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  • Author: Martin Weinigel x
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Abstract

Nonlinear contrast methods such as two-photon excited autofluorescence (in combination with fluorescence lifetime imaging) and second-harmonic generation have been combined with a further multiphoton contrast mechanism called coherent anti-Stokes Raman scattering. We describe the principle and the instrumentation for its implementation in tomographs for dermatological applications and present multimodal optical skin biopsies.

Applications in Biology and Medicine