Two new 24-nor-ursane-type triterpenoids, 2 α ,3 β ,19 α -trihydroxy-24-norurs-4(23),12-dien-28-oic acid ( 1 ) and 3 β -acetoxy-2 α ,19 α -dihydroxy-24-norurs-4(23),12-dien-28-oic acid ( 2 ), along with 15 known compounds were isolated from the methanol extract of the twigs of Mostuea hirsuta . While 2-hydroxymethylbenzamide ( 13 ) was isolated for the first time from the natural source, compounds 3–12 are reported here for the first time from the genus Mostuea . Their structures were elucidated by means of spectroscopic analyses including 1D- and 2D-NMR spectroscopy, high-resolution mass spectrometric data as well as comparison with data from the literature. Compounds 1 , 2 , 4–9 and 13 were tested against bacteria, fungi and plant pathogen oomycetes by the paper disk agar diffusion assay resulting in missing to low activities corresponding with minimum inhibitory concentrations (MICs) > 1 mg mL –1 . However, the respective compounds 1 , 2 , 8 , 9 and 13 exhibited moderate cytotoxic activity against the human Caucasian prostate adenocarcinoma cell line PC-3, with IC 50 10.6–16.5 μ m compared to the standard doxorubicin with IC 50 0.9 μ m .