Complexes of type LnM(η2 - pentamethylenediazirine)MʹLm, LnM/MʹLm = (CO)5Cr/Mo(CO)5, (CO)5Cr/Fe(CO)4, (CO)5W/Fe(CO)4 and (CO)5Cr/Mn(CO)25η(-MeC5H4) were prepared from [MʹLm(THF)] or Fe2(CO)9 and (η1 -pentamethylenediazirine)MLn. The structure of the Cr/Mo complex was determined by X-ray analysis. The IR and UV-VIS data resemble those of the corresponding homodi-metal complexes.
Based on the local road traffic conditions in
Beijing, China, this contribution proposes a rapid modeling
method for road traffic noise sources. Since establishing
the standardized experiment fields are expensive, real
roads are used to determine the road traffic noise emission
model in the method. Due to the similarity in the urban
structures in China and Japan, this paper uses the ASJ-
2013 model as a template and replaces its model parameters
with the ones output by an optimization program
which minimizes the sum of absolute errors between the
predicted and the measured LAeq. Real road experiments
are conducted to verify the effectiveness and feasibility of
the modeling method. The mean error of the model deduced
by the method and the ASJ-2013 model is respectively
0.4 dB and 2.6 dB, and the mean absolute error of the
two models is respectively 1.1 dB and 2.6 dB. The results of
the real road experiments show that the road traffic noise
sources deduced by the method are more accurate to conduct
local noise prediction than those of other models.
Among five ribosome-inactivating proteins tested only saporin-S6 could efficiently release the adenine from adenosine 20 of the synthetic oligoribonucleotide (SRD RNA) mimic of the sarcin/ricin domain of rat 28S rRNA with a Km of 9μM and a kcat of approximately 0.4 min−1 at pH 7.6. The optimal pH for the depurination activity of saporin-S6 is 5.0. However, saporin-S6 lost its site-specificity of depurination on SRD RNA around the optimal pH. The non-specific depurination activity of saporin-S6 was dependent on the enzyme concentration and pH conditions. These results are valuable to understand the diversity and the depurination mechanism of ribosome-inactivating proteins.