The maximum assembling for some period on nested routing platforms of urban rail transit line were calculated by trend analysis method. Based on influencing factors of maximum assembling on platform, relative hypotheses were given. Platforms were divided to eight types by relations between platform and routing train. Interaction of passenger, train and platform, assembling change process, and calculation frames of maximum assembling on different type platforms carried out to be maximum assembling were concluded by trend analysis method. The numerical example application revealed that trend analysis method to calculate maximum assembling on nested routing platforms is feasible, and the final results by trend analysis method are more accurate than by one of codes for design of metro.
We examined the composition and antimicrobial activity of two essential oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei. GC-FID and GC-MS analyses identifi ed 48 and 39 compounds, which represented 95.56% and 94.58%, respectively, of all components in these oils. Of these, 28 compounds were common to both, with a relatively high amount of oxygenated monoterpenes (50.95% and 39.97%). Antimicrobial properties were evaluated in vitro via disc diffusion and microbroth dilution assays. Activities were strong against most tested microorganisms, with inhibition zones ranging from 8.1 to 22.2 mm. For both species, minimum values for inhibitory and bactericidal concentrations were 0.39 to 12.50 mg/mL and 0.78 to 50.00 mg/mL, respectively. These results suggest that these essential oils are potent natural sources of antimicrobial agents for the medicinal and pharmaceutical industries
Haemophilus parasuis is an important bacterium affecting pigs, causing Glässer’s disease. To further characterize this species, we determined the complete genomic sequence of H. parasuis CL120103, which was isolated from diseased pigs. The strain H. parasuis CL120103 was identified as serovar 2. The size of the largest scaffold is 2,326,318 bp and contains 145 large contigs, with the N50 contig being 20,573 bp in length. The complete genome of H. parasuis CL120103 is 2,305,354 bp in length with 39.97% GC content and contains 2227 protein-coding genes, 19 ribosomal rRNA operons and 60 tRNA genes. Sequence similarity of the genome of H. parasuis CL120103 to the previously sequenced genome of H. parasuis was up to 96% and query cover to 86%. Annotation of the genome of H. parasuis CL120103 identified a number of genes encoding potential virulence factors. These virulence factors are involved in metabolism, adhesion, secretion and LPS biosynthesis. These related genes pave the way to better understand mechanisms underlying metabolic capabilities. The comprehensive genetic and phylogenetic analysis shows that H. parasuis is closely related to Actinobacillus pleuropneumoniae and provides a foundation for future experimental confirmation of the virulence and pathogen-host interactions in H. parasuis.
The severity of β-thalassemia syndrome is associated with precipitation of the cytotoxic excessive free α-hemoglobin. We have established a therapy strategy aiming at stabilizing excessive free α-hemoglobin by decreasing its precipitation using erythroid differentiation-related factor (EDRF). To identify the functional EDRF promoter, different length of human EDRF promoter sequence was cloned upstream of green fluorescence protein (GFP) to drive GFP expression. After transfection, the intensity of GFP expression was monitored by microscopy and fluorescence activated cell sorter analysis. The 372 base pair sequence (−116∼+256 bp) was found to be most effective to induce the GFP expression and thereby cloned into pcDNA-EDRF vector to drive EDRF expression. After introducing pcDNA-EDRF vector into MEL cells and healthy mice, we confirmed high EDRF expression at both mRNA and protein levels using reverse transcription polymerase chain reacion and enzyme-linked immunosorbent assay. We further tested the function of EDRF overexpression in β-thalassemia mice by pcDNA-EDRF injection. One week later after treatment, the hemoglobin value, poikilocytosis and target cells of β-thalassemia mice were found to be ameliorated obviously. These results indicated that EDRF is a potential therapeutic target by improving hematological parameters of β-thalassemia.
In this paper, the bottom fire behaviour of 25 cm × 25 cm × 5 cm natural rubber (NR) latex foam with uniformly distributed 6 mm diameter holes was investigated experimentally in a small-scale experimental platform under bottom ventilation. The bottom fire behaviour was analysed. The results show that the burning process of the thermally thick NR latex foam under bottom ventilation conditions can be divided into three stages: initial growing, full development, and decay. A deflagration covered the entire rear surface was observed at 308 s. The burning balls moving at a speed of 0.15 m/s were observed after the bottom ignition and they moved from the center to the sides along with the expansion. The mass loss rate of the sample was accelerated dramatically from 0.2 g/s to 0.5 g/s when the bottom surface was ignited at 308 s.
CYP2E1 mRNA Expression, Genetic Polymorphisms in Peripheral Blood Lymphocytes and Liver Abnormalities in Chinese VCM-Exposed Workers
Objective: To study the relationship between expression of cytochrome P4502E1 (CYP2E1) in human lymphocytes, variant CYP2E1 genotype, exposure to vinyl chloride monomer (VCM), and liver abnormalities in VCM-exposed workers. Methods: A case-control study was performed on 90 male occupationally exposed workers and 42 matched male nonexposed controls. Data were collected based on health surveillance, workplace investigation and questionnaire Survey. Total RNA and DNA were isolated from peripheral blood lymphocytes, and CYP2E1 mRNA expression was determined using RT-PCR, and the presence of CYP2E1 polymorphisms was identified based on PCR-RFLP. Results: The mRNA expression of CYP2E1 in exposed workers (0.89±0.46) was significantly higher than in nonexposed controls (0.61±0.35) (P < 0.01). Logistic regression analysis demonstrated a statistically significant association between CYP2E1 mRNA expression levels and liver abnormalities in the VCM-exposed workers (OR = 3.66, P < 0.05). The genotype frequency for CYP2E1 variants among VCM-exposed workers was not significantly different between workers with liver abnormalities and those without. Conclusions: Liver abnormalities in subjects exposed to VCM are positively associated with expression of peripheral blood lymphocyte mRNA, which is significantly increased in exposed workers compared to nonexposed controls. Therefore, CYP2E1 mRNA levels may be useful for health surveillance and protection of VCM-exposed workers.
The blade deformation caused by aerodynamic and centrifugal loads during operating makes blade configurations different from their stationary shape. Based on the load incremental approach, a novel pre-deformation method for cold blade shape is provided in order to compensate blade deformation under running. Effect of nonlinear blade stiffness is considered by updating stiffness matrix in response to the variation of blade configuration when calculating deformations. The pre-deformation procedure is iterated till a converged cold blade shape is obtained. The proposed pre-deformation method is applied to a transonic compressor rotor. Effect of load conditions on blade pre-deformation is also analyzed. The results show that the pre-deformation method is easy to implement with fast convergence speed. Neither the aerodynamic load nor centrifugal load can be neglected in blade pre-deformation.