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  • Author: Rajesh K. Jain x
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The plant Tephrosia purpurea (Linn) Pers. (Fabaceae) has been used for a long time in folk medicine for the management of several diseases, including ulcers and other internal wounds. The objective of the present study was to screen the flavonoid-rich fraction of Tephrosia purpurea L. aerial parts for its burn wound repairing efficacy based on traditional uses. Flavonoid fraction ointment (5% w/w) was prepared with simple ointment base B.P. for topical application. The silver sulphadiazine ointment (standard) and simple ointment base B.P. (control) was used in partial thickness and full thickness burn wound models. Wound contraction and tensile strength were measured in partial thickness burn, where as full thickness model was used for the determination of protein, hydroxyproline, anti-oxidant enzymes (SOD, CAT and GSH) level in granuloma mass as well as for histopathological study. It was observed that, wound contraction and tensile strength of skin tissues were observed significantly greater (P<0.001) in the case of flavonoid rich fraction when compared to the control group. In the full thickness burn model (on day 9), protein and hydroxyproline contents were found to be significantly higher (P<0.001). Presence of matured collagen fibers and fibroblast cells with better angiogenesis was observed as a result of histopathological studies, which was visibly improved in case of treated groups compared to control group. In conclusion, our findings suggest that flavonoid-rich fraction may have a potential benefit in enhancing the burn wound healing process, possibly due to the free radical scavenging property of the plant.