Foreign animal diseases (FADs) are recognized as a threat to agriculture, with potential devastating effects on our nation’s livestock and poultry industries. As weapons of bioterrorism, FADs also represent a threat to our nation’s homeland security. Veterinarians play a vital role in protecting our nation’s agricultural resources through their ability to identify and promptly respond to FADs, some of which are of public health concern. Veterinarians working in the Commonwealth of Pennsylvania were surveyed to assess their awareness of FADs and their preparedness to recognize and respond to potential cases. Two hundred thirty-seven veterinary practitioners completed an online questionnaire which included demographic and practice characteristics, federal accreditation status, awareness of FAD history in the United States, awareness of current FAD threats, training, and professional experiences with FADs. In the analysis, we identified topics respondents were most familiar, and those which they feel pose the greatest disease threat. We also assessed respondents’ interest in receiving supplemental training on FADs. Results of this survey indicate that most participating Pennsylvania veterinarians are familiar with FADs and have had educational exposure to FADs either during their formal veterinary education or through the United States Department of Agriculture veterinary accreditation program. However, the majority feels unprepared to recognize FADs. Most survey respondents expressed an interest in receiving supplemental training on FADs, and were willing to travel to receive the training.