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Ethane Formation and Chlorophyll Bleaching in DCMU-Treated Euglena gracilis Cells and Isolated Spinach Chloroplast Lamellae E. F. Elstner and Irene Pils Institut für Botanik und Mikrobiologie, Technische Universität München, D-8000 München 2, Arcisstraße 21 Z. Naturforsch. 34 c, 1040 — 1043 (1979) ; received Chlorophyll Bleaching, Ethane Formation, Euglena gracilis, Herbicidal Action, Lipid Peroxidation Light dependent chlorophyll bleaching and ethane formation in Euglena gracilis cells at slightly acidic pH values above 5.2 are enhanced by DCMU and other

Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach Choloroplast Lamellae J. Firl*, D. Frommeyer, and E. F. Elstner** * Institut für Organische Chemie, ** Institut für Botanik und Mikrobiologie, Technische Universität München, D-8000 München 2 Z. Naturforsch. 36 c, 284-294 (1981); received November 28,1980 Oxygen Activation, Spinach Chloroplast Lamellae, Flavins, Photosynthetic Electron Transport From the water extracts of heat-treated spinach chloroplast lamellae, five compounds were isolated by column chromatography and

amounts of ORF (isolated from spinach chloroplast lamellae) instead of MV. A - A> hydroxylamine oxidation; Q —□, decarboxylation of gly­ oxylate. therefor seems to reduce oxygen by a different mechanism compared to Fd or low potential dyes (like MV), both of which reduce oxygen mono- valently (Fig. 7). Identical effects are observed with chloroplasts or ORF from spinach and from sugar beet leaves, although there seems to be more than one com­ pound in sugar beet leaves which exhibit ORF- activity and which are not bound to the chloroplast lamellae (unpublished

. Effect of DBMIB on hydrogen peroxide forma­ tion by isolated spinach chloroplast lamellae. The reaction mixture contained in 2 ml: 100 (irnol phosphate buffer pH 7.8, 2.5 (i-mol NH 4C1, 2.5 [xmol MgCl2 ana chloroplast la­ mellae with 100 ixg Chi. The reaction was conducted for 10 min at 18 °C in white light (30 Klux). H20 2 was deter­ mined with the aid o f NADH-peroxidase (Boehringer). DBMIB added ( m ) none (control) io- io-5 io- H20 2 produced (in % of control) 100 30 90 280 creasing DBMIB concentrations show an increased inhibition of electron transfer between

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.................................................................... 941 Influence o f Bleaching Herbicides on Chloro­ phyll and Carotenoids ......................................... 1047 PYRIDAZINONES, Their Influence on the Biosynthesis o f Carotenoids and the Metabo­ lism o f Lipids in Plants (Survey o f Literature) 1052 Influence o f Aminotriazol on the Biosynthesis o f Chlorophyll and P h y t o l .................................... 1055 Chlorophyll Bleaching Ethane Formation and Chlorophyll Bleaching in DCMU-Treated Euglena gracilis Cells and Isolated Spinach Chloroplast Lamellae . . . 1040 Chlorophyll Fluorescence

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n t e r sc h ic k -N ie b l e r and H. K. L ic h t e n t h a l e r 276 Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach Chloroplast Lamellae J . F ir l , D. F ro m m ey e r , and E. F. E l s t n e r 284 Which Polypeptides Are Characteristic for Photo­ system I I ? Analysis of Active Photosystem I I Particles from the Blue-Green Alga Anacystis nidulans F. K o e n ig and L . P . V e r n o n 295 Protamines. VII. Circular Dichroism Study of Salmine A I G. M. B o no ra , F . B er ta n zo n , V . M oretto , and C. T

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-mas ............................................... Flavanone Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3 '-Hydroxylase Activity in Antirrhinum majus (Snapdragon) ................. Flavanone Oxidase Oxidation o f Flavanones to Flavones with Flow­ er Extracts o f Antirrhinum majus (Snapdragon) . Flavins Isolation and Identification of an Oxygen Re­ ducing Factor (ORF) from Isolated Spinach Chloroplast Lamellae ........................................... Flavodoxin Electron Transport to Assimilatory Nitrate Re­ ductase in Azotobacter v in e la n d ii .......................... Flavonoid Biosynthesis

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, and T. L o w it z 1036 XII Contents E thane Form ation and Chlorophyll Bleaching in DCMU-Treated Euglena gracilis Cells and Iso­ lated Spinach Chloroplast Lamellae E . F. E l s t n e r and I. P il s 1040 Biological Systems to Assay H erbicidal Bleaching G. S a n d m a n n , K .-J . K u n e r t , and P. B ö g e r 1044 Influence of Bleaching Herbicides on Chlorophyll and Carotenoids K .-J . K u n e r t and P. B ö g e r 1047 Pyridazinones, Their Influence on the Biosynthesis of Carotenoids and the Metabolism of Lipids in P lants F. A. E d e r 1052