The co-assoziation of four nucleoside-derivatives was investigated using NMR spectroscopy. It turned out that the constant of co-association is at least ten times as great as the value for selfassoziation it a Watson-Crick pair was used. Thermodynamic data were calculated from the temperature dependence of the assoziation constant.
In this paper we described a method how to calculate from NMR the association - caused by Η-bonds - of two components. These components may be present in equal concentration and may be partially self-associated.
The may act as Η-donors and acceptors. The method is demonstrated using some nucleoside derivatives as examples.