We have studied the serological relationship among the human immunodeficiency virus type 1 (HIV-1), and three simian immunodeficiency viruses (SIV). SIVagm was isolated from African green monkeys (Cercopithecus aethiops), and compared with the previously described isolates of SIVmac from a rhesus macaque (Macaca mulatta) and SIVsm from a sooty mangabey (Cercocebus atys). With respect to the glycoproteins, the simian viruses represent a subgroup apparently different from HIV . To classify HIV and SIV isolates further, we compared tryptic peptide maps of the core polypeptides p18 and p24 of HIV-2, three HIV-1 and five SIV isolates. Each peptide map was distinguishable, and differences are most prominent between the HIV-1 group and the SIVmac/SIVsm group. HIV-2 is very similar to SIVmac and SIVsm. The three SIVagm isolates form a more heterogeneous group. The p24s of all SIVagms are more similar to the p24s of HIV-1, but with respect to p18, one isolate is similar to HIV-1, while the two others are more related to SIVmac, SIVsm, and HIV-2.
The structural proteins of simian sarcoma virus type 1 and its associated virus (SSV) were analysed; in general they were similar to the corresponding components of murine leukemia viruses (MuLV), i. e. glycoproteins of approximately 69.000 and 71.000 Daltons (gp69/71), proteins corresponding to p15(E) and p12(E) which were identified as envelope, and p31, p15, p12 and p10 proteins identified as internal components. As in MuLV, p12 stained reddish with Coomassie blue but a pl5(C) distinct from p15(E) was not clearly identified. Using antisera specific for individual components of MuLV cross-reactions were observed between the proteins pl5(E), pi2(E) and p31 of SSV and MuLV, and to a minor degree also between their respective major glycoproteins. An antiserum reacting strongly, specifically and almost exclusively with the envelope components of SSV gp69/71, p15 (E) and p12 (E) was prepared in a goat by inoculation of the animal’s own cells, previously transformed in vitro with SSV and grown in goat serum. Comparative studies with this antiserum in complement fixation and Ouchterlony tests confirmed the strong antigenic similarities between SSV and gibbon ape lymphoma virus but did not identify any interspecies reactivity.