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Biol. Chem., Vol. 381, pp. 265 – 268, March 2000 · Copyright © by Walter de Gruyter · Berlin · New York Short Communication Binding of Selenoprotein P to Heparin: Characterization with Surface Plasmon Resonance Gavin E. Arteel1, Sebastian Franken2, Joachim Kappler2 and Helmut Sies1,* 1 Institut für Physiologische Chemie I, 2 Labor für Molekulare Neurobiologie, Neurologische Klinik, und Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universität, D-40001Düsseldorf, Germany * Corresponding author The binding of selenoprotein P to glycosaminoglycans using

Biol. Chem., Vol. 379, pp. 683 - 691, June 1998 · Copyright © by Walter de Gruyter & Co · Berlin · New York Analysis of the Mouse Selenoprotein P Gene Peter Steinert*, Dietmar Bächner8 and Leopold Flohe Department of Physiological Chemistry, Technical University of Braunschweig, Mascheroder Weg 1, D-38124 Braunschweig, Germany * Corresponding author In vertebrates several proteins containing a covalently bound selenocysteine residue have been identified. Among these, selenoprotein P is the most unusual one: depending on the species, 8-12 selenocysteine residues

Summary

Background Glaucoma is a highly prevalent eye disease related to optic nerve lesions and visual field defects. Primary Open-Angle Glaucoma (POAG) is a type of glaucoma that occurs frequently with unknown etiology. In this study, we investigated the serum levels of selenium, selenoprotein P1, glutathione, hemolysate glutathione peroxidase1 (GPx1) activity and aqueous humour selenium in POAG patients.

Methods Ninety sex- and age-matched subjects (POAG patients; n=45 and, controls; n=45) with the controlled confounders (smoking, hypertension and alcohol beverages) were recruited on clinical histories and exams. The serum and aqueous humour selenium levels were measured using GFAAS technique. The serum selenoprotein P1 level was assayed with the ELISA method. The hemolysate GPx1 activity and serum reduced glutathione level were also measured using known colorimetric techniques.

Results The serum selenium (P=0.01) and selenoprotein P1 (P<0.001) levels were significantly high in POAG patients. Furthermore, the aqueous humour selenium level was significantly high among patients as compared to controls (64.68±13.07 vs. 58.36±13.76 ng/mL, P=0.02). The results did not show a significant difference (P=0.36) in the hemolysate GPx1 activity between the groups. The cutoff points for intraocular pressure (IOP) and serum selenoprotein P1 parameters were estimated to be 39 mmHg (sensitivity 97.5%; 1-specificity 6.5%) and 188 mg/mL (sensitivity 93.5%; 1-specificity 14%), respectively.

Biol. Chem., Vol. 388, pp. 1043–1051, October 2007 • Copyright by Walter de Gruyter • Berlin • New York. DOI 10.1515/BC.2007.136 2007/183 Article in press - uncorrected proof Post-translational processing of selenoprotein P: implications of glycosylation for its utilisation by target cells Holger Steinbrenner, Lirija Alili, Dominik Stuhlmann, Helmut Sies and Peter Brenneisen* Institute for Biochemistry and Molecular Biology I, Heinrich Heine University Düsseldorf, Universitätstrasse 1, D-40225 Düsseldorf, Germany *Corresponding author e-mail: PeterBrenneisen

determined here by in situ hybridization and Northern blotting experiments, glutathione peroxidases (GPx) 1 and 4 and selenoprotein P were moderately expressed, occurring selectively in the follicular cells and in leuko- cytes of germinal follicles of thyroids affected by Hashi- moto’s thyroiditis. Selenoprotein 15 was only marginally expressed and distributed over all cell types. GPx3 mRNA was exclusively localized to the thyrocytes, showed the highest expression levels and was down- regulated in 5 of 6 thyroid cancer samples as compared to matched normal controls. GPx3

), selenoprotein P (SeP), leukocyte derived chemotaxin 2 (LECT2), and so on. [ 7 , 8 ] Like the other members of organokines such as adipokine, myokine, hepatokine is defined as proteins or protein-like substances secreted mainly or exclusively by liver in an endocrine or paracrine way. In 2006, the Japanese scholar Hotamisligil put forward a hypothesis about metabolism and inflammation. From a genetic and evolutionary perspective, the liver, adipose and hematopoietic tissue maintain their developmental heritage and share evolutionary underpinnings. There are overlapping

Summary

Metabolic changes resulting from obesity, insulin insensitivity, and imbalances in hormones such as adiponectin, leptin, resistin, apelin and visfatin, which are derived from white adipose tissue-derived hormone, are directly linked to both colon cancer (CC) and inflammatory bowel diseases increasing tissue-derived risk. We conducted this study to evaluate the relationship between the circulating concentrations of adiponectin, leptin, resistin, apelin and visfatin and colon adenoma and CC. Our study included 90 participants aged >18 years who were divided into three groups: colon cancer, adenoma and control. The serum concentrations of the investigated adipohormones were measured with ELISA in 30 patients with colon adenoma, 30 with CC and 30 controls with no colon pathology. Demographic, anthropometric, metabolic and hormonal parameters were also recorded. The group means were compared by using one-way analysis of variance (ANOVA). Dual comparisons between groups were analyzed with the Tukey test. Pearson correlation coefficient was used to determine the relation between continuous variables. Adiponectin and leptin levels in patients with adenomas (p<0.000; p<0.000, respectively) and CC (p<0.000; p<0.000, respectively) were lower than in controls. Apelin level in patients with CC (p<0.000; p<0.000, respectively) was lower than in patients with adenomas and in controls. Resistin and visfatin levels in patients with CC (p<0.000; p<0.000, respectively) were higher than in patients with adenomas and in controls.

Biol. Chem., Vol. 388, pp. 987–995, October 2007 • Copyright by Walter de Gruyter • Berlin • New York. DOI 10.1515/BC.2007.112 2007/187 Article in press - uncorrected proof Review Selenium in mammalian spermiogenesis Leopold Flohé Molisa GmbH, Brenneckestraße 20, D-39118 Magdeburg, Germany e-mail: l.flohe@t-online.de Abstract The role of selenium in male fertility is reviewed with special emphasis on selenoprotein P and phospholipid hydroperoxide glutathione peroxidase (GPx4) in spermio- genesis. Inverse genetics reveal that selenoprotein P is required for

Gastroenterology, Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA * Corresponding author e-mail: Joachim.Herz@UTSouthwestern.edu Abstract Apolipoprotein E receptor 2 (Apoer2) is a multifunctional transport and signaling receptor that regulates the uptake of selenium into the mouse brain and testis through endocytosis of selenoprotein P (Sepp1). Mice deficient in Apoer2 or Sepp1 are infertile, with kinked and hypomotile spermatozoa. They also develop severe neurological defects on a low selenium diet, due to a profound impair- ment of selenium uptake

predisposition and severity and accel- erates recovery in a variety of pathologies. Pre-supple- mentation Se levels and sex represent important determinants of these Se-dependent health effects. Accordingly, we previously reported on sexually dimor- phic expression patterns of Se-dependent glutathione peroxidase 1, type I deiodinase, and selenoprotein P in young mice. In the present study we investigated wheth- er these differences vary with age. The strong sexual dimorphic expression of hepatic type I deiodinase that was observed in young mice vanished both at the mRNA and