Production and process optimization of a thermostable lipase from Burkholderia sp. ZYB002 and the application potential of this lipase for pitch control of thermomechanical pulp (TMP) has been investigated. The optimized physical and nutritional factors of Burkholderia sp. ZYB002 were found to be (all units in%, w v -1 ): 0.5 peptone, 0.025 urea, 0.5 glycerol, 0.1 NaHCO 3 , 0.2 K 2 HPO 4 , 1.5 Tween 80, 1.1 olive oil, four inoculum volume, and initial pH 8.5. The lipase production process was carried out at 30°C at 250 rpm for 36 h in 250 ml Erlenmeyer flasks containing 35 ml fermentation medium and five glass beads. Under the optimized conditions, lipase activity reached 216.5 U ml -1 . In a TMP of Pinus massoniana , 39% of triglycerides were hydrolyzed by the lipase under optimized conditions [pulp concentration 6% (w v -1 ), lipase dose 20 U g -1 based on dry TMP, pulp initial pH 7.5, and 65°C for 30 min]. The lipase in question displayed a great potential for pitch control of TMP.