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Protoplast Dielectrophoresis in Axisymmetric Fields Natalia Stoicheva, Iana Tsoneva, and Dimiter S. Dimitrov Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. Z. Naturforsch. 40c, 735 — 739 (1985); received February 4, 1985 Protoplasts, Dielectrophoresis, Axisymmetric Fields Pea protoplasts dielectrophoretic coefficients were measured in alternating electric fields of frequency 1 MHz and voltage 5 V applied between two concentric cylindrical metal electrodes of outer and inner radii 0.24 mm and 1 mm, respectively. They do

TMV Inoculation of Tobacco Protoplasts in the Presence of Protoplast Fusion Agents Alan C. Cassells Department of Botany, University College, Cork, Ireland and Frances M. Cocker Department of Horticulture, Wye College, University of London, Ashford, Kent, U.K. Z. Naturforsch. 35 c, 1057-1061 (1980); received June 24/August 22, 1980 Polyethylene Glycol, Protoplasts, Tobacco Mosaic Virus Two methods used to induce protoplast fusion, involving polyethylene glycol (PEG) and high calcium/high pH, respectively, have been investigated in procedures for the

Phytoalexin Production by Isolated Soybean Protoplasts Hannelore Mieth, Volker Speth, and Jürgen Ebel Biologisches Institut II der Universität Freiburg, Schänzlestr. 1, D-7800 Freiburg, Bundesrepu­ blik Deutschland Z. Naturforsch. 41c, 193—201 (1986); received August 1, 1985 Dedicated to Professor Hans Grisebach on the occasion o f his 60th birthday Protoplasts, Soybean Cell Suspension Cultures, Phytoalexin Production, Phytophthora mega- sperm a f. sp. glycinea, Glucan Elicitor Protoplasts isolated enzymatically from suspension-cultured cells of soybean

Flow Cytometric DNA-Analysis of Plant Protoplasts Stained with DAPI Ingunn Ulrich and Wolfgang Ulrich Institut für Genetik. Universität Hohenheim , Garbenstraße 30, D-7000 Stuttgart-Hohenheim , Bundesrepublik Deutschland Z. Naturforsch. 41c, 1052 — 1056 (1986); received July 23, 1986 Flow Cytometry, Plant Protoplasts, Protoplasts Preparation, D N A , D A PI Protoplasts prepared from leaves of various higher plants were stained with the specific DNA fluorochrome DAPI and measured with a pulse cytophotom eter. DNA distribution curves of protoplasts showed DNA

Light-Induced Ca2+ Influx into Spinach Protoplasts Kirsten Heimann, Georg Kreimer, Michael Melkonian, and Erwin Latzko Botanisches Institut der Universität Münster, Schloßgarten 3, D-4400 Münster, Bundesrepublik Deutschland Z. Naturforsch. 42c, 283—287 (1987); received October 24, 1986 Calcium-Influx, Protoplasts, Spinach, Light-Induced Protoplasts from spinach leaves exhibit a light-induced Ca2+ influx. The half maximum rate of Ca2+ influx is achieved at ~ 5 Wm"2. The action spectrum of this influx is similar to that of photosynthesis. Furthermore, light

Characterization of a Light-Induced Oxygen-Uptake in Tobacco Protoplasts Georg H. Schmid and Pierre Thibault Centre d’Etudes Nucleaires de Cadarache, Departement de Biologie, Service de Radioagronomie, B.P. n° 1, 13115 Saint-Paul-lez-Durance, France Z. Naturforsch. 34 c, 570-575 (1979); received April 2, 1979 Protoplasts, Oxygen-Uptake, Photorespiration, Action Spectrum Protoplasts prepared from the wild type tobacco N. tabacum var. John William’s Broadleaf ex­ hibit photosynthetic oxygen-evolution if the suspension medium is supplemented with bicarbonate. In

Characterization of Some Claviceps Strains Derived from Regenerated Protoplasts Brigitte Schumann, Walter Maier, and Detlef Gröger Institut für Biochemie der Pflanzen der Akademie der Wissenschaften der DDR, DDR-4050 Halle (Saale), Weinberg 3 Z. Naturforsch. 42c, 381—386 (1987); received November 6, 1986 Dedicated to Professor Helmut Simon on the occasion of his 60th birthday Claviceps, Protoplasts Regeneration, Strain Development, Ergot Alkaloids, Enzymes Three ergoline alkaloids producing Claviceps strains easily formed protoplasts when treated with snail

E., (Eds.), Advances in Regulation of Plant Growth and Development, Peres Publishers, Olomouc, 1999 [11] Luo J.P., Mu Q., Gu Y.H., Protoplast culture and paclitaxel production by Taxus yunnanensis, Plant Cell Tiss. Organ Cult., 1999, 59, 25–29 [12] Kim J., Bergervoet J., Raemakers C., Jacobsen E., Visser R., Isolation of protoplasts and culture and regeneration into plants in Alstroemeria, In Vitro Cell. Dev. Biol. — Plant, 2005, 41, 505–510 [13] Winkelmann T., Specht J., Serek M

Notizen 337 Valine-Induced Inhibition of Growth of Haploid Tobacco Protoplasts and Its Reversal by Isoleucine Jean-Pierre Bourgin Institut National de la Recherche Agronomique, Laboratoire de Biologie Cellulaire, Versailles (Z. Naturforsch. 31c, 337—338 [1976] ; received February 27, 1976) Valine-Isoleucine Interaction, Tobacco Protoplasts L-valine inhibits the division of haploid tobacco meso- phyll protoplasts. This inhibition is reversed by simultaneous addition of L-isoleucine to the culture medium. The efficiency of a mutagenic treatment of micro

W. A. Keller and G. Melchers • Effect of High pH and Calcium on Protoplast Fusion 737 The Effect of High pH and Calcium on Tobacco Leaf Protoplast Fusion W. A. Keller * and G. Melchers Max-Planck-Institut für Biologie, Tübingen (Z. Naturforsch. 28 c, 737 — 741 [1973] ; received October 18, 1973) Fusion, protoplasts, high pH and Calcium, complementation High frequencies of fusion were induced between isolated tobacco leaf protoplasts when they were incubated at 37 °C in the presence of 0.05 M CaCl2 in 0.4 m mannitol at pH 10.5. Subsequent to the fusion