Chenopodium ambrosioides , a member of the Chenopodiaceae family, is renowned for its toxic properties. Despite its toxicity, it has been traditionally utilized in various communities, particularly in pediatric contexts, for its vermifuge, antispasmodic, and antipyretic attributes. This study aims to unravel the phytochemical composition present in organic fractions and aqueous extracts obtained from the aerial components of C. ambrosioides . Furthermore, our objective is to evaluate the antioxidant activity of these extracts and fractions, coupled with a comprehensive examination of their toxicological effects. Polyphenols were quantified using the Folin–Ciocalteu reagent, flavonoids via the aluminum trichloride reagent AlCl 3 , and tannins using the vanillin method. Identification of bioactive compounds within the plant specimen was accomplished through GC-MS spectrophotometric analysis. The assessment of antioxidant activity employed DPPH, ferric (Fe 3+ ) ion antioxidant reducing power (FRAP), ABTS, and TAC methods, with quercetin, catechin, and ascorbic acid serving as standards. Dermoprotective activity was studied using the ultraviolet absorption test. The GC-MS analysis conducted on the aqueous extracts (EAI and EAM) and assorted fractions (FCH, FE, FB, and FA) revealed the presence of diverse chemical families encompassing alcohols, acids, terpenes, steroids, and phenolic compounds. The components identified in the investigated samples, including trans -ascaridol glycol, palmitic acid, phenol, octadecadienoic acid, isoascaridol, eicosanoic acid, 2-methoxy-4-vinyl phenol, mexiletine, and thymol, are postulated as potential contributors to the observed antioxidant activity inherent in the plant extracts and fractions. Our findings highlight the remarkable antioxidant potential of Chenopodium ambrosioides , with the ethyl acetate fraction exhibiting the highest activity (IC 50 = 0.54 mg/ml) in the DPPH test. In the FRAP and ABTS tests, the n -butanolic and ethyl acetate fractions demonstrated superior activity (IC 50 = 4.43 mg/ml, 12.9 mg/ml and IC 50 = 1.6 mg/ml, 4.54 mg/ml, respectively). Conversely, the TAC test revealed that the macerated aqueous extract displayed the highest activity (316.33 mg Eq AG/g), followed closely by the n- butanolic fraction (250.67 mg Eq AG/g). These outcomes can be attributed to the abundant presence of phenolic compounds in the n- butanolic and ethyl acetate fractions, as well as the macerated aqueous extract, playing a pivotal role in the observed antioxidant activity. Additionally, our investigation of the dermoprotective activity demonstrated robust efficacy in the ethyl acetate fraction (FE) and the n- butanolic fraction (FB) compared to the standard agents employed (ZnO and methyl salicylate). Overall, our comprehensive studies affirm that the extracts and fractions derived from C. ambrosioides manifest moderate antioxidant activities alongside significant dermoprotective potential, elucidated by the presence of phenolic compounds in moderate quantities within the plant.