Computer-controlled fluid-flow chemical analysis (CC-FCA) was investigated for the determination of trace amounts of toxic pollutants in the environment. For CC-FCA, automated chemical analysis systems were developed by using computer-controllable pumping and valve modules, and polytetrafluorethylene (PTFE) tubing and connectors. The systems demonstrated in this work were a flow injection-type system, a sequential injection-type system, a mini-column pretreatment system (Auto-Pret system), and an Auto-Pret hyphenated with flow injection analysis (FIA) system. Such systems were fully controlled by a computer program; the lab-made programs were written in Visual Basic. The systems can be hyphenated with some detectors, such as a spectrophotometric detector, an electrochemical detector, electrothermal-atomic absorption spectrometry (ET-AAS), a liquid electrode plasma-atomic emission spectrometry (LEP-AES) and inductively coupled plasma (ICP)-AES. Such systems were successfully applied to the determination of trace amounts of toxic pollutants in environmental water samples: they were heavy metal ions (Pb, Cd, Cr, etc.). In this paper, the author aims mainly at investigating the CC-FCA method for the determination of trace amounts of Cr(VI) in environmental water samples by spectrophotometry. The techniques used in this work were FIA, sequential injection analysis (SIA), and Auto-Pret/FIA, which were all computer-controllable. Limits of detection of Cr(VI) by FIA, SIA, and Auto-Pret/FIA were 8 × 10 –9 mol/L (0.4 μg/L), 1.1 × 10 –8 mol/L (0.6 μg/L), and 1.4 × 10 –9 (0.07 μg/L), respectively. The methods were applied to the determination of Cr(VI) in river and drinking waters.