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Notizen 353 Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Complexes Josef Schneider*, Heinz Falk **, and Gerhard Hunsmann* Z. Naturforsch. 36 c, 353-356 (1981); received January 13, 1981 Mammalian Type-C Viruses, Envelope Polypeptides, Puri­ fication, Micellar Complexes, Peptide Maps A technique originally described for the isolation of Friend leukaemia virus envelope polypeptides [1] yields equivalent structures from Moloney leukaemia, AKR and BALB/c xenotropic virus as well as feline leukaemia virus. The


This study pertains to theoretical aspect of membrane and surfactant supported ultrafiltration technique followed by experimental evaluation of rejection percentage (R%) and permeate flux (J). The organic dye malachite green (MG) was removed from water samples with help of micellar solution of sodium dodecyl sulfate (SDS) surfactant on account of effective surfactant-dye interaction. The MG removal from water was result of electrostatic force of attraction between Stern layer of SDS micelles and cationic MG in addition to hydrophobic-hydrophobic interaction. The regenerated cellulose membrane was used to retain enhanced MG-SDS micellar complex from polluted water in stirred ultrafiltration cell. R% of MG increases from 79.3%, 77%, 76% to 97.5%, 95%, 90% for 0.01, 0.1 and 0.2 mM concentrations, respectively. “J” decreases throughout the experiment on account of membrane plugging or concentration polarization. Hydrodynamic radius (Rh) of SDS surfactant was also determined at its post micellar concentrations by dynamic laser light scattering (DLLS) that shows high rejection percentage with increased Rh values.

soluble GFP was not changed (Figure 5B). However, the diffusion time of the lipophilic rhodamine derivate TMR in 5 mM SDS was about 200 µs (Figure 6), showing that the fluorescent TMR particles form micellar complexes with SDS. When exposed to SDS, a notable quenching effect was not seen as the number of mole- cules in triplet state [Ntriplet (%)], number of molecules per confocal volume (N/0.25fl), and the signal-to-noise ratio (CPM and CR) remained constant (Table 2). GFP-Displaying Baculovirus Particles Analyzed by FCS 1943 Fig. 4 Effect of Detergents on the

: cholesterol acyltransferase activation 219 Tab. J . Apparent kinetic constants for the micellar complexes Apolipoprotein A-I (n = Glycated apolipoprotein 12) A-I (n = 11) Correlation coefficient 0.95 0.85 app. Vroax nmol/h 4.14 3.13 app. nmol 0.78 0.29 Vmax/app. Km χ 1/h χ μπιοί app. Km μτηοΐ/ΐ 5.29 10.82 From linear regression analysis of kinetic data as shown in figure 1 app. Vmax is the reciprocal of the y-intercept; app. Vmax is the slope of the line; and app. Km is calculated from the other two quantities. The ratio app. Vmax/app. Km was significantly lower for

likely small aggregates of chloro­ phyll with a changed geometry as compared to the “standard” ones. Introduction Micellar complexes of chlorophylls and bac- teriochlorophylls have gained attention due to the spectroscopic similarities with (bacterio)chloro- phyll proteins containing the same pigments [1-8]. It has been argued that these similar properties re­ sult from the formation of aggregates with similar geometry, reflecting an inherent property of the large aromatic systems, e.g. chlorophyll. This view has been strengthened recently: Very similar types

electrophoresis (SDS-PAGE) in the absence or presence of dithiothreitol (DTT), an unusually strong reducing agent ( Figure 1 A). Reconstituted apoAIwt exists as a monomer, whereas reconstituted apoAI M exists as both a dimer and a monomer, in accordance with a previous report (Weisgraber et al., 1983). HDL is the smallest of the lipoproteins. Based on morphological observation with transmission electron microscope, we confirmed that the micellar complexes of reconstituted apoAIwt or apoAI M with soybean lecithin (SPC) were discoidal particles ranging from 20 to 35 nm. The

were all enhanced from 10 to 100 times. The intensity of the emitted radiation was related to the concentrations of micellar complexes. The donors (aminophthalate anion) and the acceptors (fluorescein anion) of the energy could be located at distances approximately corresponding to the diameter of the micelle (1–3 nm). This small diameter facilitates the transfer of electron energy, which can occur up to a distance of 10 nm. The dynamic concentration of the donors and acceptors in the Stern region induced several-fold increases in the intensity of the radiation

cetylpyridinium chloride as a cationic detergent also has no special advantage. The effect of salts on the Chromatographie behaviour of ganglioside mixtures might be caused by a decreased tendency of these glycolipids to form micellar complexes in the presence of these salts. + salt addi t ion *·*· Gmixture Gmixture aM1 aD1a Fig. 1. Thin-layer chromatography of gangliosides on precoated silicagel plates (Merck, Germany). Solvent system chloroform/mcthanol/water 120:70:17 (v/v/v). Left, without salt addition. Right, with 0.02% (w/v) CaCIo x 2H2O. Mixed gangliosides were obtained

Wahl anzusehen. Nach Fällung mit Perchlorsäure oder mit Ethanol-Diethylether werden Albumin und -Globuline zuverlässig bestimmt, nach Fällung mit TiwcArya^Reagenz sämtliche Harnproteine. Bei stark gefärbten Urinen kann mit einem Probenleerwert gearbeitet werden. Determination of protein in urine — A critical review Summary: Seven methods for protein determination in urine were systematically checked, viz. — turbidimetric assay with benzethcmium chloride, — turbidimetry with trichloroacetic acid of various concenträtions, — micellar complex formation with Coomassie


) ........................................................... 486 O-Methyltransferase Polymethylated Flavonols of Chrysosplenium americanum. I. Identification and Enzymatic Synthesis ............................................................... 730 Micellar Complexes Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Com­ plexes (N) ............................................................... 353 Microbial Acylamino Acid Amido Hydrolase Co+2-Substituted Acylamino Acid Amido Hy­ drolase from Aspergillus oryzae ......................... 751 Mode of Action The Effect of Clotrimazole