A multilocus assay was used to genotype up to 27 variable sites in 15 genes in French and Italian, presumed to be healthy populations (n=1480, n=162, respectively). These genes are involved in lipid metabolism ( APOE, APOB, APOC3, CETP, LPL, PON ), homocysteine metabolism ( CBS, MTHFR ), blood viscosity ( Fibrinogen, FV ), platelet aggregation ( GpIIIa ), leukocyte adhesion ( SELE ), and renin-angiotensin system ( AT1R, ACE, AGT ). Allele frequencies for all the markers were compared between the two populations. Five allele frequencies differed between the two European countries: APOB 71Ile (p ≤0.001), SELE 98T (p ≤ 0.001), SELE 128Arg (p ≤0.01), APOE ∈4 (p ≤ 0.01) and MTHFR 677T (p ≤0.01), suggesting the existence of a north-south gradient in European allele frequencies. The other allele frequencies : APOC3 −482T, −455C, 1100T, 3175G, 3206G; LPL −93G, 9Asn, 291Ser; CETP 405Val; PON 192Arg ; ACE Del; AGT 235Thr; AT1R 1166C; CBS 278Thr, GpIIIa P1 A2 ; Fibrinogen −455A, FV 506Gln and SELE 554Phe, were similar between the two populations. They were also similar to those observed in other European countries.