Three reactive amphoteric surfactants (M10, M12 and M14) with different alkyl chain lengths have been synthesized using N , N '-dimethyl-1,3-propyldiamine, maleic anhydride and alkyl bromide as raw materials. The compounds had been characterized by mass spectrometry and 1 H-NMR. The critical micelle concentration (CMC) and the corresponding surface tension (γ CMC ) of M10, M12 and M14 were measured at temperatures from 298 K to 313 K. The results showed that the CMC of M10, M12 and M14 are 5,89 × 10 −4 mol L −1 , 2,63 × 10 −4 mol L −1 and 1,35 × 10 −4 mol L −1 . The γ CMC of M10, M12 and M14 are 31.26 mN m −1 , 28.79 mN m −1 , and 26.87 mN m −1 at 298 K respectively. As the temperature rises, the CMC and the minimum molecule area (A min ) increased gradually, but the γ CMC and the maximum surface adsorption capacity (Γ max ) decreased moderately. The micelle aggregation number (N m ) was determined and changed slightly in the temperature range of 298 K–313 K, which showed that the micelle aggregation number was insensitive to the temperature change.