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, experimental variables, and references of behavioral assays that can be adapted to look at stress responses in larval zebrafi sh. Activity rate in 4DPF zebrafish larvae in response to salt change Figure 1 Activity rates in zebrafi sh larvae at 4 days post-fertiliza- tion (dpf) with hyperosmotic shock. Larval movements were recorded on 4 dpf zebrafi sh larvae with embryo medium (control) or embryo medium with NaCl that would increase the NaCl concentration by 31.6 m m , 100 m m , or 316 m m . Embryos were transferred to 48-well microtiter plates at 2 dpf and

disease. Regarding the remaining vertebrate models, construction of the mouse model is expensive and time consuming, more so cat and dog models, rendering them unsuitable for a large-scale drug screening. Therefore, to explore the pathogenesis of NPC1 disease, it is necessary to find a vertebrate model suitable for large-scale drug screening tests. Several characteristics make zebrafish a promising fish model for studying human diseases: ease of breeding in large numbers in the laboratory, short generation time, and production of hundreds of transparent eggs. All this

Journal of Integrative Bioinformatics 2007 http://journal.imbio.de Developmental Anatomy Ontology of Zebrafish: an Integrative semantic framework. Mounia Belmamoune and Fons J. Verbeek 1 Leiden Insititute of Advanced Computer Science (LIACS), Section Imaging & Bio- Informatics, Leiden University, Leiden, the Netherlands Summary Integration of information is quintessential to make use of the wealth of bioinformatics resources. One aspect of integration is to make databases interoperable through well

Abstract

The function of sleep remains a central enigma of modern biology, in spite of the obvious importance of sleep for normal physiology and cognition. The zebrafish has emerged as a promising new model for studying sleep, its changes with age, and the impact of sleep alterations on cognitive function. Recent studies of this diurnal vertebrate have provided new insights into the dual role of the pineal hormone melatonin and its receptors, regulating sleep in diurnal vertebrates through both homeostatic and circadian mechanisms. Research in zebrafish has also revealed interactions between melatonin and the hypocretin/orexin system, another important sleep-wake modulator. Future investigations should benefit from the conservation in zebrafish of mechanisms that regulate normal sleep, our extensive knowledge of their molecular biology, the availability of multiple transgenic and mutant phenotypes, and the feasibility of applying sensitive in vivo imaging techniques to record sleep-related neuronal activity in these optically transparent subjects. The established sensitivity of zebrafish to many pharmacological hypnotics should also contribute to the development of new, safe and effective sleep medications.

Abstract

Zebrafish are at the forefront of neurobiological research and have been gaining popularity as a viable and valid behavioral model in a variety of research applications (e.g., assessing drug induced behavioral changes). This model becomes even more attractive when considering the behavioral changes that follow exposure to compounds that are water-soluble. As such, several studies have implicated both acute and chronic ethanol exposure in the modulation of zebrafish behavior. Within this arena there appears to be a common trend across multiple studies. As with many drugs ethanol appears to influence behavior in a dose-dependent manner. In this review, we compare and contrast several studies that measure behavior as a result of alcohol exposure. Appended to this review are pilot data that report zebrafish blood alcohol concentrations as a function of acute exposure.

Current Directions in Biomedical Engineering 2017; 3(2): 199–202 Markus Reischl*, Andreas Bartschat, Urban Liebel, Jochen Gehrig, Ference Müller and Ralf Mikut ZebrafishMiner: an open source software for interactive evaluation of domain-specific fluorescence in zebrafish Abstract: High-throughput microscopy makes it possible to observe the morphology of zebrafish on large scale to quantify genetic, toxic or drug effects. The image acquisition is done by automated microscopy, images are evaluated automatically by image processing pipelines, tailored

1 Introduction Over the past 25 years, the zebrafish has become a model organism to study cardiogenesis and heart diseases, for fundamental research as well as other biomedical applications [ 1 ], [ 2 ], [ 3 ], [ 4 ]. Due to the transparency of zebrafish embryos throughout embryogenesis, a real time in-vivo observation of physiological or pathological events occurring during organ development is possible [ 4 ], [ 5 ], [ 6 ]. Determination of cardiac contractility is useful to elucidate cardiac and circulation defects in zebrafish embryos. Fractional shortening

to the Moon, Mars, and beyond, it is essential to use state-of-the-art scientific models to further investigate the impact of microgravity on the musculoskeletal system as well as to identify preventive measures mitigating the severe consequences of extended exposure to weightlessness. In fact, the Space Biology Plan for 2016–2025 released by NASA encourages studies designed to investigate the biology effects of long duration space environment exposure ( Tomko et al., 2016 ). In this study, we assessed the behavior of zebrafish larvae as a precursor for the future

preliminary analysis of G. duodenalis population biology in marine systems. International Journal for Parasitology, 40, 1063–1074. DOI: 10.1016/j.ijpara. 2010.02.015. http://dx.doi.org/10.1016/j.ijpara.2010.02.015 [6] Meijer A.H., Spaink H.P. 2011. Host-pathogen interactions made transparent with the zebrafish model. Current Drug Targets, 12, 1000–1017. http://dx.doi.org/10.2174/138945011795677809 [7] Monis P.T., Caccio S.M., Thompson R.C. 2009. Variation in Giardia: towards a taxonomic revision of the genus. Trends in Parasitology, 25, 93–100. DOI: 10.1016/j.pt.2008

Data Integration for Spatio-Temporal Patterns of Gene Expression of Zebrafish development: the GEMS database Mounia Belmamoune1,2 and Fons J. Verbeek1 1Section Imaging and Bioinformatics, Leiden Institute of Computer Science (LIACS), Leiden University, The Netherlands Summary The Gene Expression Management System (GEMS) is a database system for patterns of gene expression. These patterns result from systematic whole-mount fluorescent in situ hy- bridization studies on zebrafish embryos. GEMS is an integrative platform that addresses one of the important