Microbial flocculants affect the aggregation of suspended solutes in solutions, thus, they are a viable alternative to inorganic and organic synthetic flocculants which are associated with deleterious health problems. Moreover, a potential solution for wastewater treatment. The study aimed to produce and characterize a bioflocculant from Proteus mirabilis AB 932526.1 and apply it in domestic wastewater treatment and dye removal. The bioflocculant was extracted using butanol and chloroform (5:2 v/v). Carbohydrates, proteins, and uronic acid were identified using phenol-sulphuric acid, Bradford, and Carbazole essays. The morphology, crystallinity and elemental composition of the purified bioflocculant were determined using a Scanning electron microscope (SEM), X-ray diffraction analysis and SEM energy dispersive elemental detector (SEM-EDX). The antimicrobial properties and dye removal efficiencies were evaluated. About 3.8 g/L yields of the purified bioflocculant were attained. Chemical composition analysis revealed the presence of 65 % carbohydrates, 10 % proteins, and 24 % uronic acids. The bioflocculant displayed an amorphous and crystalline structure. Bioflocculant further shows some remarkable properties as they can be able to inhibit the growth of both Gram-positive and Gram-negative microorganisms. The removal efficiencies of 85 % (COD), 82 % (BOD), and 81 % (SO 4 2− ) in domestic wastewater were achieved. Moreover, the high removal efficiency of staining dyes such as methylene blue (71 %), carbol fuchsin (81 %), safranin (83 %), methylene orange (90 %), and Congo red (90 %) were found. The produced bioflocculant can imply industrial applicability.