Amplification through hybrid optical amplifiers (HOAs) is a propitious and proficient technology for high speed and high capacity dense wavelength-division-multiplexing (DWDM) systems. HOAs are intended to improve system reach and to accomplish wide gain bandwidth with enhanced flatness of gain. In this work, an ultradense 16 channel WDM system is demonstrated and performance of diverse hybrid amplifiers is evaluated in terms of output power, Q-factor, gain flatness and BER. Spacing among the WDM channels is 25 GHz in order to make system bandwidth efficient and scrutinized its effect on four wave mixing (FWM) in case of EDFA-EDFA, Raman-EDFA and SOA-EDFA. It is observed that SOA-EDFA is more and Raman-EDFA is less prone to FWM. Moreover, for distance 20 Km–140 Km, Raman-EDFA is optimal configuration for amplification and from 150 Km–200 Km, SOA-EDFA shows better performance. For prolonged link lengths such as beyond 200 Km, EDFA-EDFA is a right hybrid amplifier. In order to achieve maximum gain flatness in proposed architecture, EDFA-EDFA is recommended to use.