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  • Author: F. X. Niell x
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Abstract

The effects of end-of-day light pulses on thallus expansion, cell size, pigmentation and intracellular accumulation of C and N in the red alga Porphyra umbilicalis were analysed. Red (R) light pulses applied at the end of 12 h white light photoperiod for 14 d stimulated thallus expansion and produced an increase in cell size. Growth rate was higher after red (R) than after blue (B) light pulses. Red or blue light affect thallus expansion, but not the thallus weight, and was partially reversed by far-red (FR) light pulses.

Red light pulses promoted chlorophyll a accumulation whereas B light pulses stimulated the accumulation of phycoerythrin, phycocyanin and soluble protein. The effects of R or B light pulses on pigmentation were partially reversed by FR light, except for phycocyanin. Intracellular C content was similar under both R and B light treatments and higher than after FR light pulses and non-irradiated control. However, intracellular N content was higher after end-of-day R than after end-of-day B light pulses. Total absorptance (400-760 nm) of the thalli was higher after B than R light pulses. However, the ratio of total absorptance and concentration of total pigments (chlorophyll and biliproteins), used as index of light absorp­ tion efficiency, was higher after R than after end-of-day B light pulses. The efficiency of light absorption decreased after the application of FR light pulses. These observations explain the higher growth rate, in terms of thallus expansion, after R than after B end-of-day light pulses and FR treatments. The possible action of phytochrome and a B light photoreceptor in the control of growth and pigmentation in Porphyra umbilicalis is suggested.

Abstract

The photosynthetic performance of an estuarine population of Ulva rotundata Bliding was determined under natural radiation during a daily cycle. The primary production was estimated following two methods, oxygen evolution and dissolved inorganic carbon (DIC) uptake calculated from the pH change and alkalinity in the media after incubation in closed bottles. Maximum values of oxygen evolution (261 μmol O2 m−2 min−1) were reached during midday. The daily course of the DIC uptake rates was different since the maximum rate (248±10 μmol C m−2 min−1) was obtained later in the afternoon. In any case, no photoinhibition was detected by either O2 evolution or DIC uptake. Additionally, the parameters of photosynthesis vs. photon fluence density curves were similar in spite of the technique used. During the daily cycle, significant changes in CA activity and percentage of photosynthetic inhibition following the addition of an external CA inhibitor (acetazolamide) were produced. However, these changes appeared to have a limited effect on photosynthesis. On the other hand, chlorophyll a and b contents decreased drastically in the afternoon and there was not a good correlation between pigment content and photosynthesis rates. Taken into account the results obtained, the daily primary production of U. rotundata based on oxygen evolution was 20% higher than that based on DIC uptake (1.8 and 1.5 g C m−2 day−1, respectively).