This paper aims to examine how librarians construe organizational culture based on employees’ satisfaction, good leadership, and organizational support with knowledge sharing practices relationships. The quantitative research method, using a structured questionnaire comprised of three constructs of Organizational Culture (OC), including employees’ satisfaction, good leadership, and organizational support, and six constructs of Knowledge Sharing (KS) (innovation, collaboration, communication channel, trust, loyalty, and ethics), was considered as the most suitable for this study. Correlation research design and Pearson correlation test were used for examining the relationships between OC and KS with respect to academic librarians. The findings disclosed significant relationships between OC and KS among the study participants. Organizational support followed by good leadership style and employees’ satisfaction are the strong predictors of knowledge sharing practices in the context of this study. This study has some practical implications for academic library administrators to consider the role of effective organizational culture in promoting knowledge sharing practices among LIS professionals. This paper is the first attempt to explore the relationship between OC and KS in library perspective.