In the peritoneal fluid of rats hyaluronate is produced after intraperitoneal injection of phytohemagglutinin.
The hyaluronate concentration was quantitated spectrophotometrically after enzymatic degradation by hyaluronatlyase and a maximum amount of 90 mg% hyaluronate was found after intraperitoneal injection. The production of hyaluronate after phytohemagglutinin injection is dependent on the time and concentration of the phytohemagglutinin. Maximum hyaluronate synthesis is about 48 h after phytohemagglutinin, after this time the hyaluronate concentration is decreased.
After phytohemagglutinin stimulation we isolated a hyaluronate protein complex from the peritoneal fluid by cetylpyridinium chloride precipitation followed by chromatography on Biogel P-150. The dominating amino acids were aspartic acid and glutamic acid.