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WILD SPECIES: THE WIDER GENE POOL Wild relatives of crops have often played important roles in sustaining agricultural productivity. People have been adopting wild species for thousands of years, and after domestication, some of the resulting cul- tivars have continued to hybridize naturally with their wild relatives. Such spontaneous gene flow helps maintain the vigor of crops and can lead to the development of new crops. Plant breeders have turned to wild species mainly for sources of resistance to diseases, pests, and stressful environments, such as

HELIA, 25, Nr. 36, p.p. 65-78, (2002) UDC 633.854.78:631.527 USING THE COLLECTION OF WILD SPECIES IN SUNFLOWER BREEDING N. Tavoljanski1*, A. Yesaev1, V. Yakutkin2, E. Akhtulova1 and V. Tikhomirov1 1“VIS, Ltd”, Centralnaya 43 “a”, Veidelevka, Belgorod region, 309720, Russia 2All-Russian Plant Protection Institute (VIZR), Shosse Podbelskogo 3, St-Petersburg, Pushkin 196620, Russia Received: November 08, 2001 Accepted: June 03, 2002 SUMMARY We started to collect wild sunflower (Helianthus L.) species in 1998. The main task of this collection is to promote the

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HELIA, 29, Nr. 45, p.p. 11-18, (2006) UDC 633.854.78:631.523 IDENTIFICATION OF WILD SPECIES OF SUNFLOWER BY A SPECIFIC PLASTID DNA SEQUENCE Vischi, M.*, Arzenton, F., De Paoli, E., Paselli, S., Tomat, E., Olivieri, A.M. Dip. Scienze Agrarie ed Ambientali, Università di Udine, Via delle Scienze, 208, I 33100 Udine, Italy Received: October 15, 2006 Accepted: December 05, 2006 SUMMARY Four sunflower species, Helianthus annuus, H. argophyllus, H. debilis and H. tuberosus, were characterized at the molecular level using the plastid trnH-psbA intergenic spacer. The

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HELIA, 33, Nr. 53, p.p. 31-36, (2010) UDC 633.854.78:631.527.53 DOI: 10.2298/HEL1053031O SELF-FERTILIZATION AND RESTORATION TO CYTOPLASMIC MALE STERILITY OF SOME WILD SPECIES OF Helianthus Onemli, F.*1, Gucer, T.2 1 University of Namik Kemal, Faculty of Agriculture, Department of Field Crops, 59030 Tekirdag, Turkey 2 Thrace Agricultural Research Institute, Edirne, Turkey Received: April 10, 2010 Accepted: August 10, 2010 SUMMARY The objectives of this study were to determine self-fertility and restoration capability of cytoplasmic male fertility of some

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HELIA, 33, Nr. 53, p.p. 17-24, (2010) UDC 633.854.78:631.547 DOI: 10.2298/HEL1053017O THE CHARACTERIZATION OF SOME WILD SPECIES OF Helianthus FOR SOME MORPHOLOGICAL TRAITS Onemli, F.*1, Gucer, T.2 1 University of Namik Kemal, Faculty of Agriculture, Department of Field Crops, 59030 Tekirdag, Turkey 2 Thrace Agricultural Research Institute, Edirne, Turkey Received: April 10, 2010 Accepted: August 10, 2010 SUMMARY This present investigation was undertaken to determine some plant char- acters in six wild Helianthus genotypes and their test hybrids in 2007 and

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HELIA, 33, Nr. 53, p.p. 45-54, (2010) UDC 633.854.78:632.112(58.032.3) DOI: 10.2298/HEL1053045O RESPONSE TO DROUGHT OF SOME WILD SPECIES OF Helianthus AT SEEDLING GROWTH STAGE Onemli, F. *1, Gucer, T.2 1 University of Namik Kemal, Faculty of Agriculture, Department of Field Crops, 59030 Tekirdag, Turkey 2 Thrace Agricultural Research Institute, Edirne, Turkey Received: April 10, 2010 Accepted: August 10, 2010 SUMMARY Response of six wild sunflower genotypes including Helianthus petiolaris spp. petiolaris (E-142), Helianthus neglectus (E-017) and

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Abstract

Salmonella spp. is the leading cause of zoonotic enteric diseases wich represents a public health concern worldwide. The incidence of zoo-associated salmonellosis is rather high due to the high prevalence and shedding of this bacterium from wild animals specially kept in stressful conditions. To determine the potential public health risk presented by zoo animals in Semnan, we investigated the prevalence of Salmonella serovars Enteritidis and Typhimurium among wild animal species kept in the zoo and pet shops. Totally, 152 fecal samples from species in the zoo and pet shops were collected and Salmonella prevalence and identification was assessed via standard bacteriologic culture methods, serotyping, multiplex-PCR and antimicrobial susceptibility testing. Overall, 21% (32/152) of the samples were confirmed positive for Salmonella and serotyping showed 12.5% (19/32) Salmonella serovar Enteritidis and 8.5% (13/32) serovar Typhimurium, respectively. All the Salmonella isolates were sensitive to Chloramphenicole, Flurefenicole, Meropenem, Ceftizoxime, Imipenem and Ampicillin, while resistance was observed in the case of Nalidixic acid (78%) as the highest resistance, Streptomycin (28%), Oxytetracycline, Neomycin, Furazolidone (each one 15%) and Lincospectin (9.3%). The high occurrence of multidrug resistance Salmonella in zoo and pet animals represents a potential threat to public health and requires strict surveillance and application of hygienic criteria.

induced somatic embryogenesis from corolla tubes, and obtained secondary somatic embryos and regenerated plants. This will increase the explant types and embryoid induction methods in sunflower. Materials and methods Materials Two interspecific amphiploids (2n=4x=68) between cultivated sunflower and its wild species, G08/2280 ( H. pumilus ×P21), G08/2260 (NMSHA89× H. maximiliani ), and one tetraploid BC 1 F 3 of a hexaploid amphiploid backcrossed with cultivated line, G08/2394 (( H. hirsutus ×P21)×HA89) (2n=4x=68), were used as explant donors for in vitro culture

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HELIA, 26, Nr. 39, p.p. 43-50, (2003) UDC 633.854.78:631.527 CHARACTERIZATION OF INTERSPECIFIC HYBRIDS BETWEEN CULTIVATED SUNFLOWER H. annuus L. (cv. ALBENA) AND WILD SPECIES Helianthus tuberosus Encheva, J.*, M. Christov and P. Ivanov Dobroudja Agricultural Institute, General Toshevo 9520, Bulgaria Received: May 30, 2003 Accepted: October 15, 2003 SUMMARY By using the direct organogenesis method, applied for the first time at the Biotechnology Laboratory of DAI, General Toshevo, new sunflower forms were obtained through distant hybridization from the cross

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