivery of complex molecular cargoes as lipids, proteins, genetic material, and metabolites to their target cells but also on the modification of the cell surface local properties induced by the eventual fusion of EVs’ membranes with the cells’ plasma membrane. Here we applied scanning calorimetry to study the phase transition of single phospholipid (DMPC) monolamellar vesicles, investigating the thermodynamical effects caused by the fusion of doping amounts of mesenchymal stem cells-derived EVs. Specifically, we studied EVs-induced consequences on the lipids distributed in the differently curved membrane leaflets, having different density and order. The effect of EV components was found to be not homogeneous in the two leaflets, the inner (more disordered one) being mainly affected. Fusion resulted in phospholipid membrane flattening associated with lipid ordering, while the transition cooperativity, linked to membrane domains’ coexistence during the transition process, was decreased. Our results open new horizons for the investigation of the peculiar effects of EVs of different origins on target cell membrane properties and functionality.

Open Access March 24, 2022

Calcium signaling in prostate cancer cells of increasing malignancy

Carla Marchetti

Page range: 156-163

More Download PDF

Abstract

Calcium signaling controls a large variety of cell functions, including proliferation and apoptosis, and plays a major role in neoplastic transformation. Prostate cancer (PCa) is one of the most common malignancies in men. The transition to castration-resistant prostate cancer (CRPC), a lethal form that is still lacking an effective cure, could be influenced by fine tuning intracellular calcium ([Ca 2+ ] i ) homeostasis. This study investigates [Ca 2+ ] i dynamics in metastatic PCa cell lines that mimic the progression of PCa to CRPC: (i) well differentiated LNCaP cells that require androgen for survival, and (ii) poorly differentiated, highly aggressive androgen-insensitive prostate cancer (AIPC) PC3 and DU145 cells. In AIPC cells, ATP induces a fast rise in [Ca 2+ ] i , due to release from intracellular stores and sensitive to phospholipase C inhibitors, while LNCaP cells do not respond to ATP challenge. Moreover, AIPC cells showed a reduced capacity to store Ca 2+ in thapsigargin-sensitive stores and limited store-operated calcium entry, with respect to androgen-dependent LNCaP cells. Finally, green tea extract causes [Ca 2+ ] i elevation and inhibits proliferation in PC3 and DU145 cells, but is ineffective in LNCaP cells. The consequences of these differences are discussed and interpreted in this study with reference to previously proposed models for Ca 2+ dependence of prostate carcinogenesis.

Open Access March 25, 2022

Oxygen diffusion pathways in mutated forms of a LOV photoreceptor from Methylobacterium radiotolerans: A molecular dynamics study

Rocco Zerlotti, Aba Losi, Eugenia Polverini

Page range: 164-174

More Download PDF

Abstract

Mr 4511 from Methylobacterium radiotolerans is a photoreceptor of the light, oxygen voltage (LOV) family, binding flavin mononucleotide (FMN) as a chromophore. It exhibits the prototypical LOV photocycle, with the reversible formation of an FMN-Cys71 adduct via fast decay of the FMN triplet state. Mr 4511 has high potential as a photosensitiser for singlet oxygen (SO) upon mutation of C71. Mr 4511-C71S shows a triplet lifetime ( τ T ) of several hundreds of microseconds, ensuring efficient energy transfer to dioxygen to form SO. In this work, we have explored the potential diffusion pathways for dioxygen within Mr 4511 using molecular dynamics (MD) simulations. The structural model of wild-type (wt) Mr 4511 showed a dimeric structure stabilised by a strong leucine zipper at the two C-terminal helical ends. We then introduced in silico the C71S mutation and analysed transient and persistent oxygen channels. MD simulations indicate that the chromophore binding site is highly accessible to dioxygen. Mutations that might favour SO generation were designed based on their position with respect to FMN and the oxygen channels. In particular, the C71S-Y61T and C71S-Y61S variants showed an increased diffusion and persistence of oxygen molecules inside the binding cavity.

Open Access March 29, 2022

A photosensitizing fusion protein with targeting capabilities

Stefano Bruno, Marilena Margiotta, Marco Cozzolino, Paolo Bianchini, Alberto Diaspro, Luigi Cavanna, Massimiliano Tognolini, Stefania Abbruzzetti, Cristiano Viappiani

Page range: 175-182

More Download PDF

Abstract

The photodynamic treatment for antimicrobial applications or anticancer therapy relies on reactive oxygen species generated by photosensitizing molecules after absorption of visible or near-infrared light. If the photosensitizing molecule is in close vicinity of the microorganism or the malignant cell, a photocytotoxic action is exerted. Therefore, the effectiveness of photosensitizing compounds strongly depends on their capability to target microbial or cancer-specific proteins. In this study, we report on the preparation and preliminary characterization of human recombinant myoglobin fused to the vasoactive intestinal peptide to target vasoactive intestinal peptide receptor (VPAC) receptors. Fe-protoporphyrin IX was replaced by the photosensitizing compound Zn-protoporphyrin IX. Taking advantage of the fluorescence emission by Zn-protoporphyrin IX, we show that the construct can bind prostate cancer cells where the VPAC receptors are expressed.

Open Access March 31, 2022

Ion channels and neuronal excitability in polyglutamine neurodegenerative diseases

Vladimir A. Martinez-Rojas, Leon J. Juarez-Hernandez, Carlo Musio

Page range: 183-199

More Download PDF

Abstract

Polyglutamine (polyQ) diseases are a family composed of nine neurodegenerative inherited disorders (NDDs) caused by pathological expansions of cytosine-adenine-guanine (CAG) trinucleotide repeats which encode a polyQ tract in the corresponding proteins. CAG polyQ repeat expansions produce neurodegeneration via multiple downstream mechanisms; among those the neuronal activity underlying the ion channels is affected directly by specific channelopathies or indirectly by secondary dysregulation. In both cases, the altered excitability underlies to gain- or loss-of-function pathological effects. Here we summarize the repertoire of ion channels in polyQ NDDs emphasizing the biophysical features of neuronal excitability and their pathogenic role. The aim of this review is to point out the value of a deeper understanding of those functional mechanisms and processes as crucial elements for the designing and targeting of novel therapeutic avenues.

Open Access April 8, 2022

Is styrene competitive for dopamine receptor binding?

Emiliano De Santis, Velia Minicozzi, Giancarlo Rossi, Francesco Stellato, Silvia Morante

Page range: 200-206

More Download PDF

Abstract

The potential role of styrene oxide in altering the dopaminergic pathway in the ear is investigated by means of molecular docking and molecular dynamics simulations. We estimate the binding affinity of both styrene oxide and dopamine to the dopaminergic receptor DrD2 by computing the free-energy difference, ∆ G , between the configuration where the ligand is bound to the receptor and the situation in which it is “infinitely” far away from it. The results show that the styrene oxide has a somewhat lower affinity for binding with respect to dopamine, which, however, may not be enough to prevent exogenous high concentration styrene oxide to compete with endogenous dopamine for DrD2 binding.

Open Access April 13, 2022

Diffusion of molecules through nanopores under confinement: Time-scale bridging and crowding effects via Markov state model

Igor V. Bodrenko, Stefan Milenkovic, Matteo Ceccarelli

Page range: 207-219

More Download PDF

Abstract

Passive transport of molecules through nanopores is characterized by the interaction of molecules with pore internal walls and by a general crowding effect due to the constricted size of the nanopore itself, which limits the presence of molecules in its interior. The molecule–pore interaction is treated within the diffusion approximation by introducing the potential of mean force and the local diffusion coefficient for a correct statistical description. The crowding effect can be handled within the Markov state model approximation. By combining the two methods, one can deal with complex free energy surfaces taking into account crowding effects. We recapitulate the equations bridging the two models to calculate passive currents assuming a limited occupancy of the nanopore in a wide range of molecular concentrations. Several simple models are analyzed to clarify the consequences of the model. Eventually, a biologically relevant case of transport of an antibiotic molecule through a bacterial porin is used to draw conclusions (i) on the effects of crowding on transport of small molecules through biological channels, and (ii) to demonstrate its importance for modelling of cellular transport.

Open Access April 21, 2022

Quantitative active super-resolution thermal imaging: The melanoma case study

Mario Marini, Margaux Bouzin, Riccardo Scodellaro, Laura D’Alfonso, Laura Sironi, Francesca Granucci, Francesca Mingozzi, Giuseppe Chirico, Maddalena Collini

Page range: 242-255

More Download PDF

Abstract

Super-resolution image acquisition has turned photo-activated far-infrared thermal imaging into a promising tool for the characterization of biological tissues. By the sub-diffraction localization of sparse temperature increments primed by the sample absorption of modulated focused laser light, the distribution of (endogenous or exogenous) photo-thermal biomarkers can be reconstructed at tunable ∼10−50 μm resolution. We focus here on the theoretical modeling of laser-primed temperature variations and provide the guidelines to convert super-resolved temperature-based images into quantitative maps of the absolute molar concentration of photo-thermal probes. We start from camera-based temperature detection via Stefan–Boltzmann’s law, and elucidate the interplay of the camera point-spread-function and pixelated sensor size with the excitation beam waist in defining the amplitude of the measured temperature variations. This can be accomplished by the numerical solution of the three-dimensional heat equation in the presence of modulated laser illumination on the sample, which is characterized in terms of thermal diffusivity, conductivity, thickness, and concentration of photo-thermal species. We apply our data-analysis protocol to murine B16 melanoma biopsies, where melanin is mapped and quantified in label-free configuration at sub-diffraction 40 µm resolution. Our results, validated by an unsupervised machine-learning analysis of hematoxylin-and-eosin images of the same sections, suggest potential impact of super-resolved thermography in complementing standard histopathological analyses of melanocytic lesions.

Open Access May 23, 2022

Innovative light sources for phototherapy

Giovanni Romano, Giacomo Insero, Santi Nonell Marrugat, Franco Fusi

Page range: 256-271

More Download PDF

Abstract

The use of light for therapeutic purposes dates back to ancient Egypt, where the sun itself was an innovative source, probably used for the first time to heal skin diseases. Since then, technical innovation and advancement in medical sciences have produced newer and more sophisticated solutions for light-emitting sources and their applications in medicine. Starting from a brief historical introduction, the concept of innovation in light sources is discussed and analysed, first from a technical point of view and then in the light of their fitness to improve existing therapeutic protocols or propose new ones. If it is true that a “pure” technical advancement is a good reason for innovation, only a sub-system of those advancements is innovative for phototherapy. To illustrate this concept, the most representative examples of innovative light sources are presented and discussed, both from a technical point of view and from the perspective of their diffusion and applications in the clinical field.

Open Access June 8, 2022

Electrophysiological study of the effects of side products of RuBi-GABA uncaging on GABA_A receptors in cerebellar granule cells

Elena Gatta, Virginia Bazzurro, Elena Angeli, Annalisa Salis, Gianluca Damonte, Aroldo Cupello, Mauro Robello, Alberto Diaspro

Page range: 289-297

More Download PDF

Abstract

The study of the GABA A receptor itself and its pharmacology is of paramount importance for shedding light on the role of this receptor in the central nervous system. Caged compounds have emerged as powerful tools to support research in this field, as they allow to control, in space and time, the release of neurotransmitters enabling, for example, to map receptors’ distribution and dynamics. Here we focus on γ-aminobutyric acid (GABA)-caged compounds, particularly on a commercial complex called RuBi-GABA, which has high efficiency of uncaging upon irradiation at visible wavelengths. We characterized, by electrophysiological measurements, the effects of RuBi-GABA on GABA A receptors of rat cerebellar granule cells in vitro. In particular, we evaluated the effects of side products obtained after RuBi-GABA photolysis. For this purpose, we developed a procedure to separate the “RuBi-cage” from GABA after uncaging RuBi-GABA with a laser source; then, we compared electrophysiological measurements acquired with and without administering the RuBi-cage in the perfusing bath. In conclusion, to investigate the role of the “cage” molecules both near and far from the cell soma, we compared experiments performed changing the distance of the uncaging point from the cell.

Open Access December 9, 2022

Subcellular elements responsive to the biomechanical activity of triple-negative breast cancer-derived small extracellular vesicles

Beatrice Senigagliesi, Diana E. Bedolla, Giovanni Birarda, Michele Zanetti, Marco Lazzarino, Lisa Vaccari, Pietro Parisse, Loredana Casalis

Page range: 322-333

More Download PDF

Abstract

Triple-negative breast cancer (TNBC) stands out for its aggressive, fast spread, and highly metastatic behavior and for being unresponsive to the classical hormonal therapy. It is considered a disease with a poor prognosis and limited treatment options. Among the mechanisms that contribute to TNBC spreading, attention has been recently paid to small extracellular vesicles (sEVs), nano-sized vesicles that by transferring bioactive molecules to recipient cells play a crucial role in the intercellular communication among cancer, healthy cells, and tumor microenvironment. In particular, TNBC-derived sEVs have been shown to alter proliferation, metastasis, drug resistance, and biomechanical properties of target cells. To shed light on the molecular mechanisms involved in sEVs mediation of cell biomechanics, we investigated the effects of sEVs on the main subcellular players, i.e., cell membrane, cytoskeleton, and nuclear chromatin organization. Our results unveiled that TNBC-derived sEVs are able to promote the formation and elongation of cellular protrusions, soften the cell body, and induce chromatin decondensation in recipient cells. In particular, our data suggest that chromatin decondensation is the main cause of the global cell softening. The present study added new details and unveiled a novel mechanism of activity of the TNBC-derived sEVs, providing information for the efficient translation of sEVs to cancer theranostics.

Volume 8 (2017)

Volume 7 (2016)

Volume 6 (2015)

Volume 5 (2014)

Volume 4 (2013)

Volume 3 (2012)

Volume 2 (2011)

Volume 1 (2010)

Cite Score	5.6
Index Copernicus Value	129.82
SCImago Journal Rank	0.730
Source Normalized Impact per Paper	1.387

Submit

MANUSCRIPTS
Biomolecular Concepts encourages the submission of both substantial full-length bodies of work and shorter manuscripts that report novel findings. There are no specific length restrictions for the overall manuscript or individual sections; however, we urge the authors to present and discuss their findings in a concise and accessible manner.

All submissions must be made via online submission system Editorial Manager. In case of problems, please contact the Managing Editor of this journal (jedrzej.daszkiewicz@degruyter.com).

For detailed information, please see Instruction for Authors.

EDITORIAL POLICY
Unpublished material: Submission of a manuscript implies that the work described is not copyrighted, published or submitted elsewhere, except in abstract form. The corresponding author should ensure that all authors approve the manuscript before its submission.
Ethical conduct of research: The authors must describe and confirm safeguards to meet ethical standards when applicable. See Editorial Policy for details.
Conflict of interest: When authors submit a manuscript, they are responsible for recognizing and disclosing financial and/or other conflicts of interest that might bias their work and/or could inappropriately influence his/her judgment. If no specified acknowledgement is given, the Editors assume that no conflict of interest exists. Authors are encouraged to fill in the ICMJE Conflicts of Interest Form (available here) and send it in the electronic format to the Managing Editor.
Copyright: All authors retain copyright, unless – due to their local circumstances – their work is not copyrighted. The copyrights are governed by the Creative-Commons Attribution Only license (CC-BY) which is compliant with Plan-S. We may also publish a paper under CC-BY-NC-ND license on author's request. The corresponding author grants the journal the license to use of the article, by signing the License To Publish. Scanned copy of license should be sent to the journal, as soon as possible.
Authorship: Authorship should be limited to those who have made a significant contribution to the conception, design, execution, or interpretation of the reported study. All those who have made significant contributions should be listed as co-authors. Where there are others who have participated in certain substantive aspects of the research project, they should be named in an Acknowledgement section. The corresponding author should ensure that all appropriate co-authors (according to the above definition) and no inappropriate co-authors are included in the author list of the manuscript, and that all co-authors have seen and approved the final version of the paper and have agreed to its submission for publication.
Peer Review process: Our standard policy requires each paper to be reviewed by at least two Referees and the peer-review process is single-blind. The Editors reserve the right to decline the submitted manuscript without review, if the studies reported are not sufficiently novel or important to merit publication in the journal. Manuscripts deemed unsuitable (insufficient originality or of limited interest to the target audience) are returned to the author(s) without review. The Editor seeks advice from experts in the appropriate field. Research articles and communications are refereed by a minimum of two reviewers, review papers by at least three. Authors are requested to suggest persons competent to review their manuscript. However, please note that this will be treated only as a suggestion, and the final selection of reviewers is exclusively the Editor's decision. The final decision of acceptance in made by Managing Editor or, in case of conflict, by the Editor-in-Chief.
Scientific Misconduct: This journal publishes only original manuscripts that are not also published or going to be published elsewhere. Multiple submissions/publications, or redundant publications (re-packaging in different words of data already published by the same authors) will be rejected. If they are detected only after publication, the journal reserves the right to publish a Retraction Note. In each particular case Editors will follow COPE’s Core Practices and implement the advices.

Editorial

Editor-in-Chief
Daniela GIACOMAZZA, Italian National Research Council, Institute of Biophysics, Italy

Honorary Editor, Founder
Pierre JOLLES, Paris, France

Editorial Advisory Board
Biochemistry, Genetics and Molecular Biology
Wlodzimierz BUJALOWSKI, The University of Texas Medical Branch, USA (biomolecular interactions)
Jannette CAREY, Princeton University, USA (biomolecular interactions)
Ekachai CHUKEATIROTE, Mae Fah Luang University, Thailand (Microbial Development, Biotechnology)
Joel EISSENBERG, Saint Louis University School of Medicine, USA (gene activation, gene silencing)
Vincenzo DE FILIPPIS, University of Padova, Italy (biomolecular interactions, protein engineering)
Carlo MUSIO, Italian National Research Council, Italy (neurobiology, brain physiology, ion channels and disease)
Annalisa PASTORE, King's College London, UK (molecular neuroscience)
Lorenzo PINNA, University of Padua, Italy (protein phosphorylation)
Santiago SCHNELL, University of Notre Dame, USA (biometrology, quantitative and theoretical biology)
Molecular Medicine
Govind BHAGAT, Columbia University, USA (pathology, cell biology)
Raimondo DE CRISTOFARO, Catholic University of the Sacred Heart, Italy (genomics, proteomics, pharmacology and toxicology)
Isabelle MANSUY, University and ETH Zürich, Switzerland (neuroepigenetics)
Kevin MORRIS, City of Hope, USA (RNA, gene therapy, epigenetics)
Ronit SHIRI-SVERDLOV, Maastricht University, the Netherlands (metabolism, Inflammation, translational research)

Editorial Office
Jędrzej Daszkiewicz, Managing Editor
Jedrzej.Daszkiewicz@degruyter.com

DE GRUYTER Poland
Bogumila Zuga 32A Str.
01-811 Warsaw, Poland
T: +48 22 701 50 15

(Deutsch)

Type: Journal
Language: English
Publisher: De Gruyter
First published: August 25, 2010
Publication Frequency: 1 Issue per Year
Audience: researchers in the field of biology, including cellular and molecular biology, biochemistry, genetics, epigenetics, neurosciences, developmental biology, molecular medicine, microbiology, plants biology and biotechnology

Biomolecular Concepts

Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.)

Abstract

NADPH-derived ROS generation drives fibrosis and endothelial-to-mesenchymal transition in systemic sclerosis: Potential cross talk with circulating miRNAs

Abstract

Effect of omega-3 fatty acids on the telomere length: A mini meta-analysis of clinical trials

Abstract

Analysis of differentially expressed genes and signaling pathways involved in atherosclerosis and chronic obstructive pulmonary disease

Abstract

The epigenetic dimension of protein structure

Abstract

Gamma-induced mutants of Bacillus and Streptomyces display enhanced antagonistic activities and suppression of the root rot and wilt diseases in pulses

Abstract

Corticosterone potentiates ochratoxin A-induced microglial activation

Abstract

Supercomplex supercomplexes: Raison d’etre and functional significance of supramolecular organization in oxidative phosphorylation

Abstract

Insights into functional connectivity in mammalian signal transduction pathways by pairwise comparison of protein interaction partners of critical signaling hubs

Abstract

The effects of supplementation of Nannochloropsis oculata microalgae on biochemical, inflammatory and antioxidant responses in diabetic rats

Abstract

Interaction of cervical microbiome with epigenome of epithelial cells: Significance of inflammation to primary healthcare

Abstract

Seaweeds’ pigments and phenolic compounds with antimicrobial potential

Abstract

The capture of host cell’s resources: The role of heat shock proteins and polyamines in SARS-COV-2 (COVID-19) pathway to viral infection

Abstract

Erratum to “Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents”

Low-temperature librations and dynamical transition in proteins at differing hydration levels

Abstract

The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Abstract

Targeted photoimmunotherapy for cancer

Abstract

Calorimetry of extracellular vesicles fusion to single phospholipid membrane

Abstract

Calcium signaling in prostate cancer cells of increasing malignancy

Abstract

Oxygen diffusion pathways in mutated forms of a LOV photoreceptor from Methylobacterium radiotolerans: A molecular dynamics study

Abstract

A photosensitizing fusion protein with targeting capabilities

Abstract

Ion channels and neuronal excitability in polyglutamine neurodegenerative diseases

Abstract

Is styrene competitive for dopamine receptor binding?

Abstract

Diffusion of molecules through nanopores under confinement: Time-scale bridging and crowding effects via Markov state model

Abstract

Quantitative active super-resolution thermal imaging: The melanoma case study

Abstract

Innovative light sources for phototherapy

Abstract

Electrophysiological study of the effects of side products of RuBi-GABA uncaging on GABAA receptors in cerebellar granule cells

Abstract

Subcellular elements responsive to the biomechanical activity of triple-negative breast cancer-derived small extracellular vesicles

Abstract

The phosphoinositide PI(3,5)P₂ inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Electrophysiological study of the effects of side products of RuBi-GABA uncaging on GABA_A receptors in cerebellar granule cells