Purification of Murine and Feline Type-C Virus
Envelope Polypeptides as Micellar Protein
Josef Schneider*, Heinz Falk **,
and Gerhard Hunsmann*
Z. Naturforsch. 36 c, 353-356 (1981);
received January 13, 1981
Mammalian Type-C Viruses, Envelope Polypeptides, Puri
fication, MicellarComplexes, Peptide Maps
A technique originally described for the isolation of
Friend leukaemia virus envelope polypeptides  yields
equivalent structures from Moloney leukaemia, AKR and
BALB/c xenotropic virus as well as feline leukaemia virus.
This study pertains to theoretical aspect of membrane and surfactant supported ultrafiltration technique followed by experimental evaluation of rejection percentage (R%) and permeate flux (J). The organic dye malachite green (MG) was removed from water samples with help of micellar solution of sodium dodecyl sulfate (SDS) surfactant on account of effective surfactant-dye interaction. The MG removal from water was result of electrostatic force of attraction between Stern layer of SDS micelles and cationic MG in addition to hydrophobic-hydrophobic interaction. The regenerated cellulose membrane was used to retain enhanced MG-SDS micellar complex from polluted water in stirred ultrafiltration cell. R% of MG increases from 79.3%, 77%, 76% to 97.5%, 95%, 90% for 0.01, 0.1 and 0.2 mM concentrations, respectively. “J” decreases throughout the experiment on account of membrane plugging or concentration polarization. Hydrodynamic radius (Rh) of SDS surfactant was also determined at its post micellar concentrations by dynamic laser light scattering (DLLS) that shows high rejection percentage with increased Rh values.
soluble GFP was not changed (Figure 5B).
However, the diffusion time of the lipophilic rhodamine
derivate TMR in 5 mM SDS was about 200 µs (Figure 6),
showing that the fluorescent TMR particles form micellarcomplexes with SDS. When exposed to SDS, a notable
quenching effect was not seen as the number of mole-
cules in triplet state [Ntriplet (%)], number of molecules per
confocal volume (N/0.25fl), and the signal-to-noise ratio
(CPM and CR) remained constant (Table 2).
GFP-Displaying Baculovirus Particles Analyzed by FCS 1943
Fig. 4 Effect of Detergents on the
: cholesterol acyltransferase activation 219
Tab. J . Apparent kinetic constants for the micellarcomplexes
Apolipoprotein A-I (n =
A-I (n = 11)
χ 1/h χ μπιοί
From linear regression analysis of kinetic data as shown in figure 1 app. Vmax is the reciprocal of the y-intercept; app.
Vmax is the slope of the line; and app. Km is calculated from the other two quantities. The ratio app. Vmax/app. Km was significantly
likely small aggregates of chloro
phyll with a changed geometry as compared to the
Micellarcomplexes of chlorophylls and bac-
teriochlorophylls have gained attention due to the
spectroscopic similarities with (bacterio)chloro-
phyll proteins containing the same pigments [1-8].
It has been argued that these similar properties re
sult from the formation of aggregates with similar
geometry, reflecting an inherent property of the
large aromatic systems, e.g. chlorophyll. This view
has been strengthened recently: Very similar types
electrophoresis (SDS-PAGE) in the absence or presence of dithiothreitol (DTT), an unusually strong reducing agent ( Figure 1 A). Reconstituted apoAIwt exists as a monomer, whereas reconstituted apoAI M exists as both a dimer and a monomer, in accordance with a previous report (Weisgraber et al., 1983). HDL is the smallest of the lipoproteins. Based on morphological observation with transmission electron microscope, we confirmed that the micellarcomplexes of reconstituted apoAIwt or apoAI M with soybean lecithin (SPC) were discoidal particles ranging from 20 to 35 nm. The
were all enhanced from 10 to 100 times. The intensity of the emitted radiation was related to the concentrations of micellarcomplexes. The donors (aminophthalate anion) and the acceptors (fluorescein anion) of the energy could be located at distances approximately corresponding to the diameter of the micelle (1–3 nm). This small diameter facilitates the transfer of electron energy, which can occur up to a distance of 10 nm. The dynamic concentration of the donors and acceptors in the Stern region induced several-fold increases in the intensity of the radiation
cetylpyridinium chloride as a cationic
detergent also has no special advantage.
The effect of salts on the Chromatographie behaviour
of ganglioside mixtures might be caused by a decreased
tendency of these glycolipids to form micellarcomplexes
in the presence of these salts.
+ salt addi t ion
Gmixture Gmixture aM1 aD1a
Fig. 1. Thin-layer chromatography of gangliosides on
precoated silicagel plates (Merck, Germany).
Solvent system chloroform/mcthanol/water 120:70:17
(v/v/v). Left, without salt addition. Right, with 0.02%
(w/v) CaCIo x 2H2O. Mixed gangliosides were obtained
Wahl anzusehen. Nach Fällung mit Perchlorsäure
oder mit Ethanol-Diethylether werden Albumin und -Globuline zuverlässig bestimmt, nach Fällung mit
TiwcArya^Reagenz sämtliche Harnproteine. Bei stark gefärbten Urinen kann mit einem Probenleerwert
Determination of protein in urine — A critical review
Summary: Seven methods for protein determination in urine were systematically checked, viz.
— turbidimetric assay with benzethcmium chloride,
— turbidimetry with trichloroacetic acid of various concenträtions,
— micellarcomplex formation with Coomassie