Eleven compounds were isolated and identified from ethanolic extracts of Solanum virginianum fruits, including two new compounds ( 1 – 2 ) and nine known compounds ( 3 – 11 ). Their structures were determined to be melongenaterpene C15- O - β -D-glucopyranoside ( 1 ), (9 Z )-3,7,11,15-tetramethyl -hexadeca-1,6,10-triene-3,5,14,15-tetraol-5- O - β -D-glucopyranoside ( 2 ), actini-dioionoside A ( 3 ), byzantionoside B ( 4 ), citroside A ( 5 ), 7Z-roseoside ( 6 ), matenoside A ( 7 ), megastigmane ( 8 ), dihydrophaseic acid 3′- O - β -D-glucopyranoside ( 9 ), taraxerol ( 10 ), and huzhangoside C ( 11 ). In this paper, NMR spectroscopy was used to study the structures of the compounds, comparing their data with those in the literature. In addition, the potential anti-inflammatory activity of the compounds was also evaluated using the RAW264.7 cell inflammation model induced by lipopolysaccharide (LPS). The terpenoids showed no significant anti-inflammatory activity.