The team batting second in a day-night cricket match faces different playing conditions to the team batting first. This paper quantifies the effect on the runscoring ability of the team batting second by using the difference in differences estimator. This approach indicates that batting during the evening reduces the expected number of runs scored per over by 0.2. The effect explains around 12% of the total margin of loss for teams in the treatment group who lose the match. It is also found that batting during the evening increases the expected number of wickets lost at any given point in the innings.